Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11185

Lyl1 lymphoblastomic leukemia 1 ( MGI:96891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185
"Pseudo-wholemount" of euxassay_003603. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003603_01 euxassay_003603_02 euxassay_003603_03 euxassay_003603_04
EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185
euxassay_003603_05 euxassay_003603_06 euxassay_003603_07 euxassay_003603_08 euxassay_003603_09
EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185
euxassay_003603_10 euxassay_003603_11 euxassay_003603_12 euxassay_003603_13 euxassay_003603_14
EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185
euxassay_003603_15 euxassay_003603_16 euxassay_003603_17 euxassay_003603_18 euxassay_003603_19
EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185 EMAGE:11185
euxassay_003603_20 euxassay_003603_21 euxassay_003603_22 euxassay_003603_23 euxassay_003603_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11185Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11185_wholemount_strong.wlz
11185_wholemount_moderate.wlz
11185_wholemount_weak.wlz
11185_wholemount_possible.wlz
11185_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11185_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
heart
moderate moderate
spottedmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6629
Entity Detected:Lyl1, lymphoblastomic leukemia 1 ( MGI:96891)
Sequence:sense strand is shown

>T6629
CCGGGAGGTGGCGCAGCCCAGCTCTGAGGCCCAACGGGAGGGATTACCTGGAGCACACCGGCCGAACTGG
ACAGTGGAAACTGTCTCCTGACCTGGACTGACAAACCTGACCACACAGCCGGGTTCTCCAAGCTGTGGAA
ACTGCTACAGGGTTAGCATTCTATCCGTGGGGTCCATGTGCCCGCCCCAGGCCGGAGCAGAGGTGGGTTC
CGCCATGACTGAGAAAACTGAGATGGTATGTGCCTCCAGCCCAGCACCTGCCCCTCCCTCTAAGCTGGCC
TCACCTGGGCCCCTTTCTACAGAGGAGGTGGACCACCGAAACACGTGCACTCCCTGGTTGCCTCCCGGCG
TGCCAGTGATAAACCTGGGACACACCAGGCCCATAGGGGCAGCCATGCCCACCACAGAGCTCAGTGCCTT
TCGGCCCTCCTTGCTGCAGCTAACTGCCTTGGGAAGAGCTCCACCTACCCTGGCTGTACATTACCACCCT
CACCCCTTCCTCAACAGTGTCTACATTGGGCCAGCAGGACCCTTCAGCATCTTCCCTAACAGCCGGCTGA
AGCGCAGACCAAGCCATAGTGAGCTGGACTTGGCTGACGGGCACCAACCCCAGAAGGTGGCTCGGCGAGT
GTTCACCAACAGCCGTGAGCGCTGGCGGCAGCAGCACGTTAACGGCGCCTTCGCAGAGCTCAGGAAGCTG
CTGCCCACCCACCCGCCCGACCGGAAGCTGAGCAAGAACGAGGTGCTGCGCCTGGCCATGAAGTATATAG
GCTTCCTGGTGCGGCTGCTGCGAGACCAAACGGCTGTGCTGACCTCCGGCCCCAGCGCTCCCGGGTCCCG
CAAGCCACCTGCGCGCAGGGGCGTGGAGGGCAGCGCACGCTTCGGGGCCGAGCACAGAGTAGAGGCTGCA
CGCTCACAGCCCGTGCTTCCTGGGGACTGCGATGGCGACCCCAATGGGTCAGTGAGACCCATCAAGTTGG
AGCAGACATCCCTGAGTCCTGAGGTGCGGTGACCCAAGGCAGAGGCGCCTCACCTGCTGCTCAGTGAACT
CCCTGTAAAAGGATCTCAGGTCGCCCAGATGAGGAAACGCCCTGTAGCTCTGGAAGGGTGACCGGCGACG
CCAGGGATCCCCAGACTTTTAAGAAAAATCTCCCACAGGCTACTTGCAGTGGCTTTGCCAGTGTCCTCTT
GCCAGGGCCGAGGCCAGAGAATCGACAAGGCCAAGTAGCCATGGGATCCCAGAGGTCCTGAGGGAGGTAG
GGAAGGGTCTGGAAGCCTCGCGCTTCTCATCGGCGCCCCCTGCTGGAGGCGGGAAGACCCTAGACAAGTG
TGTGGGAGGACCTACCCCATGAAGCCCAACTGGTTGCGCTTGATCCATGATCCTGTTAACCGCGACAACG
AAGACTCAGGCACTCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3598911 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3598911 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11182 same embryo
 EMAGE:11186 same embryo
 EMAGE:11181 same embryo
 EMAGE:11184 same embryo
 EMAGE:11183 same embryo
 EurExpress:euxassay_003603 same experiment
 MGI:4826035 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS