EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11359

Smoc2 SPARC related modular calcium binding 2 ( MGI:1929881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006650. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006650_01	euxassay_006650_02	euxassay_006650_03	euxassay_006650_04

euxassay_006650_05	euxassay_006650_06	euxassay_006650_07	euxassay_006650_08	euxassay_006650_09

euxassay_006650_10	euxassay_006650_11	euxassay_006650_12	euxassay_006650_13	euxassay_006650_14

euxassay_006650_15	euxassay_006650_16	euxassay_006650_17	euxassay_006650_18	euxassay_006650_19

euxassay_006650_20	euxassay_006650_21	euxassay_006650_22	euxassay_006650_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11359_wholemount_strong.wlz

11359_wholemount_moderate.wlz

11359_wholemount_weak.wlz

11359_wholemount_possible.wlz

11359_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11359_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7354
Entity Detected:	Smoc2, SPARC related modular calcium binding 2 ( MGI:1929881)
Sequence:	sense strand is shown >T7354 AGAGCGCACGGAGGGGCCGTGCGGTCTCCACCAGCGGCCATAGGACCGCGGAGCAGTTCTAGCCCGCTCG CCCGCACGTTCGCACACCGGATCTTCGCCGAGTGCCAGGGCGCAGCGCGTGGGGCGTCTGCCTCGCTTGG TCCCCTCCAGCGTCACCATGCTGCCGCCACAGCTGTGCTGGCTGCCGCTGCTCGCTGCCTTGCTGCCGCC GGTGCCCGCGCAGAAGTTCTCAGCGCTCACGTTCTTGAGAGTCGATCAAGACAAAGACAGAGACTGCAGC CTGGACTGCCCAAGCTCCCCTCAGAAGCCACTCTGTGCCTCGGACGGGAGGACCTTCCTGTCCCGATGTG AGTTCCAGCGGGCCAAGTGCAAAGATCCACAGCTGGAGATCGCTCACCGTGGGAATTGCAAAGATGTGTC CAGGTGTGTGGCTGAGAGGAAGTATACCCAGGAGCAGGCCCGGAAGGAGTTCCAGCAAGTGTTCATTCCA GAATGCAATGATGACGGCACCTACAGTCAGGTCCAGTGTCACAGCTACACAGGATACTGTTGGTGTGTTA CACCAAATGGAAGACCCATCAGTGGCACTGCTGTGGCCCACAAGACACCCAGGTGCCCCGGTTCAATAAA TGAAAAGGTGCCGCAGCGGGAAGGAGCAGGGAAAGCAGATGATGCTGCAGCCCCAGCATTGGAGACTCAG CCCCAAGGAGATGAAGAAGATATTGCCTCACGCTACCCTACACTCTGGACCGAGCAAGTTAAGAGTCGGC AGAACAAGACCAATAAAAATTCAGCATCCTCCTGTGATCAGGAGCATCAGTCAGCTCTTGAGGAAGCCAA GCAGCCCAAGAATGACAATGTAGTGATCCCCGAGTGTGCACATGGTGGTCTCTACAAGCCAGTGCAATGC CATCCATCCACCGGATACTGCTGGTGTGTGCTAGTGGACACTGGACGGCCCATTCCCGGGACCTCCACAA GGTATGAGCAACCTAAGTGTGACAACACAGCCCGAGCTCACCCAGCGAAGGCCCGGGACCTGTACAAGAA CAGGCCACTGCAGGGTTGTCCTGGTGCCAAAAAGCACGAGTTTCTGACAAGTGTCCTGGATGCGCTCTCC ACAGACATGGTTCATGCCGTCTCTGACCCCTCTTCCTCTTCTGGCAGGCTGTCAGAGCCAGACCCCAGCC ACACCCTGGAGGAGAGGGTTGTACATTGGTACTTCAAGCTGCTTGATAAGAACTCTAGTGGAGACATTGG CAAGAAGGAGATCAAACCCTTTAAGAGGTTCCTGCGAAAGAAATCCAAGCCCAAAAAGTGTGTGAAGAAG TTTGTGGAGTACTGCGACATGAACAATGACAAGTCCATCACCGTGCAGGAGCTCATGGGCTGCTTGGGTG TCACCAGAGAGGAGGGTAAAGCCAACACCAGGAAGCGCCACACCCCCAGAGGAAATGCTGAAAGTTCTTC TTCCTATAGACAGCCCAGGAAACAAGGATGAACGGCTGACTACTCAAGACAGTTCCTAGACATGTGGGAA TTTTCCCTCACCAAAGAGCAATTAAAAACAAAAAACATATAGTATTTGCACTTTGTACTTTAAATGTAAA TTCACTTTGTAGAAATGAGATATTTAAACGGACTGTTGTGATCTGTGAAAACGGAAGGCTGGCTTCGGGA AATTAATCACATACAATGTATGTGTCCTTGTTTGACCTTCAAGATCTGTGCTGGTGGGGTGGGTTTTAAA TGCATTTCAACTTCACTTCCTCGTCCCTCTGTGGAGGGCCTGGTTTATTCCCTTGCCCTTTCCTCTGCCT GCAGTCTCCGTTGGCTTGCTCTCCGTGCTCTTGATGCCAGCCAGAGCCACCGTATCCTTAGAAGCTTGCA GTGGTTGCCTGTTTCATAAAGTTTTGCTCTCTAGAGATGTCACTCACACCATGGGTCACACAGGCACTAG CTCAGGCATAGCCTTAACTTCCAGTAGCCCCTGCTTGAGACTATGCTGTTAGGCTCCAGTTTTAGCTTCT GCTTGAGCCAACCCTACTGTTAAATCCCCGGGGAGCTGAATGGACAAGCAGCCAGAGAGTCCTCTTTCAG AGAGCACATCTAACTCTGGCCTGCCGACTCCATCTCATTGGCCCAGGAAGGATCCAGTCTACCTGCCTCA CTTCTGAGTGCCTGGGCCATCCTGCTAGGGTCCACTGGAAAGGAAAAATGCCTTCTACAGAGATCATGGA CCACATCCTGAGGAAGGCACAGCTAGACTACATCGTCCCAAGAGTGCTGAACAACAGGCAGCTGAGAATG TCAATTTCATTTTGTGGAGGCATCGGGTTTGAGGGAATTCACCCAAGGCCACTATGGGATGAAGGTTATG AACTGGATGACCATACTGTGAGAGACTGAACTAGATTGGGTGTATGGCTGGCTGTCACTTTCTGACCTAG GTCAGTCTGTACTCTCTGTTCTCAAGGAACAGTGGCAGATGGGAGGAGAGCTGGGGCAATTCTTTCAGAT TGTGGTTTATATGGGAAATTGTTCATGGTCCCCAGCCTCTCAATGTATGTCTGTAGATGCATTGTGAACG CATGCTGCAGAAGGCCTTTGCTCATTTGAACTGTGTAGAGGCAAGAAGTGACCGGCTCATGTCAATGCTG TCTACAAACGAGTATGATCCTAACTGTTTTGGATAATCTTTTATATTTCTGAACTCTGAATTTAATCATT TTATTAGATTAAAATATGCGATTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191849 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191849 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006650 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source