EMAGE mouse gene expression spatial database

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EMAGE:11361

Prss1 protease, serine, 1 (trypsin 1) ( MGI:98839)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006656. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006656_01	euxassay_006656_02	euxassay_006656_03	euxassay_006656_04

euxassay_006656_05	euxassay_006656_06	euxassay_006656_07	euxassay_006656_08	euxassay_006656_09

euxassay_006656_10	euxassay_006656_11	euxassay_006656_12	euxassay_006656_13	euxassay_006656_14

euxassay_006656_15	euxassay_006656_16	euxassay_006656_18	euxassay_006656_19	euxassay_006656_20

euxassay_006656_21	euxassay_006656_22	euxassay_006656_23	euxassay_006656_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11361_wholemount_strong.wlz

11361_wholemount_moderate.wlz

11361_wholemount_weak.wlz

11361_wholemount_possible.wlz

11361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8165
Entity Detected:	Prss1, protease, serine, 1 (trypsin 1) ( MGI:98839)
Sequence:	sense strand is shown >T8165 CAATGAGTGCACTCCTGTTCCTGGCCCTTGTGGGAGCTGCTGTTGCTTTCCCTGTGGATGATGATGACAA GATCGTTGGAGGATACACCTGCCGAGAGAATTCTGTTCCCTACCAGGTGTCCCTGAACTCTGGCTACCAC TTCTGTGGAGGTTCCCTCATCAATGACCAGTGGGTGGTGTCTGCAGCTCATTGCTACAAGACCCGCATCC AAGTGAGATTGGGGGAGCACAATATCAATGTCCTGGAGGGCAATGAGCAGTTTATCGATGCTGCCAAGAT CATCAAGCACCCCAACTTCAATAGGAAGACCCTGAACAATGACATCATGTTGATCAAGCTCTCATCCCCT GTGACCCTCAATGCCAGAGTGGCCACTGTGGCTCTGCCCAGCTCCTGTGCACCTGCTGGCACTCAGTGTC TCATCTCTGGCTGGGGCAACACTCTTAGCTTTGGTGTCAGTGAACCAGACCTGCTCCAGTGCCTGGATGC CCCACTGCTGCCTCAGGCTGACTGTGAGGCTTCCTATCCTGGGAAGATCACTGGTAACATGGTCTGTGCT GGCTTCCTAGAGGGAGGCAAGGATTCCTGCCAGGGTGACTCTGGTGGCCCTGTGGTCTGCAATGGAGAGC TCCAGGGCATTGTCTCTTGGGGCTATGGCTGTGCCCTGCCAGACAATCCTGGTGTGTACACCAAGGTCTG CAACTATGTGGATTGGATTCAGGACACAATTGCTGCCAACTAGAGATTCCCAATCCCTCTGCAGTCACTG TCAATAAAGTGAAATATTCATTACTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6433981 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6433981 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11360 same embryo
	EurExpress:euxassay_006656 same experiment
	MGI:4827454 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source