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EMAGE:11194

Gde1 glycerophosphodiester phosphodiesterase 1 ( MGI:1891827)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194
"Pseudo-wholemount" of euxassay_003384. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003384_01 euxassay_003384_02 euxassay_003384_03 euxassay_003384_04
EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194
euxassay_003384_05 euxassay_003384_06 euxassay_003384_07 euxassay_003384_08 euxassay_003384_09
EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194
euxassay_003384_10 euxassay_003384_11 euxassay_003384_12 euxassay_003384_13 euxassay_003384_14
EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194
euxassay_003384_15 euxassay_003384_16 euxassay_003384_17 euxassay_003384_18 euxassay_003384_19
EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194 EMAGE:11194
euxassay_003384_20 euxassay_003384_21 euxassay_003384_22 euxassay_003384_23 euxassay_003384_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11194Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11194_wholemount_strong.wlz
11194_wholemount_moderate.wlz
11194_wholemount_weak.wlz
11194_wholemount_possible.wlz
11194_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11194_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 19 20 21
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 06 16 17 18
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 07 16
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 07 16 17 18
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 08 09 15
cervical ganglion
moderate moderate
regionalmoderate expression: see section 07 08 15
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 13 14 15
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
not examined not examined
homogeneousnot examined expression: see section 02 03 04 24
neural retina
moderate moderate
regionalmoderate expression: see section 02 03 04 24 weak expression: see section 01
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 16 17 18 19 20
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 14
stomach
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09
hindgut
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18
rectum
moderate moderate
regionalmoderate expression: see section 15
midgut
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 21
midgut loop
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 12 13
bladder
moderate moderate
regionalmoderate expression: see section 14 15
urethra of male
weak weak
regionalweak expression: see section 14 15
left lung
strong strong
spottedstrong expression: see section 03 04 05 06 07 08 09 10
right lung
strong strong
spottedstrong expression: see section 11 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6681
Entity Detected:Gde1, glycerophosphodiester phosphodiesterase 1 ( MGI:1891827)
Sequence:sense strand is shown

>T6681
CGCCCGGCAGGTCCGGGGCTCCGGTCCGTGCCGGTGCAGGCGCCGGCATGTGGCTGTGGGAGGACCAGGG
CGGCCTCCTGGGCCCCTTCTCCTTCGTGCTGGTGCTGCTGCTGGTGGTGACACGCAGCCCCTTCAACGCC
TGCGTGCTCACCGGCAGCCTCTACATCCTGCTGCGGTTCTTCAGCTTCGAGCCGGTGCCGTCCCGCCGGG
CCCTGCAGGTGCTCAAGCCCCGTGACCGCGTGTCCGCCATCGCCCATCGCGGCGGCAGCCACGACGCGCC
CGAGAACACGCTGGCGGCCATCCGGCAGGCAGCTAAGAATGGAGCAACAGGTGTGGAGCTGGACATCGAG
TTTACTTCTGATGGAGTTCCTGTCTTGATGCACGATAACACAGTAGATAGGACAACAGACGGGTCTGGCC
GACTATGTGATCTGACATTTGAACAAGTTAGGAAACTTAACCCTGCAGCAAATCACAGACTCAGAAACGA
GTTCCCTGATGAAAGGATCCCTACCCTGAAGGAAGCTGTCACAGAGTGCCTCCGCCATAACCTCACCATC
TTCTTTGATGTCAAAGGCCATGCAGATATGGCTTCTGCTGCTCTAAAGAATATATACACGGAATTTCCAC
AGCTGTACAATAACAGTATGGTCTGCTCATTCTTGCCGGAAGTCATCTATAAAATGAGACAAACGGATCA
GAAGGTAATCACAGCCCTAACTCACAGGCCTTGGAGCCTGAGCCACACTGGAGATGGGAAGCCTCGCTAC
AGCGTCTTTTGGAAGCAGTCTGTGTTTGTGGTGTTGGACATCTTGCTGGACTGGAGCATGCACAATGTCT
TGTGGTACCTGTGTGGGATTTCAGCCTTCCTCATGCAGAAGGATTTTGTCTCCCCGGACTATTTGAAGAA
GTGGTCAGCTAAAGGAATCCAGGTTGTGAGTTGGACTGTCAATACCTTTGATGAAAAGAATTACTACGAG
TCCCACCTTGGGTCCAGCTACATCACTGACAGCATGCTAGAAGACTGTGCACCTCACTTCTAGACTCCCC
AGGAGGAAACACAGTTGCGAAACTGCCTGGAGGCCTCTTGCAGGGACATGAGAACACTCTGTTAGCACAA
GCCCTGGGGACCCTGTGGCTCATACTAGCAATAGTCACAGTGTCACCCCAACAAGCAGAAACCAGGCATG
GTGACCATGCTCCAAGGAAGGCTCCACACTTGCCCTTAAAATTCTGGATCTGCAAAAAGGCAGGGTGGCC
CCACCCTGACCCCGCTGAGGGTACAGAGCCACGAGGATGAGCAGAGTGGGTCCTGCAATTAGGGCGCAGG
CACCAATGCATGGGGCTTGCATGATCACTCCAAGTTGACATTTTAAATCTTGCCGTACTTCAGTAATTCA
CTTATTTCCAATATTTGTCGTCAGCTTTGTTAATGGCGACCTGTAGGTGTCAAACTTGTGACCACACTAA
TAAAATCATTGAAAGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3601420 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601420 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11197 same embryo
 EMAGE:11193 same embryo
 EMAGE:11196 same embryo
 EMAGE:11192 same embryo
 EMAGE:11195 same embryo
 EurExpress:euxassay_003384 same experiment
 MGI:4825031 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS