EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11171

Klk10 kallikrein related-peptidase 10 ( MGI:1916790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003602. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003602_01	euxassay_003602_02	euxassay_003602_03	euxassay_003602_04

euxassay_003602_05	euxassay_003602_06	euxassay_003602_07	euxassay_003602_08	euxassay_003602_09

euxassay_003602_10	euxassay_003602_11	euxassay_003602_12	euxassay_003602_13	euxassay_003602_14

euxassay_003602_15	euxassay_003602_16	euxassay_003602_17	euxassay_003602_18	euxassay_003602_19

euxassay_003602_20	euxassay_003602_21	euxassay_003602_22	euxassay_003602_23	euxassay_003602_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11171_wholemount_strong.wlz

11171_wholemount_moderate.wlz

11171_wholemount_weak.wlz

11171_wholemount_possible.wlz

11171_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11171_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lumbar intervertebral disc

strong

regional

strong expression: see section 17

thoracic intervertebral disc

strong

regional

strong expression: see section 15 16

hindlimb

weak

regional

weak expression: see section 02 03 04 22 23 24

vibrissa

moderate

regional

moderate expression: see section 05 21 22 23 weak expression: see section 06 07 24

naris

moderate

regional

moderate expression: see section 12 13 16 17 18

nasal septum

moderate

regional

moderate expression: see section 14

viscerocranium

moderate

regional

Expression in the turbinate bone.

rectum

moderate

regional

moderate expression: see section 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 11 14 15 18

oral epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 20 21 24 weak expression: see section 16 18 19 22

sacral vertebral cartilage condensation

strong

regional

strong expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5960
Entity Detected:	Klk10, kallikrein related-peptidase 10 ( MGI:1916790)
Sequence:	sense strand is shown >T5960 CCCACGCGTCCGCCCACGCGTCCGGTTTGCAGCCCACACTGCAGGGCAAGAGCCACTTCTGAGTCTCCTT TCTTCTTACTGGCGACTCCCAGGCCTCCACTCCAGCCTCTGCAAGCCAAGTATCAGCTATGAGGGTCCCG CTTCTTCACCTCTCCACTGCCTCTGGCTCCTGGTCCCTGGTGAAGCTTCTGCTGCCGCTACTGATGGTGC AACTCTGGGCGGCGCAGGCTCTGCTGCTCCCGGGAAACGCCACGCGCGTGGACCTCGAGGCCTCCGGCGC CCAGTGCGAGCGTGACTATCATCCCTGGCAGGTCTCCCTCTTCCATAACCTCCAGTTCCAATGTGCGGGT GTCCTGGTGGACCAGAACTGGGTGCTCACGGCCGCACATTGCTGGAGAAATAAGCCACTGAGAGCTCGAG TTGGCGATGACCACTTGCTGCTTTTCCAGAAGGAGCAACTGCGGTCCACGAGTTCACCTGTTTTCCACCC CAAGTATCAGGCTTGCTCAGGCCCCATCCTGCCACATCGCTCAGATGAACACGACCTCATGATGCTGAAA CTGAGCAGTCCTGTAATGCTGACGTCCAACGTGCATCCCGTGCAACTGCCCTTCCGCTGCTCTCAGCCAG GGCAAGAGTGTCAGGTCTCAGGCTGGGGGACCTCAGCCAGCCGAAGAGTGAAGTACAATAGGAGCCTGAG CTGTTCCAAGGTAACTCTCCTTAGCCAGAAGCAGTGTGAGACCTTCTACCCAGGTGTGATCACCAACAGC ATGATTTGTGCTGAGGCAGACGGAAACCAAGACTCTTGCCAGAGTGACTCCGGTGGCCCCCTGGTGTGTG ATGACACCCTGCATGGCGTCCTCTCGTGGGGCATTTACCCATGTGGTGCTGCCCAGCACCCATCTGTCTA CTCAGAGATCTGCAAATACACCCCCTGGATCCGAAGAGTCATTCGTTTCAAATGACCGTCTGCCCCTCTC CTCCTCTCATGCCCACCCACCCCTCTTCCTTCATCCCTTGTCCACTCCTGTTCTCTGCTCACAAATGCAG AAAATGGTGGCTGAGAAAGTTCTAGTACCCCAAGGAAGCTAGCAGTCCCCAAGTCTCTAAGAAAAGTTAC CTGTCATTCAACCTCTACCTCCCTCCTCTGTGTGACTGGGACAAGCCTGGTACTCTCTCTGAGCCTTGGT TTCCTTGTCTGTAAAATGGGGACAATGAATTGTCTACCTCTTACATATGTTGTATGGAGACCATGATGGA ATATGTGTGTGTGTGTATGTGTGTGTTTATGGTCACTGACACAGGAACATGAACACCATCTGGTTATCAC TGATTAAACACTACCAAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484608 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484608 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11174 same embryo
	EMAGE:11172 same embryo
	EMAGE:11175 same embryo
	EMAGE:11173 same embryo
	EMAGE:11170 same embryo
	EurExpress:euxassay_003602 same experiment
	MGI:4825810 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source