EMAGE mouse gene expression spatial database

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EMAGE:11401

Dido1 death inducer-obliterator 1 ( MGI:1344352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006747. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006747_01	euxassay_006747_02	euxassay_006747_03	euxassay_006747_04

euxassay_006747_05	euxassay_006747_06	euxassay_006747_07	euxassay_006747_08	euxassay_006747_09

euxassay_006747_10	euxassay_006747_11	euxassay_006747_12	euxassay_006747_13	euxassay_006747_14

euxassay_006747_15	euxassay_006747_16	euxassay_006747_17	euxassay_006747_18	euxassay_006747_19

euxassay_006747_20	euxassay_006747_21	euxassay_006747_22	euxassay_006747_23	euxassay_006747_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11401_wholemount_strong.wlz

11401_wholemount_moderate.wlz

11401_wholemount_weak.wlz

11401_wholemount_possible.wlz

11401_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11401_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8185
Entity Detected:	Dido1, death inducer-obliterator 1 ( MGI:1344352)
Sequence:	sense strand is shown >T8185 GCACGAGGGGCTGCTGCGGGGGCATTCGCGTACTGAGTGCTAGCGCGGGGATGGAGACGTGTAGACTGAG TTCGAAACGAAGGAGCTTGTTTAAGAGGCAGTCCCCACTGTCCCTTTGGTTGTTCGAAGCTCCGGAATCT TCTCATTGATGAACTGTGACTGACAACAGTGGGGTGAGGCTTGGCCGTCTGCTTGACCTGGCCCCAGGTC TATAATTTTATGTAGGATTTCCAGCCAAAGGTTTCCAAGCTTTCAGTTTTGGGACAGGTATGGATGATAA AGGGCACCTGAGCAATGAGGAAGCACCCAAGGCTATCAAACCCACCAGTAAGGAGTTCAGGAAAACCTGG GGTTTTCGAAGAACCACGATTGCCAAACGTGAGGGTGCAGGAGACACGGAGGTGGACCCCAGTGAGCAGC AACCACAGCAGCATAACCTCTCCCTGCGCCGCAGTGGACGGCAACCAAAACGTACTGAGAGGGTAGAAGA GTTTCTTACCACGGTTCGGCGCCGAGGGAAAAAGAATGTGCCGGTGTCCCTGGAGGATTCCAGTGAGCCC ACATCTTCCACAGTCACTGATGTGGAGACAGCTTCCGAGGGGAGCGTTGAAAGCAGTTCTGAGATCAGAA GTGGCCCTGTATCTGACTCCTTAGGGAAAGAACATCCTGCCTCTTCTGAAAAGGCAAAAGGAGGTGAAGA GGAAGAAGACACCTCTGACAGTGACAGTGATGGCCTTACGTTGAAGGAACTTCAGAACCGCCTTCGGAGA AAGCGAGAGCAAGAACCTGTGGAGAGGTCCCTGAGAGGCAGTCAGAATCGCCTGAGGAAGAAGCGCAGAG AGGAAGATTCTGCCGAAACTGGGAGTGTCCAAATAGGCAGTGCCGAGCAGGACAGACCTCTCTGTAAGCA GGAGCCTGAGGCTAGTCAGGGACCAGTGTCCCAGTCAGAGACAGATGACATAGAAAATCAGTTGGAAGGG AAGGCGACTCAGGGAAATACAGAGGAAAACCCCAGGGAAGCGGGCAAACCAAAGCCTGAGTGTGAGGTTT ACGACCCCAATGCCCTGTACTGCATCTGCCGCCAGCCTCACAACAACAGGTTTATGATCTGCTGTGATCG GTGTGAGGAGTGGTTCCATGGTGACTGTGTGGGTATTTCTGAGGCCCGAGGGCGGCTCCTGGAAAGGAAC GGGGAAGACTACATCTGCCCAAATTGCACCATTTTGCAAGTGCAGGATGAGACAAACGGTAGCGCCACCG ATGAGCAGGACTCTGGGTGCAGATCTGTGGGTGCTGATGGCACAGACTGCACAAGCATAGGGACAGTAGA GCAGAAGTCCGGAGAAGACCAGGGCATAAAGGGTAGGATTGAGAAGGCAGCAAACCCCAGCGGCAAGAAA AAACTCAAGATATTCCAGCCTGTCGTAGAGGCTCCTGGTGCTCCTAAATGCATTGGCCCTGGGTGTTCCA GTGTAGCACAGCCTGACTCTGTGTATTGCAGTAATGACTGCATTCTCAAACACGCAGCAGCTACCATGAG ATTTCTAAGTTCAGGTAAAGAACAAAAAACAAAACCCAAGGAAAAGGTCAAGACGAAGCCAGAAAAGTTC AGTCTTCCAAAATGCAGTGTTCAGGTGGGGATTAAAATCTCTTCTGTGCACAAGAGACTAGCGTCAGAGA AAAGGGAAAACCCAGTGAAGAAAGTGATGCTGGCTTCCAGGAGTGAGACTTCTGGGAAGGAGGCAGCCTG TGAGAGCAGCACACCATCCTGGGCAAGTGACCACAACTACAATGCTGTGAAGCCAGAGAAGCCAGAGAAG CCCACTGCACTCTCGCCCACCCTATTGAGTAAATGTACGTATCACCCAAAGGCTGGCTTCCCAGGCCCCT CCCATCATCTGGGTGGCTGCCTGGGGCTGTCTAGGACCAGAGTCCTGGGTGTTCTGGTGCTGATAGTAGC CAGCAGCTCACTGCCAGCCAGAAGCAGATACCAAGATGCCTCTGGACCCCAGGTGTTCCTGCCTAGCCTG TGGAGCCTCTCTGGGTGGTTCCTAAAGAGCTGTGTAGGCCTCATGTTGGAGGCAATTTCTTATTTCAGTT TTAGGCCTTGGTAAGTATTTGAACTGCTCTCAACAAGATGGGACAACATCAGTGCACTGTTCAGACTCAG TTCAGACTTGAGTTCCTCACAGGACAGCCCAGCACAAACCATGAGTTTTCCCACGTTACCTATTTTCTTC ACATGGTCACCACTGCTCTTCTATAGAAAAGTGATTTTTTTCATGAGTTAGGGCATTTTTGAAAGGACAG GTAGACAATTTGTGTCTATTTCACATACTACACACCTACATAAACAGGTTTGAATTTTGAAGGTCATCTG CTGCTCAGATCCCATGGTGAGTAATCGTGCTGACCATACACCTACAGCAGTATTCTCATCAAAGTGAGAA TTGTGGGTGGTAGCTTCCAGACTCGTAGATGCCCCAGATTTCAAATCAGTGTTTATAGTTTGAGTAAACT TTTGAAACCCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:832364 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:832364 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11405 same embryo
	EMAGE:11404 same embryo
	EMAGE:11402 same embryo
	EMAGE:11403 same embryo
	EMAGE:11406 same embryo
	EurExpress:euxassay_006747 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source