EMAGE mouse gene expression spatial database

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EMAGE:11380

Upp1 uridine phosphorylase 1 ( MGI:1097668)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006779. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006779_01	euxassay_006779_02	euxassay_006779_03	euxassay_006779_04

euxassay_006779_05	euxassay_006779_06	euxassay_006779_07	euxassay_006779_08	euxassay_006779_09

euxassay_006779_10	euxassay_006779_11	euxassay_006779_12	euxassay_006779_13	euxassay_006779_14

euxassay_006779_15	euxassay_006779_16	euxassay_006779_17	euxassay_006779_18	euxassay_006779_19

euxassay_006779_20	euxassay_006779_21	euxassay_006779_22	euxassay_006779_23	euxassay_006779_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11380_wholemount_strong.wlz

11380_wholemount_moderate.wlz

11380_wholemount_weak.wlz

11380_wholemount_possible.wlz

11380_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11380_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8176
Entity Detected:	Upp1, uridine phosphorylase 1 ( MGI:1097668)
Sequence:	sense strand is shown >T8176 TTTACAACAGCCTAGCTCTGCATTCTTGACCACGTCCACACGGCCATTCATCTCTGGTCTTGACGAAGTG ATTGACTGGTGGTCTCTGACACTCTGGAAGCCTTCTCGCGACGTGGACATCGGTCTCTGACAGTCCTCCT ACAGTGAGAACATGGCAGCTACAGGAACTGAAGCAAAGGACCTTGAAAACCATCACAATGACTGCTTCAT CCAACTTTCAAATCCAAACATAGCAGCAATGAAGGAAGACGTGCTCTACCATTTCAACCTCAGCACTAGC ACACACGATTTCCCTGCTATGTTTGGAGACGTGAAGTTTGTGTGTGTTGGTGGAAGCTCTTCCCGGATGA ACACCTTCATCAAGTATGTGGCTGCAGAGCTGGGCCTTGACCATCCAGGGAAGGAATATCCCAACATCTG TGCAGGCACTGACCGCTACGCCATGTATAAAGCCGGGCCCGTGCTGTCTGTGAGCCATGGCATGGGCATT CCTTCCATCGGCATCATGTTGCACGAGCTCATCAAGATGCTGTACCATGCCAGGTGCTCCAACATCACCA TCATCCGCATTGGCACTTCAGGCGGGATAGGTCTGGAGCCTGGCTCTGTTGTCATCACGCAGCAGGCAGT GAATGAGTGCTTCAAGCCGGAGTTTGAGCAGATTGTCCTGGGAAAACGGGTGATTCGCAACACCAACCTG GACGCGCAGCTGGTTCAGGAGTTGGTGCAGTGCTCCTCAGACCTGAATGAGTTCCCCATGGTTGTGGGCA ACACCATGTGCACCTTGGACTTCTATGAGGGGCAAGGCCGTCTGGATGGCGCCCTCTGCTCCTACACAGA GAAAGACAAACAGTCTTATCTGCGTGCAGCCCATGCCGCGGGTGTCCGAAATATCGAGATGGAATCTTCG GTGTTTGCCACCATGTGCAGTGCCTGTGGCCTGAAAGCGGCTGTGGTGTGTGTCACCCTTCTGGACCGTC TGCAAGGAGACCAGATCAACACTCCCCACGATGTGCTTGTGGAATACCAGCAGAGACCACAGCGGCTAGT GGGCCACTTCATCAAGAAGAGCCTGGGACGGGCCTGAGCCCTGTGCTTTTCCGCCCCGGAGAAGCCTCTG CAACTCCTGGGCAATAAATCTATTTTTTCATAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5144694 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5144694 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11384 same embryo
	EMAGE:11385 same embryo
	EMAGE:11383 same embryo
	EMAGE:11382 same embryo
	EMAGE:11381 same embryo
	EurExpress:euxassay_006779 same experiment
	MGI:4829112 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source