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EMAGE:11400

Cpne8 copine VIII ( MGI:1914121)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400
"Pseudo-wholemount" of euxassay_008572. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008572_01 euxassay_008572_02 euxassay_008572_03 euxassay_008572_04
EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400
euxassay_008572_05 euxassay_008572_06 euxassay_008572_07 euxassay_008572_08 euxassay_008572_09
EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400
euxassay_008572_10 euxassay_008572_11 euxassay_008572_12 euxassay_008572_13 euxassay_008572_14
EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400
euxassay_008572_15 euxassay_008572_16 euxassay_008572_17 euxassay_008572_18 euxassay_008572_19
EMAGE:11400 EMAGE:11400 EMAGE:11400 EMAGE:11400
euxassay_008572_20 euxassay_008572_21 euxassay_008572_22 euxassay_008572_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11400Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11400_wholemount_strong.wlz
11400_wholemount_moderate.wlz
11400_wholemount_weak.wlz
11400_wholemount_possible.wlz
11400_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11400_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36194
Entity Detected:Cpne8, copine VIII ( MGI:1914121)
Sequence:sense strand is shown

>T36194
ACCCTTACCAACTGAATGCCTATGGAATGGCACTGAAGGCTGTAGGAGAAATCGTTCAAGATTATGACAG
TGATAAGATGTTCCCAGCCCTGGGTTTTGGTGCAAAGCTGCCTCCAGATGGAAGGATATCTCACGAGTTT
GCCCTGAATGGCAACCCTCAGAATCCTTACTGTGATGGCATTGAAGGTGTCATGGAAGCTTACTATAGAA
GTCTGAAGTCTGTCCAGTTGTATGGGCCAACCAACTTTGCTCCCGTAATAAACCACGTAGCAAGATACGC
ATCCTCTGTAAAAGATGGCTCCCAGTACTTTGTGCTTCTCATTGTGACGGACGGCGTCATCTCAGATATG
GCTCAAACTAAAGAATCCATAGTTAATGCCTCAAAGCTTCCCATGTCAATAATAATAGTAGGTGTGGGAC
CTGCAGAATTTGATGCAATGGTTGAATTGGATGGAGATGATGTAAGAGTTTCCTCCAGAGGGAAATATGC
TGAAAGAGATATTGTACAGTTTGTGCCGTTCAGGGATTATATTGACAGAAGTGGAAACCACATACTCAGC
ATGGCGAGACTGGCCAAAGATGTCTTAGCTGAGATCCCAGAGCAGTTCCTTTCCTACATGAGAGCCAGAG
GAATCAAGCCATCACCGGCGCCCCCGCCGTACACCCCTCCTACACACGTGCTGCAGACGCAAATATGACC
ACGCCCAGATGCTGATGTCCCTCACACGCCAGTCGGAACTGCGAAGCCTGTGCTTTGATCCTGGTGCTCT
GCTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63900. Forward Primer - name:063900_F_cDNA_Cpne8, sequence:ACCCTTACCAACTGAATGCCTA; Reverse Primer - name:063900_N_SP6_cDNA_Cpne8, sequence:AAAGCAGAGCACCAGGATCAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11397 same embryo
 EMAGE:11399 same embryo
 EMAGE:11398 same embryo
 EurExpress:euxassay_008572 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS