EMAGE mouse gene expression spatial database

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EMAGE:11198

Kctd6 potassium channel tetramerisation domain containing 6 ( MGI:1918643)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003600. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003600_01	euxassay_003600_02	euxassay_003600_03	euxassay_003600_04

euxassay_003600_05	euxassay_003600_06	euxassay_003600_07	euxassay_003600_08	euxassay_003600_09

euxassay_003600_10	euxassay_003600_11	euxassay_003600_12	euxassay_003600_13	euxassay_003600_14

euxassay_003600_15	euxassay_003600_16	euxassay_003600_17	euxassay_003600_18	euxassay_003600_19

euxassay_003600_20	euxassay_003600_21	euxassay_003600_22	euxassay_003600_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11198_wholemount_strong.wlz

11198_wholemount_moderate.wlz

11198_wholemount_weak.wlz

11198_wholemount_possible.wlz

11198_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11198_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 23 moderate expression: see section 07 08 09 21 22

thalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 moderate expression: see section 08 17 18

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 13 14 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 14 15 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6618
Entity Detected:	Kctd6, potassium channel tetramerisation domain containing 6 ( MGI:1918643)
Sequence:	sense strand is shown >T6618 GCGTCTCCCGGCCGGGTCGCCTCCGCGCTCGTGCAAGTTCCCTGGCTCCTGAGCTCTGAGGACGCAGTGC TGGAGCAGATGGATAATGGGGACTGGGGCTATATGATGAGTGACCCAGTCACATTAAATGTAGGTGGACA CTTGTACACGACATCGCTTACCACGTTGACACGCTACCCGGATTCTATGCTTGGAGCTATGTTTGGGGGT GACTTCCCCACAGCCCGAGACCCTCAAGGCAATTACTTCATTGATCGAGACGGACCGCTCTTCCGCTATG TCCTTAACTTCCTACGGACTTCAGAACTGACACTCCCCCTGGACTTTAAGGAGTTTGATCTGCTTCGGAA AGAGGCTGATTTCTACCAGATCGAACCCTTGATTCAGTGTCTCAATGACCCCAGGCCTCTGTATCCTATG GATACTTTTGAAGAAGTCGTAGAGCTGTCTAGCACTCGGAAGCTTTCTAAATATTCCAATCCGGTGGCCG TCATCATCACCCAGTTAACCATCACCACAAAGGTCCACTCCTTACTCGAAGGCATCTCAAACTATTTCAC CAAGTGGAATAAGCACATGATGGACACCAGAGACTGCCAGGTCTCCTTCACCTTTGGACCGTGCGATTAC CACCAGGAAGTCTCTCTCCGGGTCCATCTGATGGAGTACATCACGAAGCAAGGTTTCACGATCCGCAATA CTCGGGTGCATCACATGAGCGAGCGGGCCAATGAGAACACAGTAGAGCACAACTGGACTTTCTGTAGACT GGCCCGCAAGACGGACGACTGACTGCTGGCCAGCCCTCAGAGAACTTCCTGCAAGCTCACGTTTTGGGAC ATGGAAGAGACTGTTGTTGGGTTTTAGTGTGGGCCCTCTTTTTTTTTTTAATTTTTTGATTCAGTCTTTG TATTTATTTGAAGGCATTGAGGACCAGAAGGAAGTTTTGTGTGTGAGCAGTCTCCTCCCTGTTTTGTTCC CAAGTATGCATGTGCCTGTTCCGAGTCTCTAGAAACCTTTTTTATAAAAAGAGATCTGAAAATCATTATG GTATATAATCTACCCTTAACAGTGATAAAATGATTTCTGAGAACGTGGTTCCTGACTCGACTGGAAGGCT AAAAACAGGAATAAAAGATTAAAGAGGGTATTCATGATGTCTTTGAGAAAAATCAGAATATCATGTAGGG CGACCTAGTTTCCAGACCAGTAGGCAGTATTGTGGTGTTAAAGGAAGACTGGCCCAGTCATCTGAGGGTA CTTGTTAAGGACTGCTTTCTACAGTTTTGACAACTGTTTCTTTCTATGCATATAAATCAAGCAACCAAAT ATCTGTAGCCATGGAAATGTCTGACTAGAAATATTTATATTGGATTCTCAATACAAAATGTCCCTGTGGT AGAAACCTTATGCCTGGTACAGTTTCATTCCCAAGGTACTGTCTACCAGGAAAGAAAAATCTAATAAAAA ATGGAATATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3597940 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3597940 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11199 same embryo
	EurExpress:euxassay_003600 same experiment
	MGI:4825739 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source