EMAGE mouse gene expression spatial database

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EMAGE:11197

Pter phosphotriesterase related ( MGI:107372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003385. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003385_01	euxassay_003385_02	euxassay_003385_03	euxassay_003385_04

euxassay_003385_05	euxassay_003385_06	euxassay_003385_07	euxassay_003385_08	euxassay_003385_09

euxassay_003385_10	euxassay_003385_11	euxassay_003385_12	euxassay_003385_13	euxassay_003385_14

euxassay_003385_15	euxassay_003385_16	euxassay_003385_17	euxassay_003385_18	euxassay_003385_19

euxassay_003385_20	euxassay_003385_21	euxassay_003385_22	euxassay_003385_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11197_wholemount_strong.wlz

11197_wholemount_moderate.wlz

11197_wholemount_weak.wlz

11197_wholemount_possible.wlz

11197_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11197_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 weak expression: see section 13 14 16 22

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 10 11 moderate expression: see section 05 06 07 weak expression: see section 12 13 14

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 18 19 20 weak expression: see section 02 03 16 17

pons ventricular layer

strong

homogeneous

strong expression: see section 11 weak expression: see section 12

spinal cord ventricular layer

strong

regional

strong expression: see section 10

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 not examined expression: see section 14 15

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23

kidney calyx

strong

regional

strong expression: see section 08 09 10 19 20 moderate expression: see section 11 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6679
Entity Detected:	Pter, phosphotriesterase related ( MGI:107372)
Sequence:	sense strand is shown >T6679 CGGCGCACAGCGCGGAATGAAGGAAGCCCCGGAGCTGGTTAAGTAGCTGTGGACCTGGTAGGAAAGAGAA AATCCCTGAGACACTATCAGGAATGTCTTCCTTAAGTGGGAAAGTACAAACAGTTCTGGGCCTTGTAGAA CCCAGCCAACTGGGACGCACCCTGACCCACGAGCATCTGACAATGACCTTTGACAGTTTTTACTGCCCAC CTCCTCCATGCCACGAAGTCACCTCCAAGGAACCTATCATGATGAAAAATCTATTTTGGATTCAGAAAAA CCCCTATTCCCATCGAGAGAACCTTCAGTTGAATCAGGAGGTAGGAGCCATAAGAGAAGAGCTGTTGTAT TTCAAGGCTAAGGGCGGAGGAGCCTTGGTGGAGAATACGACAACTGGGCTCAGCAGGGACGTGCATACGC TGAAGTGGCTGGCAGAGCAGACTGGAGTCCACATCATAGCTGGAGCTGGGTTTTATGTTGATGCAACTCA CTCTGCAGCAACCAGAGCCATGTCAGTGGAGCAGCTTACAGATGTCCTTATTAATGAAATTCTCCATGGA GCTGATGGCACCAGCATCAAGTGTGGAGTTATTGGAGAAATTGGCTGCTCCTGGCCTTTGACTGACAGCG AGAGAAAGATACTTGAGGCTACAGCTCACGCCCAGGCTCAGCTTGGCTGTCCTGTCATCATCCATCCTGG ACGGAACCCAGGTGCACCATTCCAGATAATCCGTATACTGCAAGAAGCAGGAGCAGACATCTCCAAAACA GTCATGTCCCACCTTGACAGGACTATATTTGATAAGAAAGAGCTGCTGGAGTTTGCTCAACTTGGCTGCT ACTTGGAATACGATCTCTTTGGTACGGAACTCCTTAATTACCAGTTGAGCCCAGATATTGATATGCCTGA TGATAACAAAAGAATTAGAAGGGTCCATTTTCTAGTGGATGAGGGCTATGAAGATCGGATTCTCATGGCA CATGACATACACACAAAGCATCGGTTGATGAAGTACGGAGGTCACGGCTACTCACACATCCTTACCAACA TTGTTCCTAAGATGCTCCTTAGAGGTCTGACTGAGAGGGTGCTTGACAAGATACTCATAGAAAACCCTAA ACAATGGCTGACTTTTAAATAGGATGGCTGTTCACGAACCCAGACCTGGAGGATACAATGAGCAGAGAAT AGTTGGTGATTTCAAATCTACTGGAGACATTAATCCAGTCTACATAGAACTGGTGAATGGTCACTTCTCT CCTATGAGAAGCTGGATAACTACCACAGGGACATCTCTGGTGGGGGCCACAGGGCTCAAGTGAGTCCCAT TGTCTTTCCTTAATAAAATAAATATTGATAAAAGAGCATGTTTCCAAACAGTAGTTTAAAACTATATCCC CTAAGAATCATTTTGGATGTCTTCCCCAACCCTGACTCTGTGATCTGCACTACTTGAGAAAAATGAAAGT GTTTCTAGCTAAGTTGCCCCTTCTGGAGCAACCTAATGTTTCTTGTAATATTGATGATCCTACTAATTAT CCTGCTGTTCTTTAATTAATGCTTAATGAATAATATGGCATTTTAAAATCACTTTTGCAACAAGGGAAGT TAAATTTTGAGACATTTTTTCCCAAAGGAGACTGCAATAAAATTACCAATTCACAACAATAAAGAAATTT CGAAGGTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601675 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601675 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11194 same embryo
	EMAGE:11193 same embryo
	EMAGE:11196 same embryo
	EMAGE:11192 same embryo
	EMAGE:11195 same embryo
	EurExpress:euxassay_003385 same experiment
	MGI:4827502 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source