EMAGE mouse gene expression spatial database

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EMAGE:11365

Gatsl3 GATS protein-like 3 ( MGI:1919212)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006785. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006785_01	euxassay_006785_02	euxassay_006785_03	euxassay_006785_04

euxassay_006785_05	euxassay_006785_06	euxassay_006785_07	euxassay_006785_08	euxassay_006785_09

euxassay_006785_10	euxassay_006785_11	euxassay_006785_12	euxassay_006785_13	euxassay_006785_14

euxassay_006785_15	euxassay_006785_16	euxassay_006785_17	euxassay_006785_18	euxassay_006785_19

euxassay_006785_20	euxassay_006785_21	euxassay_006785_22	euxassay_006785_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11365_wholemount_strong.wlz

11365_wholemount_moderate.wlz

11365_wholemount_weak.wlz

11365_wholemount_possible.wlz

11365_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11365_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 17 weak expression: see section 16

vibrissa

weak

regional

weak expression: see section 07 08 09 10 11 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8206
Entity Detected:	Gatsl3, GATS protein-like 3 ( MGI:1919212)
Sequence:	sense strand is shown >T8206 CGCCACCGCATCGCCTCCTGCCTAGGTCTCGCTGGCGGCCAGGCCGGGGCTGCCGTATCTGCGATGGAAC TTCACATCCTAGAGCACCGGGTGCGAGTACTGAGCATTGCCCGTCCGGGCCTCTGGCTCTACACCCACCC GCTCATCAAGCTGCTCTTCCTGCCCTGTCGCAGTCGGTGCAAGTTCTTCAGCCTGACTGAGACTCCGGAA GATTACACTCTCATGGTGGATGAGGAGGGCTTTAAAGAGCTACCCCCATCCGAGTTCCTGCAAGTGGCTG AGGCCACATGGCTGGTGATGAACGTATCTCACAGTGGCTCCGTGGTGCAGGCTGCTGGAGTAACCAAGAT TGCCCGGTCTGTCATTGCCCCGCTGGCTGAACACCATGTGTCTGTTCTGATGTTGTCCACATACCAGACA GACTTCATCCTGGTACGGGAGCAGGACCTGTCTGTGGTGATCCACACACTGGCCCAGGAGTTCCAAATCT ACCGTGAAGTAGGCGGGGAGCCTGTGCCCGTTACTGGAGACGATTCCAGCAATGGCTTTCCCCAGATCCA GCACGGGCCCAGTCCCACGGTGCATCCCATTCAGAGCCCTCAGAACCGCTTTTGTGTCCTCACACTTGAC CCTGAGACACTGCCGGCTGTCGCCACCACCCTCATCGATGTCCTCTTCTACTCCCACAGTGTCCCCAAGG AAGCGGCCTCGGGCGGTCCTGAATCAACGTCCATTCCGTTCTTCGCTTTCTCCCTCATCGAGGGGTACAT CTCCATTGTCATGGATGCAGAGATCCAGAGAAAGTTCCCCAGTGACCTCCTGCTGACCAGCTCTTCTGGG GAGCTGTGGAGGATGGTGCGCATCGGGGGACAGCCCCTGGGCTTCGATGAATGTGGGATTGTGGCCCAGA TTGCGGGTCCCCTGGCGGCTGTTGACATCTCTGCTTACTACATCAGCACATTCAACTTTGACCATGCGCT GGTTCCTGAAGACGAGATCGGCTGTGTCATTGATATTCTCCAGCGAAGGCAGGAAAGCCAGGCTTCCAAG GACCCATAGGGAAGCACCAGACCCCCAGCATTCCCGTAAAGCCCCCCGGGGCTCCAGAAACTTTTCTGAG CTTCAGAACAAGTCCCTGGTCCTGCCAAGGGATCCTGGTTCTGCTGTCTGTCTGTCAGCTGTATGGAGCT GGGACTCGTGGCCATGCCTGGTTCTGCTGTCTGTCTGTCAGCTGTATGGAGCTGGGACTCGTGGCCATGC CTGCCCACAAAAGATACCTTGGTGCTTGGGGCTTAGATCCCCGACCCAACATTTTCTATGTGACCTCAGC AATGCACAGAGCCTGCTTGCCTGCAGCTGGAGCCGCCTCTCCCCTGGCTAAGGCTCACATCCCCGGGCCG GGCTCTGACCTCCAGCCTGCACTGCTTTCCTAGCCAGATGAGTTCAGCAGTGCACTCCCTTCGCGGGACT CCGAAGGAAGAATTAAATCTCACTTTTATTTTCATGACTGTTTATATAAATAAAAGACTTGCGGATTTGT GCCCTCTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1195616 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1195616 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11366 same embryo
	EMAGE:11367 same embryo
	EMAGE:11369 same embryo
	EMAGE:11368 same embryo
	EurExpress:euxassay_006785 same experiment
	MGI:4825008 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source