EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11243

Sc5d sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) ( MGI:1353611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003227. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003227_01	euxassay_003227_02	euxassay_003227_03	euxassay_003227_04

euxassay_003227_05	euxassay_003227_06	euxassay_003227_07	euxassay_003227_08	euxassay_003227_09

euxassay_003227_10	euxassay_003227_11	euxassay_003227_12	euxassay_003227_13	euxassay_003227_14

euxassay_003227_15	euxassay_003227_16	euxassay_003227_17	euxassay_003227_18	euxassay_003227_19

euxassay_003227_20	euxassay_003227_21	euxassay_003227_22	euxassay_003227_23	euxassay_003227_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11243_wholemount_strong.wlz

11243_wholemount_moderate.wlz

11243_wholemount_weak.wlz

11243_wholemount_possible.wlz

11243_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11243_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 12 13 14 15 16

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

weak

regional

weak expression: see section 09 10 18

spinal cord

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 17

cervical ganglion

weak

regional

weak expression: see section 10 18

thoracic ganglion

weak

regional

weak expression: see section 14 15

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 17 18 19

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 12 13 16 17 20 21 weak expression: see section 10 11 14 15 18 19 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4908
Entity Detected:	Sc5d, sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) ( MGI:1353611)
Sequence:	sense strand is shown >T4908 CCCACGCGTCCGGCCGCGGCTGACGGAGCTGCTGGGCCTGGCAGGTTCAGTCCTCGCCCTTATCTGATGG TAGATCCTGCCTCCAAGTGACAGACACAACCATTGTTTATTGGGTCACATACCAGGGGCTGCGATGGACC TGGTTCTCAGTGCCGCCGATTACTACTTCTTCACTCCGTATGTATATCCAGCCACGTGGCCTGAGGACAA CATCATCCGACAAACTATTAGCCTCCTGATTGTCACAAACCTGGGTGCTTACATTCTCTACTTCTTCTGT GCAACCCTCAGCTATTATTTTGTCTATGATCATTCCTTAATGAAACACCCACAGTTTTTAAAGAACCAAG TCTCGCGTGAGATCGTGTTCACTGTCAAGTCTTTGCCTTGGATCAGCATCCCCACCGTCTCACTATTCCT GCTGGAGCTGAGGGGTTACAGCAAACTCTACGATGACATCGGAGACTTTCCAAATGGCTGGATTCATCTC ATGGTTAGCGTCGTATCCTTCCTCTTTTTCACAGACATGTTGATCTACTGGATTCATAGGGGCCTGCACC ACAGACTGGTCTACAAGCGCATACATAAACCACATCATATTTGGAAGATCCCCACGCCGTTTGCAAGTCA TGCTTTTCACCCTGTGGACGGCTTCCTTCAGAGTCTGCCTTACCATATATACCCCTTTGTCTTTCCACTG CACAAGGTGGTCTACTTAGGTTTATATGTCTTGGTTAATGTCTGGACAATTTCTATTCATGATGGTGATT TTCGGGTTCCCCAGATCTTAAGGCCATTTATTAACGGGTCAGCTCACCACACAGACCACCACATGTTCTT TGACTATAACTATGGACAGTATTTCACATTGTGGGATAGAATTGGAGGCTCTTTTAAACATCCTTCCTCT TTTGAAGGGAAAGGACCACATAGTTACGTGAAGAACATGACAGAAAAAGAATCTAACAGCTTTGCAGAAA ACGGCTGTAAAGGCAAAAAAGTAAGCAATGGAGAGTTTACAAAGAATAAGTAGATTATTGCCCAATTATT AACTATTTTAAATAAGAAAATAATCTACACAGTCAAGATACCTAGCAAATAAGTGGTGCCATTGGAATGT CAGCATTGCTTGAGTGTCATGCTGAGGGATGGTCCTGACAGTAAACAGCGGGAAGACACCAGGAGCATTG TAATCGCTTGGTTAATTGTCCGACATTGGTCCAGGAACAACTTGTCTTTTCAGCCGGCTGACCTGCAGCC TGTACAGCTCTGAAGCATCTTTAAATACGATAGAAAATAAGTTACTCAAGAAGCTGGTCTGTGTCCTTTT GCCTTAGTCCATGCATACTCATTAAGGAAAAAAAGTACCATTGTGCTAAACGCTACTGAGACTAACCAGG AAACCCTAAAGATGAAGATGTTCCTGTCCCCGGAGCGGCTGGCTCCACCGCAGTGCTGGGGAGTTGCAGG ACTTCATTCAAATAGGAATATCAGTCCAAGCAGGCTGATACGTGAGTGGCAGTGGATAGGATTTGCTTAA TGGCTGATGTAATAAGATTGTACTTCCTATACAGAGGCACCTTACTCTCTGGGGCATTATCAGATTTTAA TAAATGAATAAATAAATGGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5101986 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5101986 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11245 same embryo
	EMAGE:11241 same embryo
	EMAGE:11244 same embryo
	EMAGE:11242 same embryo
	EMAGE:11240 same embryo
	EurExpress:euxassay_003227 same experiment
	MGI:4827883 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source