EMAGE mouse gene expression spatial database

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EMAGE:11266

Prcp prolylcarboxypeptidase (angiotensinase C) ( MGI:1919711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006670. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006670_01	euxassay_006670_02	euxassay_006670_03	euxassay_006670_04

euxassay_006670_05	euxassay_006670_06	euxassay_006670_07	euxassay_006670_08	euxassay_006670_09

euxassay_006670_10	euxassay_006670_11	euxassay_006670_12	euxassay_006670_13	euxassay_006670_14

euxassay_006670_15	euxassay_006670_16	euxassay_006670_17	euxassay_006670_18	euxassay_006670_19

euxassay_006670_20	euxassay_006670_21	euxassay_006670_22	euxassay_006670_23	euxassay_006670_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11266_wholemount_strong.wlz

11266_wholemount_moderate.wlz

11266_wholemount_weak.wlz

11266_wholemount_possible.wlz

11266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07 weak expression: see section 01 02 21 22 23 24

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

axial musculature

weak

regional

weak expression: see section 03 04 05 06 19 20 21 22 23 24

thymus primordium

weak

regional

weak expression: see section 12 13 14 15 16

diencephalon meninges

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon meninges

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

tongue muscle

weak

regional

weak expression: see section 13 14 15 17 18 19

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 19 20 21 22 23 24 moderate expression: see section 12 13 14 weak expression: see section 18

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24 moderate expression: see section 12 13 14 weak expression: see section 15 18

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

basioccipital bone

moderate

regional

moderate expression: see section 05 06 22 weak expression: see section 23

orbito-sphenoid

strong

regional

strong expression: see section 02 03 moderate expression: see section 04 05 06 07 weak expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7113
Entity Detected:	Prcp, prolylcarboxypeptidase (angiotensinase C) ( MGI:1919711)
Sequence:	sense strand is shown >T7113 GTGGCTGCCATCCCCAGCCATGGGCTGCCGGGCTCTCCTGCTCCTGTCTTTCCTATTACTCGGGGCCGCC ACAACCATCCCGCCTAGGCTAAAGACCCTCGGCAGCCCGCATTTGTCAGCCAGTCCCACACCCGACCCTG CTGTAGCCCGTAAATACTCAGTTCTTTACTTCGAACAGAAGGTTGACCACTTTGGGTTTGCTGACATGAG AACTTTTAAACAGCGGTACCTAGTCGCTGATAAACACTGGCAGAGAAATGGTGGATCAATACTTTTCTAC ACTGGTAACGAAGGGGATATTGTCTGGTTTTGCAATAATACGGGGTTCATGTGGGATGTGGCTGAGGAGC TGAAAGCTATGCTGGTGTTCGCTGAGCATCGATACTATGGGGAGTCCCTTCCCTTTGGTCAGGACTCCTT CAAGGATTCCCAACACTTGAATTTCCTGACATCAGAACAGGCTCTGGCTGATTTTGCAGAGTTAATCAGA CACTTGGAAAAAACAATCCCAGGAGCTCAAGGTCAACCTGTCATCGCCATCGGGGGCTCTTATGGTGGCA TGCTTGCAGCCTGGTTCAGGATGAAATATCCTCACATTGTAGTTGGAGCTCTTGCAGCTTCTGCCCCTAT CTGGCAGCTTGATGGTATGGTTCCCTGTGGTGAGTTTATGAAGATTGTAACTAACGATTTCAGGAAAAGT GGTCCTTATTGTTCAGAAAGTATCCGCAAATCCTGGAATGTCATTGACAAACTCTCAGGTTCAGGCAGTG GTCTCCAGTCGCTCACGAACATTCTTCACCTATGTAGTCCCTTGACTTCTGAGAAAATCCCAACTTTAAA AGGATGGATAGCTGAGACCTGGGTGAACCTGGCCATGGTGAACTACCCTTACGCATGCAACTTTTTGCAG CCTTTGCCTGCTTGGCCCATCAAGGAGGTGTGCCAATATTTGAAAAATCCTAATGTATCGGATACAGTGT TGCTGCAGAATATTTTCCAAGCTCTGAGTGTATATTATAACTATTCAGGCCAAGCAGCATGCCTGAATAT TTCACAAACGACTACGAGCAGTCTGGGCTCCATGGGCTGGAGCTTTCAGGCCTGCACAGAAATGGTCATG CCCTTTTGTACTAATGGTATTGATGACATGTTTGAACCCTTTCTGTGGGACTTGGAGAAGTATTCCAATG ACTGTTTTAACCAGTGGGGAGTGAAACCACGGCCTCATTGGATGACTACCATGTACGGTGGCAAAAACAT CAGTTCACACTCAAACATCATTTTCAGCAATGGTAAGTTAGACCCGTGGTCAGGAGGCGGAGTAACGAGA GACATCACAGACACCCTGGTGGCTATCAACATCCCTGACGGGGCCCATCATTTAGATCTCCGAGCCCACA ATGCCTTCGATCCTTCCTCTGTGCTGTTATCTCGATTACTGGAAGTTAAACACATGAAGAAGTGGATTTT AGATTTCTATAGCAATATCCAATGAACAATTTTTTAAAATTAATTTTTTTTGTTTTCTTTTATGTTCCCT CCACCCACATACCCATTCACTTTTATGTAATTCACTTATATGTAATTACTTTCTCTCATTTGTCATTATA TTTGTTGGGCCGAGGCCAAGACACAAGAGAGTGACAGGGGTGGGCACAGACATCCCACCCAGGTGACTGC TCCGGCCTCACTGTCTTTGTGTCATCGTGGCAGCTCACAGGCCATCAGAACTGGCATGGCTCCTCACTGC CTTTCACCAGAGGGAGAGCCCCTCTCTCAGCAGCAGGGAGGTCTCCTTGGTTCTGAGGCTGAAGTCTCTC TGGCCCATTGTAAGTCCCCACTCCTGCCACAAAAGGAAAGCAGAAGCTGGGTTCAAAACGCTGCAGCTGC AGCTGATAGAATGTGGAATGCCAGTCCAGGAAACCGGGATGCTTCCTGTGAGCTCACTCAGTGAGTGATT CTGAACTGGGCTGCCATAAAGAAGAAAGCAGAGACCTACCTGTGTGTTGCTTCTGTCTCCTTCCAGCTGT TGGGAGGTCAGCGTGCCTGGGCTGGCTGCTTTTGGAAAGTCCTTTCTTTATCTCTTTCCCACTTGGCAGC TCTCCTTAGTTCTGGGACATTTGCCACTGTGGTACTGCCAGGAAGGAATCTGGCCTTGTCCTGCTCTCTT TGTTTCCAAGGTCAATTTTATGGTGGTCTGGCTACATTCTGCCAATTTAGGAAAAGTGCTGTTTTTGTTT TTTTTGTTTTGTTTTGTTTTTTTACTTTAAAACTGAACAAATTCTGTTAATAAAAATTCTTTAAAAATTG CAAACCATTTATTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4017462 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017462 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11264 same embryo
	EMAGE:11265 same embryo
	EurExpress:euxassay_006670 same experiment
	MGI:4827396 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source