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EMAGE:1133

Cbs cystathionine beta-synthase ( MGI:88285)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1133
Cbs 10.5

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1133Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1133_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1133_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2663877
Entity Detected:Cbs, cystathionine beta-synthase ( MGI:88285)
Sequence:sense strand is shown

>MGI:2663877
CCGTGTTGCCCACGGTCACCTGTGAGGACACCATCGCCATCCTCCGGGAGAAGGGTTTTGACCAGGCACC
TGTGGTCAACGAGTCTGGGGCCATCCTAGGGATGGTGACCCTCGGGAACATGCTGTCATCCCTGC
Notes:Full length cDNA clone for this gene:BC013472
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Valeria Marigo, TIGEM, Via Pietro Castellino 111, Naples, Italy 80131
Submitted by:Cristina Caccioppoli, TIGEM, Via Pietro Castellino 111, Naples, Italy 80131
Experiment type:screen
References:[ doi:10.1038/nature01178] [ PMID:12466854] Reymond A, Marigo V, Yaylaoglu MB, Leoni A, Ucla C, Scamuffa N, Caccioppoli C, Dermitzakis ET, Lyle R, Banfi S, Eichele G, Antonarakis SE, Ballabio A 2002 Human chromosome 21 gene expression atlas in the mouse. Nature (420):582-6
Acknowledgments: This data was produced as part of this consortium  Human Chromosome 21 Expression 'Atlas'  Telethon Institute of Genetics and Medicine Naples ; Max Planck Institute Hannover; Division of Medical Genetics Geneva, Naples, Hannover, Geneva, Italy, Germany, Switzerland
Links:MGI:2666361 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE