EMAGE mouse gene expression spatial database

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EMAGE:11174

BC002059 cDNA sequence BC002059 ( MGI:2384864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003503. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003503_01	euxassay_003503_02	euxassay_003503_03	euxassay_003503_04

euxassay_003503_05	euxassay_003503_06	euxassay_003503_07	euxassay_003503_08	euxassay_003503_09

euxassay_003503_10	euxassay_003503_11	euxassay_003503_12	euxassay_003503_13	euxassay_003503_14

euxassay_003503_15	euxassay_003503_16	euxassay_003503_17	euxassay_003503_18	euxassay_003503_19

euxassay_003503_20	euxassay_003503_21	euxassay_003503_22	euxassay_003503_23	euxassay_003503_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11174_wholemount_strong.wlz

11174_wholemount_moderate.wlz

11174_wholemount_weak.wlz

11174_wholemount_possible.wlz

11174_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11174_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

aorta

strong

regional

strong expression: see section 13 14 15 16 17

esophagus

strong

regional

strong expression: see section 15 16

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 moderate expression: see section 11 12 13

hindgut

moderate

regional

moderate expression: see section 16 17

rectum

moderate

regional

moderate expression: see section 16 17

midgut

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21

midgut loop

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22

urinary system

moderate

regional

moderate expression: see section 08 09

bladder

moderate

regional

moderate expression: see section 13 14 15 16 17 18

trachea

strong

regional

strong expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5958
Entity Detected:	BC002059, cDNA sequence BC002059 ( MGI:2384864)
Sequence:	sense strand is shown >T5958 GGACCTGCCTGTCATAAGAGGAGGGTGTTTCTTCCGGAATCCTTTTCGCTGGTTCCCTGTAATTAAGCAG GCTTAAGTGATTTGTCCTGTGTGCTGCTGGGTACTGTGAAGGAAACTCAGGCAAGTTCTGAAAGTGCGAC ATGATGAGGATATTGTCACTTCCCACAGAGGCTATAACTGGGAAGATCACAATATTGAGAAAGATTGTGA AAATTCTGGAAGTCACAGAAGGCATCTTCAAAGAGATGAACCAATTAATATTGGAGAGAAACCCTATGAC TGTGACCAATGTGGCAAAGCCTTTGCACATCGTAAACATCTTCAAATGCATAAGGGAACAACACATAATG GAGAGAAACCCTATGCATGTAATCAATGTGGTAAGGCCTTTACATGTCCCAGCTATCTTCAAATCCATAA AAGAAACCATACTGGAGAGAAACCCTATGAATGTGACCAATGTGGCAAAGCCTATGCACGACACAGTTAC CTTCAAACACATAAAAGAACACATACTGGAGAGAAACCCTATGAATGTAACCAATGTGGCAAAGCCTATG CACGACACAGTGGCCTTCAAACTCATAAAAGAACACATACTGGAGAGAAACCCTATGAATGTGACCAATG TGGCAAAGCCTTTGCATGTCACGGCTATCTTCAAGAACATAAAAGAACACACACTGGAGAGAAACCCTAT GAATGTAGCCAATGTGGCAAAGCCTTTGCATGTCACAGTACTCTTCGAAAGCATAAAAGATCACATACTG GAGAGAAACCTTATGAATGTCACCAATGTGGCAAAGCCTATGCACAACACAGTTACCTTCAAGCACATAA AAGAACACATACTGGAGAGAAACTCTATGAATGTCACCAATGTGGCAAAGCCTTTGCACATCGCAACCAT CTTCAAATACATAAAAAAACACATACTGGAGAGAAACTCTATGTATGTAATCAATGTGGCAAGGCCTTTG CTTGTCACAGCTATCTTCAAAAACATACAAGAAGACATACTGGAGAGAAACCCCTATGAATGTACCCAAT GCGGCAAAGCCTTTGCACAGTTTGGACGTCTTCAAAGTCATAAAAAAAAGACATACTGGAGAGAAACCCT ATGAGTGTAACCAATGTGGGTCAAGCCTTTGCAAAATATTGTCATCTTCAAAATCATAAAAGTTCACATA CTGAAGAGACACCCTGATGATGCAGCAGATGTGGTTAATGGCTTTGCATGTTATGGTGGTCTTAAAAAGT ATTTTAAAAACACATTCTTGAGAGAATCCTATAAATGTAATCAATGTAATATAGCCATTTGTTAACATTG ACCTTCTAAGGCATGAAACATAGTTGTGAGATTTTAGTGGGTGGGTGTGTCTGATTCCTTTGCCTGCTTG AGATGCTTTTTCTTCTATTTAATTGCTTTGCTAACTTTGATATGAGAGTTTGTACCTATTTTTATTAAAA TATGATATTCCTTGTTCATTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484516 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484516 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11172 same embryo
	EMAGE:11175 same embryo
	EMAGE:11173 same embryo
	EMAGE:11170 same embryo
	EMAGE:11171 same embryo
	EurExpress:euxassay_003503 same experiment
	MGI:4823401 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source