EMAGE mouse gene expression spatial database

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EMAGE:11241

Cisd1 CDGSH iron sulfur domain 1 ( MGI:1261855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001270. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001270_01	euxassay_001270_02	euxassay_001270_03	euxassay_001270_04

euxassay_001270_05	euxassay_001270_06	euxassay_001270_07	euxassay_001270_08	euxassay_001270_09

euxassay_001270_10	euxassay_001270_11	euxassay_001270_12	euxassay_001270_13	euxassay_001270_14

euxassay_001270_15	euxassay_001270_16	euxassay_001270_17	euxassay_001270_18	euxassay_001270_19

euxassay_001270_20	euxassay_001270_21	euxassay_001270_22	euxassay_001270_23	euxassay_001270_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11241_wholemount_strong.wlz

11241_wholemount_moderate.wlz

11241_wholemount_weak.wlz

11241_wholemount_possible.wlz

11241_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11241_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 04 06 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 09 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 19 20

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

chondrocranium

weak

regional

weak expression: see section 01 02 03 04 05 06 07 20 21 22 23 24

tail dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4911
Entity Detected:	Cisd1, CDGSH iron sulfur domain 1 ( MGI:1261855)
Sequence:	sense strand is shown >T4911 CCCACGCGTCCGCTTGCCGCGGCGCCTGCGCAGTGGCAGTGAGTGGGCCCCGAGGTCGCGTCTTGCCCAA GTCTCCGCGGTCCCCAGCGCTCGCTCGCGCGGTCCTGCCACGGCCTTCCTGCTGCCCGCGCCATGGGCCT CAGCTCCAACTCCGCTGTGCGAGTTGAGTGGATCGCGGCCGTCACCTTTGCTGCTGGCACAGCCGCTCTC GGTTACCTGGCTTACAAGAAGTTCTACGCTAAAGAGAATCGCACCAAAGCTATGGTGAATCTTCAGATCC AGAAAGACAACCCGAAGGTGGTGCATGCCTTCGACATGGAGGATCTGGGGGATAAGGCCGTGTACTGCCG ATGCTGGAGGTCTAAAAAGTTCCCCTTCTGCGATGGGGCTCACATAAAGCACAACGAAGAGACTGGCGAC AACGTAGGACCTCTGATCATCAAGAAAAAGGAAACCTAATGGACAGTTGCGAGGCTGCACCCAGCGTGTT GTGATGTCACCTGCTGATTTACGTAGAATGGCACCCAACCCACCGTCTGATTGGCCTCCCCGGTTCTAGA TGTGGTTGGTCCCTGCAAATCACAGCTCTCATATCCATGGCATCGGCCTTGCTACTGAAACATGTGGTGC ACGTTTGTTGAAAGAAGAAGAAAGGCTAAACCAACCTCGTGCTATATGGGTTATTTTGGTCTTGTAAGGA TCCGTTCCTTTAAAATAATGGTCTTAGAATATAGTTGTATCTTGAGGTTAAAGTATTAAATTATTCCAAA ATCATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5102315 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102315 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11245 same embryo
	EMAGE:11244 same embryo
	EMAGE:11242 same embryo
	EMAGE:11240 same embryo
	EMAGE:11243 same embryo
	EurExpress:euxassay_001270 same experiment
	MGI:4823881 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source