EMAGE mouse gene expression spatial database

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EMAGE:11247

Rg9mtd1 RNA (guanine-9-) methyltransferase domain containing 1 ( MGI:1196261)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006517. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006517_01	euxassay_006517_02	euxassay_006517_03	euxassay_006517_04

euxassay_006517_05	euxassay_006517_06	euxassay_006517_07	euxassay_006517_08	euxassay_006517_09

euxassay_006517_10	euxassay_006517_11	euxassay_006517_12	euxassay_006517_13	euxassay_006517_14

euxassay_006517_15	euxassay_006517_16	euxassay_006517_17	euxassay_006517_18	euxassay_006517_19

euxassay_006517_20	euxassay_006517_21	euxassay_006517_22	euxassay_006517_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11247_wholemount_strong.wlz

11247_wholemount_moderate.wlz

11247_wholemount_weak.wlz

11247_wholemount_possible.wlz

11247_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11247_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 2 phalanx

strong

regional

strong expression: see section 01 02 03

forelimb digit 3 phalanx

strong

regional

strong expression: see section 01 02 03

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 03 04 18 19 20 21 22

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 02 03 04 18 19 20 21 22

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 02 03 04 18 19 20 21 22

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 03 04 05 06 18 19 20 21 22

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 03 04 05 06 07 18 19 20 21 22

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 10 15 16 17 18

dermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 05 06 moderate expression: see section 03 04 07 20 21 22

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 moderate expression: see section 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

rest of cerebellum mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

pons ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 18 19 moderate expression: see section 16 17

tegmentum

strong

regional

strong expression: see section 07 08 09 10 11 12 14 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 15 16 17 18 19 20

spinal cord mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 moderate expression: see section 16

hyoid

strong

regional

strong expression: see section 12 13 14

lower jaw incisor

strong

regional

strong expression: see section 10 11 12 15

lower jaw molar

strong

regional

strong expression: see section 06 07 18

upper jaw incisor

strong

regional

strong expression: see section 11 12

upper jaw molar

strong

regional

strong expression: see section 06 07 18

kidney calyx

strong

regional

strong expression: see section 08 09 10 11 18 19 20

lung

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

temporal bone

strong

regional

strong expression: see section 02 03 04 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6806
Entity Detected:	Rg9mtd1, RNA (guanine-9-) methyltransferase domain containing 1 ( MGI:1196261)
Sequence:	sense strand is shown >T6806 GCGGTGTGCACGCGGATCTGCTCCGGAGTCCTGCAGCCCAGCCGCCGGCCGCAGTCTGGGTACCTGCGGG GCTTCTCTCTGCCGGTGCTGGGGTGCTGGGTGGACCCGCTGGCTTTCCAGGATGCGGGGCGTGGAACGTG GCCTCTGAGAACCCCAGGCGAGAGGAAGCCGTGCTGTAGGAGTCGTTAGGCTCGGCGCTACCTGAAGCGG AAGCCGCGTTCACCCAGATCGTCTCCTGTGTCGCGCTTTAAAGTGGTTCCTCACATTTCTTCAAAATGAA TGTGACTGTCCGTTTCTTAAGACCTTTTGCCAGATGTCTGGTGCCATATACCTTTCATAGGAAGAGAAGT CATTTATATTCAGGAGTTCTGCAGAGATACATGTCCTCCAAAGCACCTTCTTTGTCTTGTCATAATAAGG ACAGTGCATCCCCTCCTGAGCAGCTAGAATTGGATGGGTGGAAAGCTACAATGAAGTCGAGCATTCAAGA AGATGGTGTTTCAGAGGTCTCAGACAAAGATGAAGATTCCCTCGCCTCAACCAGAGAACTAATTGAGATG TGGAGATTGCTTGGCAAAGAAGTACCAGAACATATTACTGAAGAAGATCTCAAAACCCTTATGGAATGTG CCTCTAAATCAGCCAAGAAGAAATACTTACGGTATCTGTATGGGAAGGAAAAGGCGAAAAAAGCTAAGCA GGTAAAGAAGGAGATGAAAGCGGAGGCCAGGGAAGAAGCAAAAAGGGCCAGGTTGCTAGAGACCACCGCA GAAGAACAACAGCAAGACTTCATGTTTCTTCGACTATGGGATCGACAGATCAACATTGCACTGGGTTGGA AGGGTGTCCAGGCTATGCAGTTTGGACAGCCTTTGGTTTTTGACATGGCCTATGATAATTACATGAAACC AAGTGAACTGCAGAATACTGTTTCTCAACTTTTAGAAAGTGAAGGATGGAACAGAAGAAATGTTGATCCT TTCCACATTTATTTCTGCAATCTTAAAATAGATAGTGCTTATCATAGAGAATTAGTTAAACGTTATAGAG AAAAATGGGACAAATTGCTCTTAACAGCAACAGAAAAGTCTCCTGTTGATTTATTTCCAAAGGACAGTAT TATATATTTAACTGCAGATTCTCCCAATGTTATGACTACCTTCAAGCATGATAAAATTTATATAATTGGA TCATTTGTTGATAAAAATACACAGACAGGAACATCCTTAGCCAAGGCCAAACGGTTAAACATAGCAACAG AGTGCCTTCCACTAGATAAATATTTACAATGGGAAATTGGTAACAAAAATCTCACCTTAGATCAGATGAT CCGAATTTTGCTCTGCCTGAAAAACACTGGTAGCTGGGAAGAGGCTCTCAAGTTTGTTCCCAGGAGAAAG CATACCGGTTATTTAGAGGTTTCTGAGCAATCTCAGGAACTTGTTAGGAAACTGAAGAAGACAAAGACTT TAAATTCATTTCGAAAAGGCTCCTTAAATGTGCGCACGTGGAAAAGGTGACCTAAGTGAGAAAGTATGGT GAAAGAAGAAAACAGGCTGAAAGTATGGTGACAAGAATGTAGTTATTCCTAAATGGAATTATCTCTTAAA GTCAGTCTCTCTAGACTAACAGTAGAGGTTTGCCTATTTTTTCTTCTTCTTTGCTTTTTTGCAACAGGAT TTCCATGTAGCCTTGTCCTCCATCAAGGCCAGACTAGACGTATGTATCAGCAAAACTTTGAAAGTATGTT TTATTTGTTGTAAAAACAATCAAGTTTATAATTAATCTAGAATTAAACACAATAAAAGTTAATATTATTT ATTTTTAATCGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710019 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710019 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11248 same embryo
	EMAGE:11246 same embryo
	EurExpress:euxassay_006517 same experiment
	MGI:4827708 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source