EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11184

Tmem9 transmembrane protein 9 ( MGI:1913491)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003611_01	euxassay_003611_02	euxassay_003611_03	euxassay_003611_04

euxassay_003611_05	euxassay_003611_06	euxassay_003611_07	euxassay_003611_08	euxassay_003611_09

euxassay_003611_10	euxassay_003611_11	euxassay_003611_12	euxassay_003611_13	euxassay_003611_14

euxassay_003611_15	euxassay_003611_16	euxassay_003611_17	euxassay_003611_18	euxassay_003611_19

euxassay_003611_20	euxassay_003611_21	euxassay_003611_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11184_wholemount_strong.wlz

11184_wholemount_moderate.wlz

11184_wholemount_weak.wlz

11184_wholemount_possible.wlz

11184_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11184_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18 19

4th ventricle lateral recess

moderate

regional

moderate expression: see section 05 06 07 08 17 18 19 20 21

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

lower jaw molar

moderate

regional

moderate expression: see section 06 07 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 16

upper jaw molar

moderate

regional

moderate expression: see section 06 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6590
Entity Detected:	Tmem9, transmembrane protein 9 ( MGI:1913491)
Sequence:	sense strand is shown >T6590 GCCTGCTGATCAGTGGCGGCTGCGGCTGAGCTTGCAGGCATCTAGTCTTGCTGGCTCAGCAAGCCCGATA AGCATGAAGCTGCTGTGTTTGGTGGCTGTGGTGGGGTGCTTGCTGGTGCCCCCAGCTCAAGCCAACAAGA GCTCTGAAGATATCCGGTGCAAATGCATCTGTCCGCCTTACAGAAACATCAGCGGGCACATTTACAACCA GAATGTGTCTCAGAAGGACTGCAACTGCCTGCATGTGGTGGAGCCCATGCCAGTGCCTGGCCACGATGTG GAAGCCTACTGCCTGCTCTGCGAGTGTAGGTACGAGGAGCGTAGCACCACAACCATCAAGGTCATTATTG TCATCTACCTGTCTGTGGTGGGGGCCCTCTTACTCTACATGGCCTTCCTGATGCTGGTGGACCCGCTCAT CCGGAAGCCAGATGCCTATACTGAGCAGCTGCACAATGAAGAGGAGAATGAGGATGCTCGCACCATGGCA ACAGCCGCTGCGTCCATTGGAGGACCCCGGGCAAACACTGTCCTGGAGCGGGTGGAAGGCGCTCAGCAGC GGTGGAAGCTGCAGGTGCAGGAGCAGCGGAAGACGGTCTTCGACCGACACAAGATGCTCAGTTAGATGGT TGCCATGATTGCATCAGAGACCTGGGCCATGGCTACCAGCTTCTGGGGCTCACTGCAGTCTTCCCTGGGT CTTCCCTTCAAATGCCCATGGCGTTTATCCTTCTCCCTCTCTAGAAATGTACTCGACTTTATAACGAGGG AGTGTGATTGGGTCTCTGTAGGTCTCTGGGGGGTAGAGGGGAGGGGAGGGAAGGCAGAAGGGAACAGAGA CATTTGAGGTGGCCACATGATTGGGTGGAATTCATCCCTCCTGTCTTCACCATTCCTCCCAGCTCCACAT CTTAAGGATGCTTACGGGAGACGAAGCTGTGTCATCAAGAGCTCAGTGGGTGCGAGGAAAGTATGATCCA GCGCTCAGCCTTCGCTCTAGGATGCTGTGGTCCCCATTCCCAGTTCCTCCAGTGCCAGTACTTTAGCTTG GCCTAGCCCCAGTCTCAGGAACTGTTGTGGTGCCCCTGAGCCCACAGTCATCTCCAGAGTCCACCTGGAA GCCTGTTCCCCTCTCCTCGGCTCCTGGTCCACCAGTGCATGGCAGTGCCCATGCATGCCGGCATATTCAG CAGCTGTCACCTTACTCCCATCCCAGGAGGCCGTAAGGCCTCCCACCTCTCCCCTGTGACTGCAGCTGCT GAGCCATAAAGTTGGACCATATGACACAAGGCCAATGGGGACCGGAGTACCATGGCTCCTGTCCTTGGAT GGTCTCTTGTCCCTGAATTTCATTGTATCATGCATGGAGAGAAATGTTTGTCTTTCTGTTGTCTATACAT CAAAGAAGCCATTAAATTGTTTTATTTCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3594475 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3594475 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11182 same embryo
	EMAGE:11186 same embryo
	EMAGE:11181 same embryo
	EMAGE:11185 same embryo
	EMAGE:11183 same embryo
	EurExpress:euxassay_003611 same experiment
	MGI:4828821 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source