EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11182

Mrto4 MRT4, mRNA turnover 4, homolog (S. cerevisiae) ( MGI:1917152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003590. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003590_01	euxassay_003590_02	euxassay_003590_03	euxassay_003590_04

euxassay_003590_05	euxassay_003590_06	euxassay_003590_07	euxassay_003590_08	euxassay_003590_09

euxassay_003590_10	euxassay_003590_11	euxassay_003590_12	euxassay_003590_13	euxassay_003590_14

euxassay_003590_15	euxassay_003590_16	euxassay_003590_17	euxassay_003590_18	euxassay_003590_19

euxassay_003590_20	euxassay_003590_21	euxassay_003590_22	euxassay_003590_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11182_wholemount_strong.wlz

11182_wholemount_moderate.wlz

11182_wholemount_weak.wlz

11182_wholemount_possible.wlz

11182_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11182_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 17 18 19

pancreas

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 10 11 14 15 16

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 weak expression: see section 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6630
Entity Detected:	Mrto4, MRT4, mRNA turnover 4, homolog (S. cerevisiae) ( MGI:1917152)
Sequence:	sense strand is shown >T6630 CGATGCCCAAATCCAAGCGAGACAAGAAAGTTTCTTTAACAAAAACTGCCAAGAAAGGCTTGGAACTGAA GCAGAACCTGATAGAAGAGCTTCGGAAATGTGTGGACACCTACAAGTACCTTTTCATCTTCTCTGTGGCC AACATGAGGAACAGCAAGCTGAAAGACATCCGGAACGCCTGGAAACACAGCCGGATGTTCTTTGGCAAAA ACAAGGTGATGATGGTGGCCTTGGGTCGAAGCCCATCTGATGAATACAAAGACAACCTACATCAGGTCAG CAAGAAGTTGAGAGGTGAAGTTGGGCTGCTGTTTACCAACCGCACGAAGGAGGAGGTGAATGAGTGGTTC ACCAAGTATACAGAAATGGATTTTGCTCGAGCGGGGAACAAAGCAACTCTAACTGTGAGCCTGGATCCAG GGCCCCTGAAGCAATTCCCTCACTCCATGGAGCCACAGCTGAGGCAGCTGGGGCTGCCCACTGCCCTCAA GAAAGGTGTGGTGACCCTGCTGTCTGACTATGAAGTATGCAAAGAGGGTGACGTGCTGACCCCAGAGCAG GCCCGTATCCTGCTGTTTGGGTATGAGATGGCTGAATTCAAAGTGATCATCAAATACATGTGGGACGCCC AGTCAGGACGATTCCAGCAGATGGACGATGACCTGCCTGAGAGCGCGCCTGAGTCTGAGGGAGAGTCTGA GGAGGAGGATGACAGCTGACTCTGAGAGCCAGGACTATGGCCTTCCAGCGAGTTCTTCATCTTTGCTGGA CACCCCACCCCACCTCACCCTGCCCCCGGACTGCTGTCACCACTCTAGAGGGAACAGCTATTTATTTTGC TATGGACAAAGACAAGGGAGGGTGCTAGGGAATGATTTTGTTTCTATAAAGAATAAAATTGTGTCCGCGG GGTGTTCCCGGGAGACAGCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3598883 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3598883 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11186 same embryo
	EMAGE:11181 same embryo
	EMAGE:11185 same embryo
	EMAGE:11184 same embryo
	EMAGE:11183 same embryo
	EurExpress:euxassay_003590 same experiment
	MGI:4826460 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source