EMAGE mouse gene expression spatial database

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EMAGE:11384

Rnf208 ring finger protein 208 ( MGI:1916096)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006729. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006729_01	euxassay_006729_02	euxassay_006729_03	euxassay_006729_04

euxassay_006729_05	euxassay_006729_06	euxassay_006729_07	euxassay_006729_08	euxassay_006729_09

euxassay_006729_10	euxassay_006729_11	euxassay_006729_12	euxassay_006729_13	euxassay_006729_14

euxassay_006729_15	euxassay_006729_16	euxassay_006729_17	euxassay_006729_18	euxassay_006729_19

euxassay_006729_20	euxassay_006729_21	euxassay_006729_22	euxassay_006729_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11384_wholemount_strong.wlz

11384_wholemount_moderate.wlz

11384_wholemount_weak.wlz

11384_wholemount_possible.wlz

11384_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11384_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 16 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 10 17

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 15 16

neural retina

moderate

regional

moderate expression: see section 02 03 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8181
Entity Detected:	Rnf208, ring finger protein 208 ( MGI:1916096)
Sequence:	sense strand is shown >T8181 GGGACTGCTGACCTCTGCTACGGAGGAGGCCCCTCCACGCCCCCTTGCCAGCTTCAGCAGACAGAGACCC TGGGCCTGGCAGGACCAGGATGCAGCCCCTTCTCTTCCCCCACGGCCCCACCCAACACCCCCACCTGTGC CAACGTCCAGTCTGACTGTAGAGAGCCGGATGCCAGCTGACCCTGGGCCTGAGGTGGGCAGTGGATGGCC GGGCCTCCTCATGTCCTGCCTGAAAGGCCCCCATGTCATCCTCAAAATGGAGGCCATGAAGATTGTCCAC CCTGAAAAGTTCCCTGAGCTCCCGGCGGCCACCCCCTGCTTCCCTCCTGCACCCAGGCCCACTCCCACTC TGGCACCCAAGCGTGCTTGGCCCTCAGACACAGAGATCATTGTCAACCAGGCATGTGGGGGGGACATGCC TACCTTGGAAGGAGCATCCCACACTCCACCCTTGCCAAGGCGGCCCCGCAAAGGCAGTTCTGAGTTGGGC TTCCCCCGGGTGGCACCAGTGGATGAGGTCATTGTAAATCAGTATGTGATTCGACCTGGCCCAACAGCCT CTGCACCTTCATCGTCTGGGCCAGTGATAGCAGGTGAGCCCCTGGAATGCCCCACCTGTGGGCATACATA CAACGTCACTCAACGGCGGCCCCGTGTGCTGTCTTGCCTGCACTCCGTGTGTGAGCAGTGCCTGCAGATT CTTTATGAGTCTTGCCCTAAGTACAAGTTCATCTCCTGTCCCACCTGCCACCGTGAGACTGTGCTCTTCA CTGACTATGGCCTGGCTGCACTGGCTGTCAACACATCCATCCTGAGCCGCTTGCCACCTGAAGCCCTGAC TGCCCCATCCGGTGGACAGTGGGGGGGTGAATCTGAAGGCAGCTGCTACCAGACCTTCCGGCAGTACTGT GGAGCTGCATGCACCTGCCATGTGCGGAACCCACTATCTGCCTGTTCCATCATGTAGTGTCCACCTGCCC ACTACTGCCTGCAGGTCCTTGCTCGCCGCTTCTTCAGGGAACTGGCTTTGTCCTGTTGCCCGCTGACCCT TTCTTGCCCACTGTGGCTTCTGGCCCTACCCTGCGTGGCATTGTCGCTGCAGCCAACTTTGCCATTAAAA CTCTTTGCCAAAGTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5254639 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5254639 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11385 same embryo
	EMAGE:11383 same embryo
	EMAGE:11382 same embryo
	EMAGE:11381 same embryo
	EMAGE:11380 same embryo
	EurExpress:euxassay_006729 same experiment
	MGI:4827772 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source