EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11188

Acat2 acetyl-Coenzyme A acetyltransferase 2 ( MGI:87871)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001232. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001232_01	euxassay_001232_02	euxassay_001232_03	euxassay_001232_04

euxassay_001232_05	euxassay_001232_06	euxassay_001232_07	euxassay_001232_08	euxassay_001232_09

euxassay_001232_10	euxassay_001232_11	euxassay_001232_12	euxassay_001232_13	euxassay_001232_14

euxassay_001232_15	euxassay_001232_16	euxassay_001232_17	euxassay_001232_18	euxassay_001232_19

euxassay_001232_20	euxassay_001232_21	euxassay_001232_22	euxassay_001232_23	euxassay_001232_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11188_wholemount_strong.wlz

11188_wholemount_moderate.wlz

11188_wholemount_weak.wlz

11188_wholemount_possible.wlz

11188_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11188_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24

foregut-midgut junction

weak

regional

weak expression: see section 16 17 18

vibrissa

weak

regional

weak expression: see section 05 06 07 08 09 21 22 23 24

olfactory cortex marginal layer

strong

homogeneous

strong expression: see section 12 13 16 17 18 moderate expression: see section 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 18 not examined expression: see section 10 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 20

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 16

cervical ganglion

strong

regional

strong expression: see section 17

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

stomach

weak

regional

weak expression: see section 02 03 05 06 07 08 10 11

hindgut

weak

regional

weak expression: see section 16 17

midgut

weak

regional

weak expression: see section 04 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 14 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 16 17

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

maturing glomerular tuft

weak

regional

weak expression: see section 08 09 10 11 18 19 20 21

chondrocranium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4212
Entity Detected:	Acat2, acetyl-Coenzyme A acetyltransferase 2 ( MGI:87871)
Sequence:	sense strand is shown >T4212 GGACAAGGCTGAGAGCTAGCAGAGACCAGAGCGACAAGATGAATGCGGGCTCAGACCCCGTGGTCATCGT CTCAGCGGCGCGCACCGCTATAGGCTCCTTCAATGGTGCCCTGTCCACCGTGCCTGTCCACGAAATGGGG ACAACTGTCATCAAAGAAGTCCTGCAGAGGGCCAAGGTGGCTCCAGAAGAGGTGTCCGAGGTCATATTTG GACATGTCTTGACGGCAGGTTGTGGGCAGAATCCTACTCGACAAGCCAGTGTGGGTGCAGGGATCCCCTA TTCGGTTCCAGCATGGAGCTGCCAGATGATCTGTGGCTCGGGCCTAAAAGCCGTGTGTCTTGCAGCCCAG TCCCTAGCCATGGGCGACTCCACCATTGTGGTAGCTGGAGGCATGGAGAATATGAGCAAGGCTCCTCACT TGACTCACCTGAGAACAGGAGTCAGGATGGGTGAGGTGCCACTGGCTGACAGCATACTCTGTGATGGTCT GACAGATGCCTTTCACAACTACCACATGGGCATCACAGCTGAAAACGTAGCCAAAAAATGGCAAGTGAGC AGAGAGGCCCAGGACAAGGTTGCAGTTCTGTCACAGAACAGGGCAGAGCATGCTCAGAAAGCTGGCCACT TTGACAAGGAGATTGTGCCAGTGCTGGTGTCTTCCAGAAAAGGTCTTACTGAAGTGAAAATCGATGAATT TCCCCGTCATGGGAGTAACCTTGAAGCCATGGGCAAGCTGAAACCTTACTTTCTTACTGATGGGACAGGA ACTGTCACCCCAGCGAACGCATCAGGAATGAACGATGGCGCTGCTGCTGTGGTCCTTATGAAGAAGACAG AAGCTGAGAGGCGGATGCTGAAACCTTTAGCAAGAATAGTCTCCTGGTCCCAAGCCGGTGTGGAGCCTTC TGTTATGGGAGTAGGACCAATTCCAGCCATAAAGCAAGCTGTTGCAAAGGCAGGCTGGTCCCTGGAGGAT GTTGACCTGTTTGAAATCAATGAAGCCTTTGCAGCGGTGTCTGCGGCAATAGCTAAAGAACTTGGATTAA ACCCCGAGAAGGTCAACATCGATGGAGGAGCCATTGCCTTGGGCCATCCTCTGGGAGCATCTGGCTGCAG GATTCTAGTGACCTTGCTGCACACCCTGGAGAGAGTGGGCGGGACCCGTGGTGTTGCAGCCCTGTGCATT GGGGGTGGGATGGGAGTCGCAATGTGTGTTCAGAGAGGGTGAACTGCATGACAGCCACCACCTTGGACAG TTCTTGTTAAATCAGTGCAACACTAAGCAGAAAGGGAAACCAGAGGACCAAACTGAGGACAGGAACCCAG GTGGACAGCTTGCTGTACTTTAATGTGAGACACCCAAGGCTACGGCATTGCACCTGACACTGTTATAAAT AAGAGGGAAATCCAATCAGTCATCAAGGGCTCCAGAGTGAACAGCATTTTCATAACTTCCATGTTTATCG TCTTTACTTTCTGGGTGTAATTTTCTCTGATCATTGTTTTTTGTTTTTTTTGTTTTTCTTTGTTTTTGAG TTAGGGTCTCACCATATAGCCTCAAAATCGTTTTGTAGATCAGGTTGACTTTGAACTCCCGGAGATCCAC CTTTCTGTGCCTCCTGAATACTTGAATTAAAGGCATGCACCATTATGCAGGGCCCCCAAAATGATCCATT CAAAACATGGGGATTCGGCTTCTGTTACGAACTTCAGGCTCTTCACTTTAACTATGGTTGGTTCAAACGC CCCTGCGTGCCTGTCTCTCGGTTTCCCATTGATCAAATCAAGACCAATCCTGTAGTGAAAGTCGGATTCA GTGACCTCTTCCAGGCTGAGGTGGGGTAGGTATTCCAGCTGTTCTAGTCAGAAGACTGGCAGTGCCAACA AGTGTGCCTCTGGATTGTTGAAAGGTGGGCAGCAAGCAGCATCCACGTACTTATTTCTCTGCTTGACGGG ATGGGATGTTACCTGCTTCAGATATCTACCTTGACTTCCCCAAAATGATGACTGGAACTGGAACTGACAG TCAAATAAACTCTTTCCTCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4500929 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4500929 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11191 same embryo
	EMAGE:11187 same embryo
	EMAGE:11189 same embryo
	EMAGE:11190 same embryo
	EurExpress:euxassay_001232 same experiment
	MGI:4822907 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source