EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11258

Cap1 CAP, adenylate cyclase-associated protein 1 (yeast) ( MGI:88262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006492. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006492_01	euxassay_006492_02	euxassay_006492_03	euxassay_006492_04

euxassay_006492_05	euxassay_006492_06	euxassay_006492_07	euxassay_006492_08	euxassay_006492_09

euxassay_006492_10	euxassay_006492_11	euxassay_006492_12	euxassay_006492_13	euxassay_006492_14

euxassay_006492_15	euxassay_006492_16	euxassay_006492_17	euxassay_006492_18	euxassay_006492_19

euxassay_006492_20	euxassay_006492_21	euxassay_006492_22	euxassay_006492_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11258_wholemount_strong.wlz

11258_wholemount_moderate.wlz

11258_wholemount_weak.wlz

11258_wholemount_possible.wlz

11258_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11258_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6655
Entity Detected:	Cap1, CAP, adenylate cyclase-associated protein 1 (yeast) ( MGI:88262)
Sequence:	sense strand is shown >T6655 CCCACGCGTCCGGGAACTCTTAAGCAGGTGGAACATTATGGCTGACATGCAAAATCTTGTAGAAAGATTG GAGAGGGCAGTGGGCCGCCTGGAGGCAGTGTCACATACTTCAGACATGCACTGTGGATATGGAGACAGCC CTTCAAAAGGAGCAGTTCCATATGTGCAAGCATTTGACTCGCTGCTTGCCAATCCCGTGGCAGAGTACTT GAAGATGAGTAAGGAGATCGGGGGAGATGTGCAGAAACACGCGGAGATGGTCCACACAGGCCTGAAGTTG GAGCGAGCTCTCCTGGCTACAGCTTCTCAGTGCCAGCAGCCAGCTGGTAATAAACTTTCTGATTTGTTGG CACCTATCTCGGAGCAGATCCAAGAAGTTATAACCTTCCGGGAGAAGAACCGAGGCAGCAAGTTTTTTAA TCATCTATCTGCTGTCAGTGAAAGCATCCAGGCCTTGGGCTGGGTGGCTCTGGCTGCGAAACCTGGCCCC TTTGTGAAAGAGATGAATGACGCGGCCATGTTTTACACAAATCGTGTCCTCAAGGAGTACAGAGATGTGG ATAAGAAGCATGTGGACTGGGTCAGAGCTTACTTGAGTATATGGACGGAGCTGCAGGCTTACATCAAGGA GTTTCATACTACTGGCCTGGCCTGGAGCAAGACGGGGCCTGTGGCAAAAGAACTGAGTGGATTGCCATCT GGACCCTCTGTGGGATCAGGCCCACCTCCTCCCCCACCGGGCCCGCCTCCTCCCCCAATTCCTACCAGTT CTGGTTCTGACGACTCTGCATCACGCTCAGCACTGTTTGCACAGATTAATCAGGGGGAAAGCATCACACA TGCCCTGAAACATGTATCTGATGACATGAAGACTCACAAGAACCCTGCCCTGAAAGCTCAGAGCGGTCCA GTTCGGAGTGGCCCCAAACCATTCTCTGCACCTAAACCCCAAACTAGCCCCTCCCCCAAACCAGCCACAA AGAAGGAACCAGCTCTGCTGGAACTGGAAGGCAAGAAATGGAGAGTGGAAAACCAGGAGAATGTTTCTAA CCTGGTGATTGATGACACTGAGCTGAAGCAGGTGGCTTACATCTACAAGTGTGTCAACACAACATTGCAA ATCAAGGGCAAAATTAACTCCATTACAGTAGATAACTGTAAGAAGCTTGGCCTGGTGTTTGATGACGTGG TGGGCATTGTGGAGATAATCAATAGTAGGGATGTCAAAGTTCAGGTGATGGGAAAAGTGCCAACCATTTC CATTAACAAAACAGATGGCTGCCATGCTTACCTGAGCAAGAACTCCCTGGACTGTGAGATAGTCAGTGCC AAATCTTCTGAGATGAATGTCCTCATTCCTACCGAAGGCGGTGATTTTAACGAGTTCCCAGTCCCCGAGC AGTTCAAGACCCTGTGGAACGGACAGAAGTTGGTCACCACAGTGACAGAAATCGCTGGATAAGCAAATAC ATGGGTCCTGTGCCCTCTCCCTTCACACCATGGGATACATCTGTATGGAGACGGTTCTTTTCTAGATTTC CTCTACCTTTCTGCTCTTAAACTGCTTCTCTGCTCTGAGAAGCCCAGCTACCTGCCTTCACTGAAATATA CCTCAGGCTGAGACGGGCGGGACAGCAGGTCAGTGGATCTTCACCGGAAGGTGCAGCTTCTCGGCATCAG CGCAGCTGCACCACAGGCTGAGCTTACAGAGCTTCAGGCCCAGACTGGGGCACCGAAGCTTTCAGCTTTG GGGGTCATTCTACCAACAATCAGTCCCTGAGGGTTCACAACACCCTGGAACCAAACATCAGAATGCACAC TATTCTCCTAACAGTGAGCATGGGGGGCAGGAGCCAGGGAGTCTGTTAGGTTCTAACCAACCTAGTTTTC ATTGTTGACAAGTGAGGCCAGGCTTCCACTCGACCAAAAGCTAAGGATTGCCACCCAGCATTCCAAGCAG ACTTGTTTCCTAAAAGCATCCTTCATCTGCATTCCATCCTGGGTCAGCATCAACCACAACATAGCAGGCG TTCCCTTGCAGCTGCAGTACAGTAGCAGTGTCCTTTTCTGAGGAAGGAAGTCTTAAACCCTTTCCCCCCA CCCCCTGGGATGACTGCCTTGATTCCCATGGGGAACAACTTTAAAGAAAACCACCACAGCATTCCAAGGA GTCTGGCGTTCCTCCTCCCTGCCAGATGACTGTCATTGCCTCCACTCACCCTTCTGCTTGTCACTCGGCA GCTTAGGGCCTCTGCGCAGGAACCTGACTAGGACTATGACCTCCACGGCGGCTGTTTAGGTCTCGACTTA CATCAGAATCTTGCACAGCATTGCAAAATCCCAGTGCTCCCTCTAGGACACACTGACCAAAATATGCAAC CTTTCCTTTCCTTTTTTTTTTTTTTTGGTGGGAAAAGAGGAAGAAAGATTGTCCTGTGACTTCTGCCCAT TACCTGAGGCCTGTGGAAATAAACCTTTATGTACTTAAAATTATACAGAAAATAGAATAAAGTTAATACC AAACTTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600514 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600514 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11259 same embryo
	EMAGE:11260 same embryo
	EMAGE:11262 same embryo
	EMAGE:11261 same embryo
	EurExpress:euxassay_006492 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source