EMAGE mouse gene expression spatial database

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EMAGE:11390

Ap2a2 adaptor protein complex AP-2, alpha 2 subunit ( MGI:101920)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008246. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008246_01	euxassay_008246_02	euxassay_008246_03	euxassay_008246_04

euxassay_008246_05	euxassay_008246_06	euxassay_008246_07	euxassay_008246_08	euxassay_008246_09

euxassay_008246_10	euxassay_008246_11	euxassay_008246_12	euxassay_008246_13	euxassay_008246_14

euxassay_008246_15	euxassay_008246_16	euxassay_008246_17	euxassay_008246_18	euxassay_008246_19

euxassay_008246_20	euxassay_008246_21	euxassay_008246_22	euxassay_008246_23	euxassay_008246_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11390_wholemount_strong.wlz

11390_wholemount_moderate.wlz

11390_wholemount_weak.wlz

11390_wholemount_possible.wlz

11390_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11390_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9131
Entity Detected:	Ap2a2, adaptor protein complex AP-2, alpha 2 subunit ( MGI:101920)
Sequence:	sense strand is shown >T9131 AGCGGCGCTCGGGGGCCCGAGGCGGCTGTGGCTAGGCGGCTCCGCGCTCCAGGGCTCTTCGGCGTGACCG CCGCGCCCGTGCTGTTCTAGCCCGCCGCCGAGGCCGCTCCCGGGGCGCAAAGATGCCGGCCGTATCCAAG GGGGACGGGATGCGAGGCCTAGCGGTCTTCATCTCCGACATCCGCAACTGTAAAAGTAAAGAAGCTGAAA TAAAGAGAATAAACAAAGAACTGGCAAATATTAGATCCAAATTTAAAGGTGACAACGCTCTTGATGGCTA CAGTAAAAAGAAGTATGTCTGCAAATTGCTCTTCATCTTTCTCCTTGGTCATGACATTGACTTTGGACAC ATGGAAGCTGTGAATCTTCTGAGCTCAAACAGATACACGGAAAAGCAGATTGGCTACCTTTTCATCTCTG TATTGGTGAACTCGAATAGTGAACTGATCCGGTTGATTAACAATGCCATCAAGAATGACCTGGCCAGCCG CAATCCTACGTTTATGGGTCTCGCCCTGCACTGCATTGCCAATGTGGGTAGCCGTGAGATGGCGGAGGCC TTCGCCGGAGAGATCCCCAAGATCCTCGTGGCAGGAGATACCATGGATAGTGTGAAGCAGAGCGCAGCCC TGTGCTTGTTACGCCTGTACAGGACATCACCTGACCTAGTTCCCATGGGCGACTGGACATCTCGTGTGGT ACACCTCCTCAATGACCAGCACTTGGGGGTAGTGACTGCAGCGACTAGCCTCATCACCACCCTGGCCCAG AAGAACCCTGAGGAGTTCAAGACCTCTGTCTCGCTAGCTGTCTCGCGACTGAGCAGGATTGTGACGTCTG CATCAACAGATCTTCAGGATTATACTTACTACTTTGTCCCGGCTCCTTGGCTGTCAGTCAAGCTTCTGAG ACTGCTGCAGTGCTACCCACCCCCAGACCCTGCGGTGCGTGGCCGCCTGACTGAGTGCTTGGAGACCATC CTGAACAAGGCCCAGGAGCCACCCAAGTCCAAGAAGGTCCAGCATTCAAATGCCAAGAACGCTGTGCTGT TTGAGGCCATCAGCCTGATCATCCACCATGACAGTGAGCCAAACTTGCTCGTCCGCGCCTGCAACCAGCT GGGCCAGTTCCTGCAGCACCGGGAGACGAACCTACGCTACCTGGCTCTGGAGAGCATGTGCACGCTGGCC AGCTCTGAGTTCTCCCACGAGGCCGTCAAGACCCACATTGAGACGGTCATCAATGCCTTGAAGACGGAAA GAGACGTGAGTGTGAGGCAGCGGGCAGTGGACCTGCTCTACGCCATGTGTGACCGGAGCAACGCCCAGCA GATTGTGGCTGAGATGCTGAGCTACCTAGAAACGGCTGACTACTCCATCCGAGAGGAAATTGTGCTGAAG GTGGCCATCCTGGCTGAGAAGTATGCAGTGGACTACACTTGGTATGTGGACACCATCCTCAACCTCATCC GCATCGCGGGCGACTATGTGAGCGAGGAGGTGTGGTACCGCGTCATCCAGATTGTCATCAATCGCGATGA TGTGCAGGGCTATGCTGCCAAGACGGTATTTGAGGCATTGCAGGCGCCAGCATGTCATGAGAACTTGGTC AAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGGGACCCACGATCCAGCCCTCTGA TCCAGTTCAACCTGCTCCACTCCAAGTTCCACTTGTGCAGCGTCCCCACTCGGGCACTCCTGCTGTCCAC CTACATCAAGTTCGTGAACCTCTTTCCAGAGGTAAAAGCTACCATTCAGGACGTGCTGCGCAGTGACAGC CAGCTGAAGAATGCCGACGTGGAGCTGCAGCAGCGGGCTGTGGAGTACCTGCGACTGAGCACTGTCGCCA GCACTGACATCCTGGCAACTGTCCTGGAGGAAATGCCACCCTTCCCTGAGCGTGAGTCCTCCATCTTGGC CAAGCTGAAGAAGAAGAAGGGCCCAAGCACAGTGACTGACCTGGAGGAGACCAAGCGGGAGCGAAGCATC GATGTGAATGGGGGCCCTGAACCCGTTCCAGCCAGCACCAGTGCTGCGTCCACACCTTCTCCGTCAGCAG ACCTGCTGGGTCTGGGGGCCGTGCCCCCTGCTCCCACGGGGCCCCCTCCCTCCTCTGGCGGCGGGCTGCT GGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTCGCACCTCTTGCACCCGGCTCTGAAGACAACTTTGCC AGGTTTGTTTGTAAAAATAATGGTGTATTGTTCGAAAACCAGCTGCTTCAAATTGGACTTAAGTCAGAAT TTCGGCAGAATTTAGGCCGAATGTTCATATTTTATGGTAACAAGACCTCCACGCAGTTCCTAAACTTCAC TCCAACACTAATCTGTGCTGACGACCTTCAAACTAATCTGAACCTGCAGACTAAGCCCGTGGACCCAACT GTGGATGGGGGCGCACAGGTGCAACAAGTGGTCAACATTGAGTGTATATCTGATTTCACAGAGGCACCTG TCCTCAACATCCAGTTCAGGTATGGTGGGACCTTCCAGAATGTGTCTGTTAAGCTTCCCATCACTCTCAA CAAATTTTTCCAGCCCACAGAAATGGCTTCTCAGGATTTCTTTCAACGTTGGAAGCAGTTGAGCAATCCA CAGCAAGAAGTGCAGAATATCTTCAAAGCAAAGCATCCGATGGACACAGAGATCACTAAGGCAAAGATTA TTGGATTTGGTTCTGCGCTCCTGGAAGAAGTTGACCCGAATCCTGCAAATTTCGTGGGTGCTGGCATAAT ACACACCAAAACCACCCAGATTGGCTGCTTGTTGCGCCTGGAGCCAAACCTGCAAGCTCAGATGTATAGA CTCACCTTGCGTACCAGCAAAGACACCGTCTCTCAGAGACTATGTGAATTGCTGTCGGAACAGTTCTAGT CCGCAGACCAGAAGATCAGGCTCTTTGTTTGTGCGTCTCTCTTGTCTCTCTCGTTTGTCTTTGTACCACA CTGCAGATAAACACCCCTTGTCTGAAAACTGCCATTCACGGCACCTCCAGGCCTATGCCCACAGCCAGCC CCTGGGTGTGGAGGGAATGCAGGACTCAGTTTGAGAAGGGCTGCAGCAGGCCTGCCCCGTCAAAGAAGGG TGCCTGAGCCTGGGAAATCAGGGCAGTCCTGTGGTTCAACCTACAAATTAAAGGGAAATTAGAAGTTTGA ACGAAAGTGGAAATTTTGTTCAAATTTTATTAAAATGCCACTGGCTCAATCTCAGAATCCAGAAACAGCG CCATGTGCTTTTAGGGTTAACCCAGGGCTGCGTGGCTCTGCTGGATGGTGACAGCGGTGGCTTAGAGGTA TGTGACCTCATGGGCTAGGCAGTGTCCATGGAGTTTGCTGGAGCACTGCCTGGTGTATAACTGTTTGAAA CTCCATGCCCCACGCATGGCAGGGTCCCGTGCCTAACAAGCCCAGTGTTCTTGGAGGGACAGTTGAAACA TTTTACCACTTTTTTGAGATCTTAGGTTTGCAGCATGGTGGGCAATTCCTCTTGACTTTGGAAGACATGG ATATCACCCATCTTTGTTTTTAATGAATGTCCTATCCCTATGGTACATTCTGGGGACCTGAAAGCTAAGT GGGCGTGGGGTGGTAAGGTAATGACTGTGAGACATGCTTCTCTCCAGAGGGTCTTGATTGGTGCCCAGTG TTACATCCTCACTTCTGTGAAGCCCTTGTGGCTGGGTGGAAAAGAGAGACCTAAGTGTGGCAGTGGAGTG ACAGTCTGGGGCGAGAATGGCAACCCAGGCAGGTGCTGCGCTAAGGATGCACTGGACTCCCTAGCTCATG GGCCATTTAACCCTTTGTGGGCATTGGACTGTATGTACGGCCCATCAGGGGAAGAACATAGGCAGCGTCA GGAGCTCAGGCTTGGGGCCTTGCACTATGTCTTGCAGTAATGCCCAGGATTGCTATAGACTGAGCTGTAT GGGGTCATCTGTACGTACACAGCAGTGTTGTGTCCCTTGCCCACTGGAGCACTAGGTGTGGTCAGTTATG CACGGGTCTTTTTTTTTTTTTTTTTTGCCTCAGCCTCAGGCTGCCAGCCTGCCATGTGGGTTTGAGGACT GCAGCAGGGCTCTAACTACTCAAGCTCCCGCAGCCCCACTCTGTATAGCCCAGGCAGGTAGTTTCCGAAC ACCACCACCTGTGTGCTGATCCTTGCGTTCCACGAAGCCCTTGGTCTGCCTATCGTGTTGTGACCCACAC TGCCATGGCACAGGCTTAGGTTCCAGATGATCTAGCTGTGGCCACAATGAGCCTCCGAATCCCCTTGCTC CGTAGCTGTCCATTGTGTGCAGTCCACGCAGTATTGCATGGACCACGTGGGTAGCTCAACCTGTGTGGCT GTGGCTTGTGTACCACGTGCTGCCCAGTGATGGTGTCTGTATCAGTAATGTCCCAGCCCACGGGAGCTGC TGGGGCACAGGCCCACTCTCCGTGACTACCGGGCCTCCATTAGTCCAGTGTGCTTGTTCTCAGAGGAAAA AAAAAATAAATGCTAGGACAGGAAACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825406 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825406 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11389 same embryo
	EMAGE:11388 same embryo
	EMAGE:11387 same embryo
	EMAGE:11386 same embryo
	EurExpress:euxassay_008246 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source