EMAGE mouse gene expression spatial database

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EMAGE:11172

Efna1 ephrin A1 ( MGI:103236)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003508_01	euxassay_003508_02	euxassay_003508_03	euxassay_003508_04

euxassay_003508_05	euxassay_003508_06	euxassay_003508_07	euxassay_003508_08	euxassay_003508_09

euxassay_003508_10	euxassay_003508_11	euxassay_003508_12	euxassay_003508_13	euxassay_003508_14

euxassay_003508_15	euxassay_003508_16	euxassay_003508_17	euxassay_003508_18	euxassay_003508_19

euxassay_003508_20	euxassay_003508_21	euxassay_003508_22	euxassay_003508_23	euxassay_003508_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11172_wholemount_strong.wlz

11172_wholemount_moderate.wlz

11172_wholemount_weak.wlz

11172_wholemount_possible.wlz

11172_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11172_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 09 22 23 24

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 24

left lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

right lung

moderate

regional

moderate expression: see section 17 18 19 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5959
Entity Detected:	Efna1, ephrin A1 ( MGI:103236)
Sequence:	sense strand is shown >T5959 GGAGAAGCCTGTGGGAACCTTACACTGCAGAGCTCTCGTCCTGGCCTGGCTCGGCCCGGCCCCGCGCTAT GGAGTTCCTTTGGGCCCCTCTCTTGGGTCTGTGCTGCAGTCTGGCCGCTGCTGACCGCCACATCGTCTTC TGGAACAGTTCAAATCCCAAGTTCCGTGAGGAGGACTACACGGTGCACGTGCAGCTGAATGACTACCTAG ACATCATCTGCCCACATTACGAGGACGACTCTGTGGCAGATGCAGCCATGGAGCGATACACACTGTACAT GGTGGAACACCAGGAGTATGTGGCATGCCAACCCCAGTCCAAGGACCAGGTCCGTTGGAATTGCAACCGG CCCAGTGCCAAGCATGGCCCGGAGAAGCTGTCTGAGAAATTCCAGCGCTTCACGCCTTTTATCTTGGGCA AGGAGTTCAAGGAAGGACACAGCTACTACTACATCTCCAAACCTATCTACCATCAGGAATCCCAGTGCTT GAAGCTGAAGGTGACTGTCAATGGCAAAATCACTCATAATCCCCAGGCCCATGTCAACCCACAGGAGAAG AGACTCCAAGCAGATGACCCGGAAGTACAGGTTTTGCACAGCATTGGTTACAGTGCCGCCCCCCGCCTCT TCCCACTGGTCTGGGCAGTATTGCTCCTACCACTGCTGCTGCTGCAATCTCAGTGAGGGTGTACGCTTGC CCTGGCTTATGGATTGGAATGGGACTAAGGGGCAGCCCCAGCCCTGGGAACCTCTCCCACTACACCCACA AGACGCCACCATGAAGCCTCAAAAGGTTCAGTATTAAGGGTTTTAACCGAAAAGAGTTTAACCAGCCCAA CTGTGCCATCCCTGCCTCCACTTCAGAGGGATGGAGAAAGAAGTGGAGAAGGTCCTTAACCTGCAGTTTC TGCCTTTAAGCCAAAGAAACAAGCTGTGCGGACCTGGCCCATTAAGAGGCCTCAGTGGGAGAAGGGCTAA AAAGAGCCTGAGGTCATGCGGTTGGACACTGAACTTGGTAAAGACGTCCTTCCCCAAGGAAGCCATAATG TGCAGATGAACTGACCGAAGGAAAAGCTTGAGACAGCTTCCTGCGGGGGAGCCAGGTACAGAAGAGGCAG CTTGGGCTGACCCAGCATCTCTGCAAGATTTCCACCTGCTGGGCTGCCAGAGAGGTTTGTAGCCCGGCCC TGACTGCATCCTCCCCATCCCTGGGGCAACATTCCCTGGAGCTGTGCCAACAGGAGGACTGAAGCAGCCT GGTTTTAGAGTGTAGCTGTAAGGGCAGTGCCCGTGTGTACAGTCTGTGGAGTTTAGCTTAACGGGCAGGG CCCACATGTACAGTGTCTGTATATAAATGTGCTGTATCTGTTCATTTCTATGACTGCAGGGTTTTTTTAT ACAGTGTTTTTGGATTCATAATAAATTGATGGTTTTTTATGGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484518 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484518 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11174 same embryo
	EMAGE:11175 same embryo
	EMAGE:11173 same embryo
	EMAGE:11170 same embryo
	EMAGE:11171 same embryo
	EurExpress:euxassay_003508 same experiment
	MGI:4824485 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source