EMAGE mouse gene expression spatial database

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EMAGE:11195

Ctse cathepsin E ( MGI:107361)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003472. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003472_01	euxassay_003472_02	euxassay_003472_03	euxassay_003472_04

euxassay_003472_05	euxassay_003472_06	euxassay_003472_07	euxassay_003472_08	euxassay_003472_09

euxassay_003472_10	euxassay_003472_11	euxassay_003472_12	euxassay_003472_13	euxassay_003472_14

euxassay_003472_15	euxassay_003472_16	euxassay_003472_17	euxassay_003472_18	euxassay_003472_19

euxassay_003472_20	euxassay_003472_21	euxassay_003472_22	euxassay_003472_23	euxassay_003472_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11195_wholemount_strong.wlz

11195_wholemount_moderate.wlz

11195_wholemount_weak.wlz

11195_wholemount_possible.wlz

11195_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11195_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 17 18 19 20

thyroid gland

moderate

regional

moderate expression: see section 10 11 14 15

vibrissa

strong

regional

strong expression: see section 08 09 10 22 23 24 moderate expression: see section 07 11

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 09

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 11 12 13 14 15 16 17 18 19

not examined

regional

not examined expression: see section 01 02 03 04 05 23 24

neural retina

moderate

regional

moderate expression: see section 02 03 weak expression: see section 04 05 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 17 18 19 20 21 22

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 14 15 16 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 17 moderate expression: see section 12 13 14 18 19

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 10 20

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 15 18 19

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 10 20

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metanephros

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21 22

larynx

moderate

regional

moderate expression: see section 11 12 13 14

lung

strong

regional

strong expression: see section 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

not examined

regional

not examined expression: see section 06 07 08 09 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6678
Entity Detected:	Ctse, cathepsin E ( MGI:107361)
Sequence:	sense strand is shown >T6678 CAACTTCATTTGTTCCTCAGAACTGCAGATGCCCTGTGTGACTCCAGAAGCGGTTAGAAAGATCAGGCGT GGTTATCTCCATTTGCAGCTGAGGTGGCCAAGAGATAAGAGTTGCTTAAAGTCGGAGTTAGCAAACAACA AAACGGGGTTTCCACACAGAGCTCAGCCTCGGAGCCCAACCCATCCACTTACTACAAGAAAACGAAATTC TTGACATTTTGGGTTATATCCGGTAACTTCAAAGACTGGGCAGGGCAGGTCTGGGCACCAGGAAGAGCCA GTGCCCCTGCCCTGTGATCAAGAGGAAAGAAGGGTGGAAGGGAGATCGGAGCAGAGTGAGAGAGAAGCTA CCCCTTGGACCACAATGAAGCCCCTCCTTGTGCTGCTGCTGCTGCTGCTCCTGGATCTGGCTCAGGCCCA AGGTGCTCTGCACAGAGTGCCCCTCAGAAGACATCAGTCCCTTCGGAAGAAACTACGGGCCCAAGGACAG CTCTCAGAATTCTGGAGGTCTCATAACTTGGACATGACCCGACTCAGCGAGTCCTGTAATGTGTATTCGA GTGTCAATGAACCCCTCATCAACTACCTGGATATGGAATACTTTGGCACCATCTCCATCGGCACCCCGCC GCAGAACTTCACTGTCATCTTTGACACCGGTTCATCCAACCTCTGGGTCCCTTCTGTGTACTGCACCAGC CCAGCATGCAAGGCACACCCAGTATTCCATCCATCGCAGTCCGACACATACACGGAGGTAGGGAATCATT TCTCCATCCAGTATGGTACCGGGAGCCTGACAGGAATCATTGGAGCTGATCAAGTCTCTGTGGAAGGGTT GACTGTGGATGGCCAGCAGTTTGGAGAAAGTGTCAAGGAGCCAGGCCAGACCTTTGTGAATGCAGAGTTT GATGGGATTCTGGGTCTGGGATACCCCTCATTGGCTGCTGGAGGAGTGACCCCAGTGTTTGACAACATGA TGGCCCAGAACCTTGTGGCTCTGCCTATGTTTTCTGTCTACTTGAGCAGTGACCCTCAAGGTGGCTCAGG CAGTGAGCTGACTTTCGGAGGCTATGACCCCTCTCATTTCTCTGGGAGCCTCAACTGGATTCCAGTCACC AAGCAAGCCTATTGGCAGATTGCCTTGGATGGAATCCAGGTGGGAGACACTGTGATGTTCTGCTCCGAAG GCTGTCAGGCCATAGTGGACACAGGGACCTCTCTCATCACTGGCCCCCCCGACAAGATCAAACATCTTCA AGAGGCCATTGGGGCCACACCCATTGATGGAGAATATGCAGTGGATTGTGCCACTCTCGACACGATGCCA AACGTTACCTTCCTCATCAACGAGGTTTCATATACCCTCAACCCAACTGACTACATCCTGCCGGACTTGG TGGATGGAATGCAGTTCTGCGGCAGTGGCTTTCAAGGACTTGACATTCCACCTCCAGCTGGGCCCCTCTG GATCCTGGGGGATGTCTTCATCCGACAGTTCTACTCAGTCTTTGACCGTGGAAATAACCAAGTGGGATTG GCCCCCGCAGTTCCCTAAAGAGGGATGTATGCCTACATATGGATGCCTGATACCCATTTAACCTGTTAGA TACCTTTGTAACTATCAAAGCCGTCATTTCCCATGGGGTGTAGCCACCCCAGAGTATTCAGACCAATCAA AGCATAAGAGTGCACCCCACTCACTGCAAACACACACACACACACACCACCTCTACCATCACCACGATGG AAGAAGTTCTGTCTATAGTCTTCACTGCTTATTGTTGACTTTCTATTATGGAAATCTCTAAACATGTACA CAGTAGACGTGATGGCAAGATAAATACCCACACACCTCTGCCTCAGGTCACAACCCATCCATGTGTGGAC CAGACTCTCTATCTTCCATCCCTCTGGTTCCACGCCTCTAGATTTGGAAGCAGATTCTAAGCACCAGGTC ATTTTATCTAATGTCTAACATCCTTACAAATCAGAATTTAAATGCCTCACCCTCTCATAAATGTGGATCT GTTTTTTACAGTTGGTTTATTTGTATCAGGATTAAAACCAGATCCATAACTGGACAAAAAAACCCATAAA CTGATTTGATTTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601519 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601519 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11194 same embryo
	EMAGE:11197 same embryo
	EMAGE:11193 same embryo
	EMAGE:11196 same embryo
	EMAGE:11192 same embryo
	EurExpress:euxassay_003472 same experiment
	MGI:4824112 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source