EMAGE mouse gene expression spatial database

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EMAGE:11192

Ptbp1 polypyrimidine tract binding protein 1 ( MGI:97791)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003453. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003453_01	euxassay_003453_02	euxassay_003453_03	euxassay_003453_04

euxassay_003453_05	euxassay_003453_06	euxassay_003453_07	euxassay_003453_08	euxassay_003453_09

euxassay_003453_10	euxassay_003453_11	euxassay_003453_12	euxassay_003453_13	euxassay_003453_14

euxassay_003453_15	euxassay_003453_16	euxassay_003453_17	euxassay_003453_18	euxassay_003453_19

euxassay_003453_20	euxassay_003453_21	euxassay_003453_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11192_wholemount_strong.wlz

11192_wholemount_moderate.wlz

11192_wholemount_weak.wlz

11192_wholemount_possible.wlz

11192_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11192_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6680
Entity Detected:	Ptbp1, polypyrimidine tract binding protein 1 ( MGI:97791)
Sequence:	sense strand is shown >T6680 GTCTCCGCCATCTTGTGAGTCTATAACTCGGAGCCGTTGGGTCGGTTTCTGCTATTCCTGCGCCTCCGCT CCGTTCCCCGCGGGTCTCTTCCGTGTGCCATGGACGGCATCGTCCCAGACATAGCAGTCGGTACAAAGCG GGGATCCGACGAGCTCTTCTCCACGTGTGTCAGCAACGGCCCCTTCATCATGAGCAGCTCTGCCTCAGCA GCCAATGGAAACGATAGCAAGAAGTTCAAAGGTGACAACAGGAGCGCAGGAGTCCCTTCCAGAGTCATCC ATGTCAGAAAGCTGCCCAGCGATGTCACTGAGGGCGAGGTCATCTCCCTAGGGCTGCCCTTTGGAAAGGT TACCAACCTTCTCATGCTGAAGGGGAAGAACCAGGCCTTCATTGAGATGAACACAGAGGAGGCTGCCAAC ACTATGGTTAACTACTATACATCGGTGGCGCCAGTGCTTCGTGGACAGCCCATCTACATCCAGTTCTCCA ACCACAAAGAGCTCAAGACCGACAGCTCGCCCAACCAGGCACGTGCCCAGGCAGCCCTGCAGGCTGTAAA CTCCGTCCAGTCTGGAAACCTGGCCTTGGCAGCGTCCGCTGCTGCCGTGGATGCAGGAATGGCAATGGCA GGGCAGAGCCCAGTGCTCAGGATCATTGTGGAAAACCTTTTCTACCCAGTGACCCTGGACGTGCTGCACC AGATCTTCTCTAAGTTTGGCACCGTCCTGAAGATCATCACGTTCACCAAGAACAACCAGTTCCAGGCGCT GCTGCAGTATGCTGACCCTGTGAGCGCCCAGCATGCCAAGCTGTCCCTGGATGGCCAGAACATCTACAAC GCCTGCTGCACGCTGCGCATCGACTTCTCCAAGCTCACCAGTCTCAATGTCAAGTACAACAATGATAAGA GCAGAGACTACACTCGACCTGACCTGCCCTCTGGAGACAGCCAGCCTTCACTAGACCAGACCATGGCAGC AGCCTTTGGTGCGCCCGGCATAATGTCAGCCTCTCCGTATGCAGGAGCCGGGTTCCCTCCCACCTTTGCC ATCCCTCAGGCCGCAGGCCTCTCTGTCCCTAATGTCCATGGAGCCTTGGCCCCCCTGGCCATCCCGTCTG CTGCTGCTGCTGCTGCGGCCAGCCGCATTGCCATCCCAGGGTTGGCAGGTGCTGGGAATTCTGTCCTTTT GGTCAGCAATCTGAACCCTGAGAGAGTCACACCCCAAAGCCTCTTTATTCTCTTCGGCGTCTACGGTGAT GTGCAGCGGGTGAAGATCCTGTTCAATAAGAAGGAGAACGCACTTGTGCAGATGGCAGACGGCAGCCAGG CCCAGCTGGCCATGAGCCACCTGAACGGGCACAAGCTGCACGGGAAGTCAGTGCGCATTACACTGTCCAA GCATCAGAGTGTGCAGCTGCCTCGGGAGGGTCAGGAGGACCAGGGCCTGACCAAGGACTATGGCAGCTCC CCGCTGCACCGCTTCAAGAAACCAGGCTCCAAGAACTTCCAGAACATCTTTCCACCCTCAGCTACCCTGC ACCTCTCCAACATCCCGCCCTCTGTGTCAGAGGACGACCTCAAGAGCCTCTTCTCCAGCAACGGTGGTGT GGTCAAAGGCTTCAAGTTCTTCCAGAAGGACCGCAAGATGGCACTGATCCAGATGGGCTCTGTGGAGGAG GCTGTGCAGGCGCTGATTGAACTGCACAACCATGACCTGGGCGAGAACCACCACCTGCGAGTGTCCTTTT CCAAGTCCACCATCTAGGTGCCTGTGGGCCTGCATCAGCTTCCACCATTCCAGAAAAGCCACTTTAAAAA GCAGCTGAAGTGACCTTACAGACCAGAGATTTTATTTTTTTAAAGAGAGATCAGTTTACCTGTTTTTAAA AAAATTAAATCTAGCCCACTCTGCAGTGGCAGGAAATGGCTTGGACCACAGAGGCCCAGTCAATCCCAGC ACCATGCCTTGAGGGGCTCAGGCGTGAGGCCACAGCGGTGCCCCAAACATAGCCGCTTTCTGTGCCTTAC AAACCAGCGTGCCTCATGGCCGTACACCCACCCTCTAAGGCCACCAGTTGTCTCTAGACCTGCAGGTGAG CCAGGTGGCCTATACATAGGCTCCCCAATGCTGTGCAAGAGCTGGGGCCACTCCCCTACACCCTTATAAT CAAGTGACATGATTCTCCCATGTCCCTGGCCAGCCCGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTC ATTGTGACCTTTGTATTCTATTCCGTGTGGTCACAGACACCTGTGCCTAGCAATATTTCCACTTGACCAA ATACCCGAATCTTTTCCATTTATATGCAAGAGATATAGTTTTAAGTAACTTTTTATAGCAATGATACAAT TGTATGTGTGTATTCTGAGCTTCTATGTCCCCTGAGCTCCTTCCAGTGACCAACTTGCAGGTGGGCCCAG GCCAGGGCTGGAGAAAAAGCTCTGAGACTTGTGGGTTTCTACCTTCAAAATTTTGGACCAAAGTTTAGCT TTCTGCCCGCCTCTGACTGTGCCCAGTTGAGGGGCTGACCTCTAGGCCTTAATGTCCTCCTGCACAGCCA CCTCTATCCTAGCCTGTTCCTGTGGGAGGGGTTCCCTGCACCCTGGTTCTGTGGAAGGCGTGGAAGAGTG TAAATATTTATGATTTTATACCTGTGGGGCTGAGGCGGTGCAGCAGTTTTTTATGGTGACAGATGTATAT TTTGGTAACGACTACAGGCTCAGTATTGTCCTGGGGCCAGCCCTGGCCACATCTACCCCATCCCAGATGG TGTATTGGGGAAGGGGGGGGGGGCTTTCCATTAAAGCAGTTTTATAACCTCAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601527 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601527 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11194 same embryo
	EMAGE:11197 same embryo
	EMAGE:11193 same embryo
	EMAGE:11196 same embryo
	EMAGE:11195 same embryo
	EurExpress:euxassay_003453 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source