EMAGE mouse gene expression spatial database

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EMAGE:11217

Hsd3b7 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 ( MGI:2141879)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003409. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003409_01	euxassay_003409_02	euxassay_003409_03	euxassay_003409_04

euxassay_003409_05	euxassay_003409_06	euxassay_003409_07	euxassay_003409_08	euxassay_003409_09

euxassay_003409_10	euxassay_003409_11	euxassay_003409_12	euxassay_003409_13	euxassay_003409_14

euxassay_003409_15	euxassay_003409_16	euxassay_003409_17	euxassay_003409_18	euxassay_003409_19

euxassay_003409_20	euxassay_003409_21	euxassay_003409_22	euxassay_003409_23	euxassay_003409_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11217_wholemount_strong.wlz

11217_wholemount_moderate.wlz

11217_wholemount_weak.wlz

11217_wholemount_possible.wlz

11217_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11217_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

gut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 16 17

heart

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

lower jaw molar

moderate

single cell

moderate expression: see section 05 06 07 08 10 11 12 16 17 18 19 20 21

upper jaw molar

moderate

single cell

moderate expression: see section 05 06 07 08 16 17 18 19 20 21

liver and biliary system

moderate

regional

moderate expression: see section 01

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 14 15

lung

weak

single cell

weak expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6611
Entity Detected:	Hsd3b7, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 ( MGI:2141879)
Sequence:	sense strand is shown >T6611 CAACCTCCTCCAGTGGCCAGTTTGTGCGCCCTGGGACTTACTACAGCTGGGCATGGCAGACTCTGCACAG GTGCCAACACTGGTATACCTGGTCACAGGTGGCTGCGGCTTCCTGGGGGAACATATTGTTCGGATGCTGC TGGAACGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAGTTCTTGGCTGGAGGAGCT GAAAGCAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCCCATGAGGTGGCAGCAGCC ATGTCTGGATCACATGTGGTCATCCATACAGCTGGGTTGGTGGATGTGTTTGGGAAGGCCAGTCCAAAGA CCATCCACAAAGTCAACGTGCAGGGCACACAGAATGTGATTGATGCTTGTGTGCAGACTGGCACTCAGTA CCTGGTCTACACGAGCAGCATGGAAGTGGTGGGGCCTAACATCAAGGGCCACCCCTTCTACAGGTGAGCT CAGCCCCACTTAATCTTAGAGCCCATTTCCCTCCAGCACTCAGTCTTCTTTCTCCCCTCTCCAGGGGCAA TGAAGATACCCCATATGAGGCAGTCCACAGCCATCCCTACCCATGCAGTAAAGCCCTTGCTGAGCAGCTG GTCCTCGAGGCCAATGGAAGGAAGGTCAATGGAGGGCTACCCCTGGTGACATGTGCCCTTCGACCCACGG GCATTTATGGTGAAGGTCATCAGGTCATGAGAGACTTCTACTACCAGGGACTGCGCTTTGGAGGTCGTCT ATTTCGGGCCGTCCCAGCTTCTGTGGAGCACGGTCGGGTCTATGTTGGTAAGGACAGGGACAGCAGGGCT CGAGGAGTGGCTCTGATGGTGGCAGAGTCACGCGTGTACGCCTGGCTCCACGCGAGCTCAGGCAGACAGT GGCATGTGGGCATTTTCTGCATGAGTTGAAAAACGGGGAAAGCATCCAAGCAGTTGGGAGGGCTGACATA AGAACATGGCGTAGGTTATGATGAGCAACAGGAGCGACAGCTCTCCAGAGAAAGGGAGAAAGCATCTACA CGGGATCCAAGTAGCTGCAGGATGCCCAGGAGGGCTGGTGGCTACGGGGCCTTGGAGAAGGGCAACTTTG ATTAATCAGACAGTAGAGAGGTGTGGGCAAGATCTACACGGGGCCAAGGCCAGCTTCTCTCCCTCCCTCC CTCCCTGCTGGCAGGCAATGTTGCTTGGATGCACATACTGGTGGCCCGGGAGCTGGAGCAGCGGGCAGCA CTCATGGGTGGCCAGGTGTATTTCTGCTATGATAAGTCACCTTATAAAAGCTACGAGGACTTCAACATGG AGTTTCTGAGTCCCTGTGGTCTTCGACTGATAGGCGCCCACCCACTGCTGCCCTACTGGCTGCTAGTGCT GCTGGCTACCCTCAATGCCCTGCTGCAGTGGCTGCTCCGCCCACTGGTGCTGTACACACCCCTGCTGAAT CCCTACACGCTGGCTATGGCCAACACCACCTTTACTGTCAGTACCAACAAGGCACAGCGGCATTTTGGCT ACAAGCCCCTCTTCTCATGGGAAGAGAGCAGGACCCGCACCATTCAGTGGGTGCAGGCGATGGAGGGTTC AGCTCGGTGATGGCAGGGCCAGGAACTGGAGACCACTGTGGCACATACTCCAGGTCCTGAGCCCCCAAAT CTGGATGAAGAGAAATGGCTGCCTTTTGAAGATGAAGACTCTGCTCTACACATCATGACTCCGTGATGTG GACCCTGCCACATCTTAACTACACAGATCCTGAGACTGGTTCATATTCCAGTCTCCTAGTTCTAGGATAG GGTTTGGGGAAAGGCCTATCTTTGGTGCCAACTCAGGCCTGCCAGGTAGTTGGCAAAACTGATTTCTTGA ATGGTCTCACTACCTCTTCCTGTTTCCAGTTTCTCAAGCAGGGAGGAACAGAGACTCCAGAGATCTTACG CTGGTCTGCTTACTTCAGCTACCATTTTCAGACCTGGCTCCTGCCACATCTGGCTCTCCTTAAGGGGTTA TAGTTTCACCATTTTTTAAGTGTATCTCTTTTAGACATTTATCTTTTAATTTGCTTTAAAGAAATCTGAA GAAATCAATGATTTCCCATGTCTTGCCTCTCCTAACAAATTCACCTATGACATTGTTAGCTTCCTCCCCT AGGATGCCAACCTGTATCTGGCCAAGCCTAGAATAAAATCCTTTTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3596935 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3596935 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11218 same embryo
	EMAGE:11215 same embryo
	EMAGE:11216 same embryo
	EurExpress:euxassay_003409 same experiment
	MGI:4825472 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source