EMAGE mouse gene expression spatial database

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EMAGE:11250

Pigq phosphatidylinositol glycan anchor biosynthesis, class Q ( MGI:1333114)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006606. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006606_01	euxassay_006606_02	euxassay_006606_03	euxassay_006606_04

euxassay_006606_05	euxassay_006606_06	euxassay_006606_07	euxassay_006606_08	euxassay_006606_09

euxassay_006606_10	euxassay_006606_11	euxassay_006606_12	euxassay_006606_13	euxassay_006606_14

euxassay_006606_15	euxassay_006606_16	euxassay_006606_17	euxassay_006606_18	euxassay_006606_19

euxassay_006606_20	euxassay_006606_21	euxassay_006606_22	euxassay_006606_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11250_wholemount_strong.wlz

11250_wholemount_moderate.wlz

11250_wholemount_weak.wlz

11250_wholemount_possible.wlz

11250_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11250_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7377
Entity Detected:	Pigq, phosphatidylinositol glycan anchor biosynthesis, class Q ( MGI:1333114)
Sequence:	sense strand is shown >T7377 CCACGCGTCCGAGCCGGCGGAAATGGTGCTGATGTCCCGCGGCGGGCCTGGGAGCCGCAAGCACGGTCGT CCTCCCGGGCCCGCCGAACGCAGTGCCCAACCCAGTTCCTTCCCTACCGCTCGGGGACGCGGCCCCGGGG ACCAGCTGGGCCCCCAGCATGGTGCTCAAGGTCTTCTTCCCCACGTGCTGTGCCTCGGCAGACAGTGGCC TGTTAGTGGGACGATGGGTTCCAGGACAGAGCAGTGCTGTGATCCTGGCCGTGGTGCACTTTCCCTTCAT CCCCATCCAAGTCAAGGAGCTCCTGGCCCAGGTGCAGAAGGCCAGTCAAGTACCAGTGGCTGTGCTAGGG ACCTGGTGCCACCGTCAGCAGGAACCGCAGGAGAGCCTGGGGAATTTCCTGGAGGGCCTGGGCACCATCT TCTCCCATGACCCCTGGTTGCAGCTCTGCCGGGAGAGGGGTACCAGACTCTGGAGCTGCAAGGCCACTTA CCCGCAGATGTCTAACCCTCTGGACATGCACCCCGAAGAGCAGGTCATGCTCATCTTCTATGATCAGCGC AAGCTGCTGCTCTCCTGGTTGCATCCGCCTCCAGTCCTGCCTGCCTGCCAGATGGGAGACACCACAGCCA GCACCGGAGGCCTAGCTGACATCTTTGACACAGTGGCACGCAGCGAGGTGCTCTTCCGAAATGACCAATT TGATGAGAGACCCGTACGCCTGAGCCACTGGCAATCAGAGGGAGTGGAGGCCAGCATACTTGTGGAGTTG GCAAAGCGGGCCTCTGGGCCTGTCTGCCTGCTGCTGGCTTCTCTGTTGTCCCTGATCTCAGCAGCTAGTG CTTGCCGGCTATGGAAGCTGTGGCCACTTTCCTTCATCAGAAGCAAGCTCTCCACTTGTGAGCAGCTCCA TCACCGGCTGAAGCACCTCTCCTTCATCTTCAGCACGGAGAAAGCCCAGAATCCCATGCAGCTGATGAGG AAGGCCAACATGCTGGTTTCTGTGCTACTGGATGTGGCCCTTGGCTTGCTGCTGCTCTCTTGGCTCCACA GCAATAACCGAATTGGACAGCTGGCCAATGCCCTGGTCCCTGTGGCTGATCGCGTGGCTGAGGAGCTCCA GCATCTGCTGCAGTGGCTGATGGGTGCTCCTGCTGGGCTCAAGATGAATCGGGCACTGGATCAGGTGCTA GGCCGCTTTTTCCTGTACCACATCCATCTGTGGATAAGCTATATCCACCTTATGTCCCCCTTTATTGAGC ATATCCTGTGGCATGTGGGACTCTCAGCCTGCCTTGGACTGACTGTTGCTCTGTCCATCTTTTCGGATAT CATCGCCCTTCTCACCTTCCACATCTATTGCTTCTATGTCTACGGTGCCAGGCTGTACTGCCTGAAGATC TATGGCCTTTCCTCTCTCTGGCGTCTGTTCCGGGGGAAGAAGTGGAATGTTCTGCGCCAGCGAGTGGATT CTTGTTCCTATGACCTTGACCAGCTCTTCATTGGGACCTTGCTCTTCACCATCCTGGTCTTCTTGCTACC CACCACCGCTCTGTACTACCTGGTATTCACCCTGCTCCGGCTCCTGGTGATCACTGTGCAAGGCTTAATC CATCTACTTGTAGACCTCATCAATTCCCTGCCACTATACTCCCTTGGCCTTCGACTCTGCCGACCCTACA GGCTGGCAGCTGGTGTGAAGTTCAGAGTTCTGGAAAAGGAGGCAGGCAGACCCCTCCGCCTCCTGATGCA GATAAACCCCCTGTCCTATAACCATGTGATGCACATCTACCGTCTGCCTCGCTGTGGCTGCCATCCCAAG CACTCCTGGGGTACCCTGTGCCGAAAGCTGGTCTTCGGAGAGCTCATCTACCCCTGGAGGCAGAGAGAGG ACAAACAGGACTGAGGAGCTGGACACAGCCTACCCATAGCTACTGCACAACCATCACGCCTCCTCTCTGC CAGGACAGCACTGACCACGGGGTCATCTCAGGTCCCACAAGGGCCAACCAAACACTCTGTGTGCTGAATG TAGGCAGGCCTTACCCTTGAGTTACTACTTTGCTCTCGGACCCTGCCAGCTTCAGTCCTGCACTCTATCT AGCACAGTGGTTCTCAACCGTCCCAAAGCTGACCCTTTAATACAGTTCCTCATGTTGTGGTGACCCCAAC CATAAAATTATATTCATTACTACTTCATTACTGTTAATTTTGCTACTGCTATGAATCATGATATAATATA AATATCTGTGTTTTCTGACAGTCATAGGTGAACCCTGTGAAAGTGTCATATGACACCCACCCCAAAGGGT TGAGAACCACTGCTCTAGTATCACCTATGTAGGGACCCACATGGTTTTTGTAGTCATTACAGTGAGCCTG ATTCCTAGGAGTTCCCGGACCCCGTGGGATATGCTGGCAGGTGCTCTTGGCTGCTTTATTGGGTGGGAGC ACTCCTGGGCACCTGGTGTACTTCATAAGCCTCCGCAGAACATTTCCATTGGGAAACAGGCAGCTGCTGA TCCCTTAGCCCCTCGAACTAATCCCTCACCCTGTACTTCATTGGGCTTACTTGCTACTCTGGAGATTGCT TTGTGAAGTTGCTGTTAATAGGCCTTTGGGTACCCTTTGCTTGAAGTTATATCAACTGAGGTGATTGGAT ACCTGCCAACCTGGCTAGTGCTCCATGTATGAATAGGCCCTTTGGCAAAATATTCTATGTTCTCTTATAG ACTCCTTATGGGACCAGATAGCTTCCTTCTGGACAGGCACTTCATGGCTCATTGTGTGAATGATCTTGGG GTGGTGAAATGGCCAGGGTTGGTTCCCTAACACTTAGTGGGACCCCATGTGAGCTAGTCCCTAGGGGGCA GGAAACCTCACTGCCTGAATTGTCTACTGTGGGCAGGAGGGGACAGCCGGCCCAGCCTGTCTTTCAGGGG CACTTGGGACTATTGACATTGGTCTGTCTGTGCCTGCTTCCTCTTGCTATAGGTCACAGCCACTCTTAAG CAGCCAGTGGAGGGTTTTGGTGAAGATGAATCCTGATCCTGTGATGACAATAAAAATCTCATGAATCTGG AGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193343 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193343 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11249 same embryo
	EMAGE:11252 same embryo
	EMAGE:11251 same embryo
	EurExpress:euxassay_006606 same experiment
	MGI:4827203 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source