EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11366

Ndufaf4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 ( MGI:1915743)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006782. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006782_01	euxassay_006782_02	euxassay_006782_03	euxassay_006782_04

euxassay_006782_05	euxassay_006782_06	euxassay_006782_07	euxassay_006782_08	euxassay_006782_09

euxassay_006782_10	euxassay_006782_11	euxassay_006782_12	euxassay_006782_13	euxassay_006782_14

euxassay_006782_15	euxassay_006782_16	euxassay_006782_17	euxassay_006782_18	euxassay_006782_19

euxassay_006782_20	euxassay_006782_21	euxassay_006782_22	euxassay_006782_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11366_wholemount_strong.wlz

11366_wholemount_moderate.wlz

11366_wholemount_weak.wlz

11366_wholemount_possible.wlz

11366_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11366_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 04 05 06 07 08 19 20 21 22 23

submandibular gland primordium

strong

regional

strong expression: see section 09 10 11 17 18 19 20 21 moderate expression: see section 08

vibrissa

moderate

regional

moderate expression: see section 09 10 11 22 23 weak expression: see section 08

esophagus epithelium

moderate

regional

moderate expression: see section 13

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23

metanephros

moderate

regional

moderate expression: see section 09 10 11 17 18 20 21 weak expression: see section 19

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8208
Entity Detected:	Ndufaf4, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 ( MGI:1915743)
Sequence:	sense strand is shown >T8208 GTGCCGGTGGCTTCTACACGTGCGTAGTGAACTAAGGGCATCATGATTTAGAGATGGGGGCTCGCGTGAC CCGCGCCTTAAGGAACTTCAACGTGGAGAAGCGAGCGGAGCGAGAGATCAGCAAGAGGAAGCCCTCCATG GCACCCAAGCACCCTTCTACCCGCGACCTCCTGCAGGAGCACCGGAGTCAGTATCCAGAAATCGAAGAAG TTGTTTCTAAAAAAGATAACAAGCTGCTGTCATTGCTAAGAGATGTATATGTCGATTCCAAAGATCCGGT GCCTGCCTTGCCGGTAAAAGTTGAACCACGACAAGAACCAAAGGAATTCAGACTGCCGATAGGAAATCAC TTTGATAAGAATATCACGGACATTCCCAAAGGCAAGATTACTGTTGTAGAGGCATTGACCCTTCTCAATA ACCATAAACTCTCTCCAGAAACATGGACTGCTGAGAAAATCGCTCAAGAGTACTATTTAGAACTGAAGGA TGTAAATTCCCTTCTCAAATATTTTGTTACTTTTGAAGTCAAAATCTTACCTCCAGAAGACAGGAAAGCA ATACAATCAAAATGAAGAAAATCACGAAACAACTTCCCCTGTATGTGTTCCCAAGCTCCGCGCCCTTGTT TTGCGTGTATTAAATTATACAAGTTCCTGTGAGTTGAGGGCCATAACGTTTGAAAGCTCTTTGACCTCCT CAGGATTGTTCATATAATTTTTTAAAAATTTATGTATGTGTAGTTCCTGGGTATATGTTAGCTGTATTGA TCATGTGGCCCAAATGAGTCAATATTAAATTCCACTTTCTAGTTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1195846 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1195846 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11365 same embryo
	EMAGE:11367 same embryo
	EMAGE:11369 same embryo
	EMAGE:11368 same embryo
	EurExpress:euxassay_006782 same experiment
	MGI:4826644 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source