EMAGE mouse gene expression spatial database

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EMAGE:11381

Tspan9 tetraspanin 9 ( MGI:1924558)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006763. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006763_01	euxassay_006763_02	euxassay_006763_03	euxassay_006763_04

euxassay_006763_05	euxassay_006763_06	euxassay_006763_07	euxassay_006763_08	euxassay_006763_09

euxassay_006763_10	euxassay_006763_11	euxassay_006763_12	euxassay_006763_13	euxassay_006763_14

euxassay_006763_15	euxassay_006763_16	euxassay_006763_17	euxassay_006763_18	euxassay_006763_19

euxassay_006763_20	euxassay_006763_21	euxassay_006763_22	euxassay_006763_23	euxassay_006763_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11381_wholemount_strong.wlz

11381_wholemount_moderate.wlz

11381_wholemount_weak.wlz

11381_wholemount_possible.wlz

11381_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11381_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 23 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01

hand

strong

regional

strong expression: see section 01 02 03 24

foot

strong

regional

strong expression: see section 05 06 07 22 23 24

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24

corpus striatum

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 20 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 06 07 09 10 11 12 13 14 16 17 18

pons mantle layer

moderate

single cell

moderate expression: see section 06 08 09 10 14 15

trigeminal v ganglion

strong

single cell

strong expression: see section 04 05 07 08 09 18 19 20 21 22

eye skeletal muscle

strong

regional

strong expression: see section 04 05 06 07 08

esophagus

strong

regional

strong expression: see section 08 09 10 11 12 13

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 moderate expression: see section 17

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

rectum

strong

regional

strong expression: see section 13 14

midgut

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20

midgut loop

strong

regional

strong expression: see section 10

bladder

strong

regional

strong expression: see section 12 13 14

tail paraxial mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

extraembryonic component

strong

regional

strong expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8178
Entity Detected:	Tspan9, tetraspanin 9 ( MGI:1924558)
Sequence:	sense strand is shown >T8178 GGCACGAGGGGGAGCGGAGCCGGGGCGCCACACCGAGTCCAAGCGCGCGTTGCAGCTGGCGAAGACTTGG GAAGTCAGATCCAAGCTGTCCCCTCTCAGAGGAGAGTGAGAGGATCCAGCTCAGGTGACCCAGGCAGGCA GTCAGAGAATTTAAGAAGTGCAACATGGCCAGGGGCTGTCTCTGCTGCTTGAAGTACACGATGTTCCTCT TCAATTTGATATTCTGGCTCTGTGGCTGTGGGCTGCTCGGAGTGGGCATCTGGCTCTCCGTGTCCCAAGG CAACTTCGCCACCTTCTCCCCCAGCTTCCCCTCACTGTCCGCAGCCAACCTCGTCATCGCCATCGGCACC ATCGTCATGGTGACGGGCTTCCTGGGCTGCCTGGGGGCCATCAAGGAGAACAAGTGTCTGCTTCTCAGCT TCTTCATCGTGCTGTTGATCATCCTGCTAGCAGAGTTGATTCTGATCATCCTCTTCTTCGTCTACATGGA CAAGGTGAACGAGAATGCCAAGCAGGACCTGAAAGAGGGGCTGCTGCTCTACAACACAGAGAACAACGTG GGGCTCAAGAACGCCTGGAACATCATCCAGGCCGAGATGCGGTGTTGTGGGGTCACTGACTACACAGACT GGTATCCGGTGCTCGGGGAGAACACAGTTCCTGACAGATGCTGCATGGAGAATTCCCAGGGCTGTGGGCG CAACAGCACCACCCCCTTGTGGAGAACGGGCTGCTACGAAAAAGTGAAGCTGTGGTTTGATGACAACAAG CACGTGCTAGGCACCGTGGGCATGTGCATCCTCATCATGCAGATCCTGGGAATGGCCTTTTCCATGACAC TGTTCCAGCACATCCACCGGACAGGTAAAAAGTACGACGCCTGAGGCTGCGGCAGCGCCTGGCTCCACCC GGACACTGCGTGTGCCAGGGAAGAGGACCCGAGTCATGTCTCCTGCCTGCTCTCCGATGGCCACCCTGCA CCACCTGCCGGCCTGCCCTCCCCTGCCCACATCCTTGGCCTTAGACCTCATGGAGGGCAGAGGCCCAGGG ATGAGGCATCATGCAGAGACCTCAGGGCTTGGGCAACTGGATTCCTGCACCTGCAGACTTGCCAAAGATG CCGGGGCGGGCAGGGTGTCAGCGAGGAAGCCCCTTCAACGATGGGTTTTTGCCCCTGGCCTTCCTCACAC TGACACTTGTTCCTATGTGGGGTGGAGAGTCTGCCCCATGGAGGCCACTGCCGTCCATGGCTGCCCAGCC AGAGAGCCTGCCAGGGTGCAGTGTGGCACCGCCAGGTCAGGCCCCCTGGAGCACCCTGCCCGGGTTTTTA TACTATAGTGATAACTTTTTTTTTTATTTTACTCTGATTATGATTATTCAGGAATATTATCTCTCAGATA AGTTTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5146854 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5146854 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11384 same embryo
	EMAGE:11385 same embryo
	EMAGE:11383 same embryo
	EMAGE:11382 same embryo
	EMAGE:11380 same embryo
	EurExpress:euxassay_006763 same experiment
	MGI:4828971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source