EMAGE mouse gene expression spatial database

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EMAGE:11273

Atf3 activating transcription factor 3 ( MGI:109384)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006681. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006681_01	euxassay_006681_02	euxassay_006681_03	euxassay_006681_04

euxassay_006681_05	euxassay_006681_06	euxassay_006681_07	euxassay_006681_08	euxassay_006681_09

euxassay_006681_10	euxassay_006681_11	euxassay_006681_12	euxassay_006681_13	euxassay_006681_14

euxassay_006681_15	euxassay_006681_16	euxassay_006681_17	euxassay_006681_18	euxassay_006681_19

euxassay_006681_20	euxassay_006681_21	euxassay_006681_22	euxassay_006681_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11273_wholemount_strong.wlz

11273_wholemount_moderate.wlz

11273_wholemount_weak.wlz

11273_wholemount_possible.wlz

11273_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11273_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

strong

regional

strong expression: see section 15 20 21 22

femur

strong

regional

strong expression: see section 10 19

heart ventricle

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7063
Entity Detected:	Atf3, activating transcription factor 3 ( MGI:109384)
Sequence:	sense strand is shown >T7063 CCACGCGTCCGCTGGGATTGGTAACCTGGAGTTAAGCGGGCTCCCTGCCAACGCGAGGGCTTTAAAAGGG GTGATGCAACGCGCTCCCAGCCACAGTCTCACTCAGCGAGACGCCGCGCACAGTGCTTCCCCAGTGGAGC CAATCGGCTAACCCGCGCTCCGGCAGAGTCCTTGGCGCTCGCCCGCCGGCGGGACAGACCACCCGCCTCT GGCCGTTCTCTGGACCCTGGCCGCCCCGAGCGAAGACTGGAGCAAAATGATGCTTCAACATCCAGGCCAG GTCTCTGCCTCAGAAGTCAGTGCGACCGCCATTGTCCCCTGCCTCTCACCTCCTGGGTCACTGGTATTTG AGGATTTTGCTAACCTGACACCCTTTGTCAAGGAAGAGCTGAGATTCGCCATCCAGAATAAACACCTCTG CCATCGGATGTCCTCTGCGCTGGAGTCAGTTACCGTCAACAACAGACCCCTGGAGATGTCAGTCACCAAG TCTGAGGCGGCCCCTGAAGAAGATGAGAGGAAAAGGAGGCGGCGAGAAAGAAATAAAATTGCTGCTGCCA AGTGTCGAAACAAGAAAAAGGAGAAGACAGAGTGCCTGCAGAAAGAGTCAGAGAAACTGGAGAGTGTGAA TGCTGAGCTGAAGGCCCAGATCGAGGAGCTGAAGAATGAGAAACAGCATTTGATATACATGCTCAACCTG CACCGGCCCACCTGTATCGTCCGGGCTCAGAATGGACGGACACCGGAAGACGAGAGGAACCTCTTTATCC AACAGATAAAAGAAGGAACATTGCAGAGCTAAGCAGAGGTGGCACGGAGGCAATTGGGGAGTTCTTACTG AATCCTCCTTTTCCACCCCAAACCCTGAAGCCATTGGAAAACTGGCTTCCTGTGCACTTCTAGAATCCCA GCAGCCAAGAGCCGTTGGGGCAGGAGGGCCTGTGGTGACCTACTGCATTGTCCCACTCTGACCCCGAGTG AACCGTGGAGCAGGCAGGAGCATCCTTTGTCTCACCAATTCCAGGATTTAGGCCTTATCATCCCGGCCAG TCTCAGATGACCTAGCTGGCCTCAAGCTGGGGTCCTATGCAAAGCAGGATCCCACTAATGGATTCAGGCA GAAGTGTCTACCTTGATAGGTGGGGTGGGACCACATCCTCCACTGTGGCTGACAACGCCCTTCCAAGGGA ATATGGAATGAGAACATTCATTATTGAGGTTGTCCAATGGCCAGGGTATGCTTTCTAGAAAATATGCTGT TCTGTCCCAGAATGACTGTGCATAGAGTATCCTTTTCAGAGCCTGGTGTTGTGCTATTTAGATGTTTGTC TTGCACAACATTGGCATGATTTTTCCGGGAGTTTCATCAGATCTGATTTCTGAGAGTCTGGGGATCTGCC ATGGTGGAAAGTGCCCCTCAAAAGCATTTGTGTGGCCACATGAACTGGCTGGCACCAGGGGAGTGAAACT GGCTGATGACCAGCTGAGCCACTTTGTGCCAACAGAGGATGGACGACACCTTTCCCTGTACCCACTGCAG AGGAAGAACCCTGGGCACAGCAGCTTTGTCCTTGGCTACAAACTGTTACAACGTCACACAATGAAGGCAC AAAGTCCAACTTTCAAAGGGTGTAGGACTCCATACTCAGTGACAGGGCAGGAAGAGCCAAAGATAACCAC AGCCACAGCCTGTGGAGACCAGGGTTGGAAGCCAGGTGCAGGGCCAGGCATCTGCATTGTGGGATGTTAA TGGCACTTTTGTCTTGTAGCTATTTTGAGATGTGGTCCAGAGCATTTCAGCTGGGAGATCTCCCTCTGGC CACCAGGACTCTGGCTACTGTTAAAATCCTGATGTTTCTGTGGAATCCTCAGTGTTTAATCCTACTCAAT AGTATCATTACAGTTTTCTGTAAGAGAAAATATTACTTATTTATCCCAGTATTCCTAGCCTGTCAACATA ATAAATATCGGAACAAAACCTGGTAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4011514 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4011514 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11274 same embryo
	EMAGE:11272 same embryo
	EMAGE:11271 same embryo
	EurExpress:euxassay_006681 same experiment
	MGI:4823291 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source