EMAGE mouse gene expression spatial database

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EMAGE:11189

Tubg2 tubulin, gamma 2 ( MGI:2144208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001259. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001259_01	euxassay_001259_02	euxassay_001259_03	euxassay_001259_04

euxassay_001259_05	euxassay_001259_06	euxassay_001259_07	euxassay_001259_08	euxassay_001259_09

euxassay_001259_10	euxassay_001259_11	euxassay_001259_12	euxassay_001259_13	euxassay_001259_14

euxassay_001259_15	euxassay_001259_16	euxassay_001259_17	euxassay_001259_18	euxassay_001259_19

euxassay_001259_20	euxassay_001259_21	euxassay_001259_22	euxassay_001259_23	euxassay_001259_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11189_wholemount_strong.wlz

11189_wholemount_moderate.wlz

11189_wholemount_weak.wlz

11189_wholemount_possible.wlz

11189_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11189_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 06 07 08 21 22 weak expression: see section 05

telencephalon marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23 24 weak expression: see section 02 03

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23 24 weak expression: see section 02 03

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 weak expression: see section 09 10 11 12 15 16 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

lower jaw molar

weak

regional

weak expression: see section 07 08 20

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 16 17

upper jaw molar

weak

regional

weak expression: see section 07 08 09 20

tail dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4213
Entity Detected:	Tubg2, tubulin, gamma 2 ( MGI:2144208)
Sequence:	sense strand is shown >T4213 CAGAGAGAGGCTATGAGAGAAAATGATGCCCAGGATGGGCTCTGCCGCCCGCCGGCTGCGGAGAGGCCCA CACTGCACACGCGCAGACCCAGCGCCCGCGTCAAAACATCAGTCGACAGCGCGTTCCCCATGTCCTGCGC CTCCGGCTGGCAGGAGTTCCTCTCAGTCGTGACCCCCGCCGCCAGGCCGGGGCGGACGAGCTAGGTCTGA TCGGCGATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAGTTCT GGAAACAGCTGTGTGCTGAACATGGAATCAGCCCCGAGGGCATCGTAGAGGAGTTCGCCACCGAGGGCAC TGACCGAAAGGATGTCTTTTTCTACCAGGCAGATGATGAGCACTACATCCCCCGGGCTGTGCTGCTGGAC CTGGAGCCCCGGGTGATCCATTCCATCCTCAACTCCTCCTATGCTAAGCTCTACAACCCGGAGAACATCT ACCTGTCGGAGCATGGCGGAGGAGCTGGCAACAACTGGGGCAGAGGATTCTCCCAGGGTGAGAAAATTCA CGAGGACATCTTTGACATTATAGACCGAGAAGCAGATGGAAGTGACAGTCTAGAGGGATTTGTGCTATGT CACTCCATTGCTGGGGGGACAGGTTCTGGTCTGGGCTCCTACCTCTTAGAGCGACTGAACGACAGGTACC CCAAGAAATTGGTGCAGACATACTCAGTGTTTCCCAACCAGGACGAGATGAGTGATGTAGTGGTCCAGCC CTACAACTCCCTCCTCACACTAAAGAGGCTGACCCAGAATGCGGACTGCGTGGTGGTGCTGGACAACACA GCCCTGAACCGGATCGCCACAGACCGCCTACACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTGG TGTCCACCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGATACATGAACAATGACCTCATCGG CCTCATCGCCTCGCTCATTCCCACCCCTCGGCTCCACTTCCTCATGACTGGCTACACCCCCCTCACCACG GACCAGTCAGTGGCCAGTGTGAGGAAGACAACAGTCCTGGATGTCATGAGGCGCCTGCTACAGCCCAAGA ATGTGATGGTGTCCACAGGTCGGGATCGTCAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCA GGGAGAGGTAGACCCCACCCAGGTCCACAAGAGCCTGCAGAGGATCCGGGAAAGGAAACTGGCCAACTTC ATTCCCTGGGGCCCAGCCAGCATCCAGGTGGCCCTGTCAAGGAAGTCTCCCTACCTGCCCTCAGCCCACC GGGTCAGCGGGCTCATGATGGCCAACCACACCAGTATCTCCTCGCTTTTTGAAAGTTCCTGCCAGCAGTA TGACAAACTGTGGAAGCGGGGGGCCTTCCTGGAACAGTTTCGCAAGGAGGACATCTTCAAGGACAACTTT GAGGAGATGCACAGATCGAGGGAGGTGGTGCAGGAACTCATTGACGAGTACCACGCGGCCACCCGGCCGG ACTACATCTCCTGGGGTACCCAGGAGCAGTGACTCTACTCTCTGCTGTCCCCTGGACAAGAAGCACAGTC CCCACCACGCCCAGACCCTCTGACCCAACTTCGCCTCACATTTTTGTGTTCATGTGCAATTTAGGAACTG TTCCTGTCTTCCTTTTCACCCCTGGCGCTTGGCTGGCTTGGTTAAGTTCTAATAAATAAAGCGTGATGAT GCATTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501003 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501003 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11191 same embryo
	EMAGE:11187 same embryo
	EMAGE:11188 same embryo
	EMAGE:11190 same embryo
	EurExpress:euxassay_001259 same experiment
	MGI:4829017 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source