EMAGE mouse gene expression spatial database

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EMAGE:11357

Tceal1 transcription elongation factor A (SII)-like 1 ( MGI:2385317)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006652. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006652_01	euxassay_006652_02	euxassay_006652_03	euxassay_006652_04

euxassay_006652_05	euxassay_006652_06	euxassay_006652_07	euxassay_006652_08	euxassay_006652_09

euxassay_006652_10	euxassay_006652_11	euxassay_006652_12	euxassay_006652_13	euxassay_006652_14

euxassay_006652_15	euxassay_006652_16	euxassay_006652_17	euxassay_006652_18	euxassay_006652_19

euxassay_006652_20	euxassay_006652_21	euxassay_006652_22	euxassay_006652_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11357_wholemount_strong.wlz

11357_wholemount_moderate.wlz

11357_wholemount_weak.wlz

11357_wholemount_possible.wlz

11357_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11357_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

tongue muscle

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7055
Entity Detected:	Tceal1, transcription elongation factor A (SII)-like 1 ( MGI:2385317)
Sequence:	sense strand is shown >T7055 CCTTGATCGAGAAGGAAAGCAGAAAATAATTGCTTGAAGAAACTTCTCAACATGGAAAACACGCGCAGTG AAAATGAAGAGCAACCAGAGAGCACTCTGAAGATCGATGAAGAGCAGCCTGCAGTGGAGCAGTCTCCTGA AAATCAGTGCTCTGAGGAGGATCAATCCTCGGAGGATCTGTCCTCCGAGGAGCAGTCCTCCGAGGAGGAG TTCTTTCCTGAAGAGCTCCTGCCCGAGCTGCTCCCAGAGATGCTGCTGTCTGAAGATCGTCCTCCGCAGG AGTGCCTCTCACAGAAGAACCAGTTCGAAGACCGTATTCCCATGGAGCAACCTCCCTGCGGGGTGGGCAA ACACAAGCTAGAGGAGGGAAGCTTCAAAGAAAGACTGGCCCGTATCCGCCCTCAATTCATAGGAGACATT CATGGCAGAAATTTAAGTAATGAAGAAATGATACAGGCAGCGGATGAGTTGGAAGAGATGAAGAGAGTAC GAAACAAACTGATGATAATGCATTGGAAGGCAAAACGGAGCCGCCCTTACCCTATTTAATATGCTCCACT TTTCATTATTTTGCTTGTTAATAGCATTTGCTACCTGGCCCTTGTGTTTGCATCTTCTTATATGCCCTTT CCCATCTTAGTTGTAACAGTTTTATTCAGGTTGTGCAGTTTTATTTCAAGTGTTTGACATGTAACTGGCA CGGGTCTTTGTTTTTGTTTTTGTTTTCAGACCTTCTAAAATCTGAGTGTGATCCATTTGCATGGAAAGAT ATACATTATATATGTGAGGTGATAAAAGCAAAAGGTATATGCATAGCATCCCATTTTTGTAAAAGGAAAA CACATTTATATATTAATATGCAAAGAAAAGCTAAAAACTATATACTTAAAGACTAACATTGGTTATCTGT GTGGTAAAGATAATAAGTGATTTTTATATTTTTTTAAATTTTGCTTTATCTAAAGTTTCATTTTTCATGA TGAATACATTTTATTTATGTTGCACAACAAACAATATAATGAAAAATGTGAAGCAAGCCTAAAATCAAAA AGTGTTAGTCAGCTTATAAAGAAAATGTGCTCCTTAATAAATATTTATCAGAACTTAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:4010035 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4010035 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11358 same embryo
	EMAGE:11355 same embryo
	EMAGE:11356 same embryo
	EurExpress:euxassay_006652 same experiment
	MGI:4828629 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source