EMAGE mouse gene expression spatial database

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EMAGE:11370

Parvg parvin, gamma ( MGI:2158329)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006776. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006776_01	euxassay_006776_02	euxassay_006776_03	euxassay_006776_04

euxassay_006776_05	euxassay_006776_06	euxassay_006776_07	euxassay_006776_08	euxassay_006776_09

euxassay_006776_10	euxassay_006776_11	euxassay_006776_12	euxassay_006776_13	euxassay_006776_14

euxassay_006776_15	euxassay_006776_16	euxassay_006776_17	euxassay_006776_18	euxassay_006776_19

euxassay_006776_20	euxassay_006776_21	euxassay_006776_22	euxassay_006776_23	euxassay_006776_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11370_wholemount_strong.wlz

11370_wholemount_moderate.wlz

11370_wholemount_weak.wlz

11370_wholemount_possible.wlz

11370_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11370_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

regional

strong expression: see section 13 14 15 16 17 18

liver

strong

spotted

strong expression: see section 09 10 13 14 moderate expression: see section 01 02 03 04 05 06 07 08 11 12 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7335
Entity Detected:	Parvg, parvin, gamma ( MGI:2158329)
Sequence:	sense strand is shown >T7335 GCGTCGGGAACCTGGTCACCCTCTAAGCTAGTGGGCTCCATTTCCTGATTTGAAAAGAAGCAGCGGGGAA GGCAAGGAAGAAGATGGAGTCAGAGTTCCTCTATGACCTGTTGCAGCTCCCAAAGGAGGTAGCTCAGCCC ACGGAGGAAGAGCTTCCACGAGGAGGAAAGAAGAAATATCTGTCACCCAATTCCAAGAGGAACCCCAAGT TTGAAGAACTGCAGAAGGTGCTGATGGAATGGATCAACACCACACTCCTCCCGGAGCACATCGTGGTCCG CAGCCTGGAGGAAGACATGTTTGATGGACTTATTCTGCACCACCTGTTCCAGAAGTTGGCATCGCTCAAG CTGGAAGTGGAGGAAATCTCTCTGACCTCGGCCAGCCAGAGGCACAAGCTGGGGGTCATCCTGGAGGCCG TCAACCAGAACCTACAGGTGGAAGAGAAGCAGGCCAAGTGGAGCGTGGAGACCATCTTCAACAAGGACCT GCTGGCCACCTTGCATCTCCTGGTGGCCCTGGCCAAACGCTTCCAGCCGGACCTGCCCCTCCCAGACAAC GTCCAGGTGGAAGTCATCCACATTGAGAGCACCAAGACCGGCCTGAAGTCGGACAAGCAGGTGGAGCAGC TCACAGAATGCAAGTCACACAAGGACCAGCCTTTACAGGACGCCTTTGATGAATTATTTAAGCTGGCTCC GGAGAAAGTGCACGCCGTCCAAGAGGCCATTGTGAGCTTTGTCAACCAGAAGCTAGAGCGCCTGGGCCTG TCTGTGCAGAGTCTGGATACCCAGTTTGCAGATGGCGTCATCCTGCTCCTGCTGATTGGACAACTGGAAG GCTTCTTCTTGCATCTGACGGAATTCTATCTCACTCCCAGCTCTCCCACAGAAATGCTGCACAATGTCAC CCTAGCCCTGGATCTGCTCAAGGATGAAGGCCTGTTCAGCTACCCTGTCAACCCTGAAGATATTGTGAAC AAGGACGCCAAGAGCACACTGAGGATACTTTACAGCCTATTCCAGAAGCATTCACTGAGGGCGGAGGGTG GAGGGGCACACCATGCTACCCCAAACTAGTCACCTCCACCTCCTCCAGCTGTTTCTCGGGAGCTGCCACC TCTGAAGGACCCAGCAGTCAGAGCTCTTCCCATGCCAGCCTTGTTACTGTGGCTTGGTGACATGTTTCCC TCCTATACCTTTGACCTCTGCTTACCTTTGACTCTACCAGATGGGGACCTATTTTGTGCCCACCCCTGCT TTGCTCCGTGCATTTTGACAGTGTATGAATTGCGTTTAAGTGGATGAGGGGCCCTGCATGCCTGTGAAGT CCTGGGTTAGCCTGTGGTGAAAAAGAGTTTGATTCCCAGGCGAGACCAGCCCTCATCAGCATCCAGTCTG TCTACTAAGGCTATACCTTGGTAGATAGTGGCTGCTCAGACTCCACAAAGCTGACCATCCCTAGCCAGAG GCTCTTTGGGGTTAAGGCTGATGGTCTCAGGCCATGGTCTCTATATACTTAGTTCCTGGCCATCTACCTT CCACCCCTCACTGGGGTGATGAGGCCAGGACTATCACCCAAAGACCAGAGCCCCATGTGGGAGAAAAGAG TTCAAAAGACCCTGGCCAGGCTATCCCTACAGCTGCTGTCCTGAGCTCAGCCCTGAGCCCCTGGGAAGAG AACCTGGAGTTGGTTCTTGGGTTCAGGTTAACCCTGGTCTGCATTCTATCCCGTGAGACTTGGTGTCTTC TGAAAAGAGAGAGGAACAGCTGAATAACTGAGCTGGGTCCATGGAGGAGGAGAAGTTGTGGGAGGAGATG CAAGTCATGGGGTTCAGGGGCACAGAGACATAGGGGTGCAGGGACTCAGGGGTGCAGGGATGTGGGGAGG TTAGCCTAGCTGTTCCGGTTCTGGACTTTGCCCTGCTTGCCCAGCTCTAGGTCCCTGCGAGGAACGTCTT TTAGGTTTGGGGGTATATAGCCGAACAGCTTTGCCTTTGGGGAATTTTGGAGCAGAGTCTGGCCAGGGCA GGCCACCCCTACAACCACAGTCGGTGGAGCTACGTGTTCTGGGGCTCTGGTGTCTGATGTGAGGAGGTCT GGGGCTTTTCTCTACCCAGCTCCATTCCACTTGTCCCTTTGGCCCTAGGCAGGCCACTCATAAGTGTTAG AGGGATATCTCCTGTCCTGTTACCTCTGGGACATGTCCCTCTTCCGTATCAGGGGCATCCTTATTGTAGC TGGGGTCCGGCTATCTCTTTCTGCTTAGGTAATCCTTTCATAGAGACCTTCTTCTGTGCCCCATCTCTTC TCTCTGGAGCATCCTGAGCCGTGATACAAGCTTGAGCCTCTCTGCTTCATTACTGGATGCTTGGCAGGTC AAAACCGGAGTCCACGCTCAACTCCTAGGCTCTTGGCTCTCCCTCCCTCTTTCTTCCCTGCTTGCTTGCC CTCTTGGCCCCTCCATGTCCTCACCTCCACCTCCTCACCTCCTGTCTCATGGGAGATTTGAATCATAAGG AGAATCACTTTTTGTAACTTTATTGAGGAATAAATAACATTTCCCAGTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4190510 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190510 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11374 same embryo
	EMAGE:11371 same embryo
	EMAGE:11373 same embryo
	EMAGE:11372 same embryo
	EurExpress:euxassay_006776 same experiment
	MGI:4827057 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source