EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11405

Dusp23 dual specificity phosphatase 23 ( MGI:1915690)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006717. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006717_01	euxassay_006717_02	euxassay_006717_03	euxassay_006717_04

euxassay_006717_05	euxassay_006717_06	euxassay_006717_07	euxassay_006717_08	euxassay_006717_09

euxassay_006717_10	euxassay_006717_11	euxassay_006717_12	euxassay_006717_13	euxassay_006717_14

euxassay_006717_15	euxassay_006717_16	euxassay_006717_17	euxassay_006717_18	euxassay_006717_19

euxassay_006717_20	euxassay_006717_21	euxassay_006717_22	euxassay_006717_23	euxassay_006717_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11405_wholemount_strong.wlz

11405_wholemount_moderate.wlz

11405_wholemount_weak.wlz

11405_wholemount_possible.wlz

11405_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11405_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8186
Entity Detected:	Dusp23, dual specificity phosphatase 23 ( MGI:1915690)
Sequence:	sense strand is shown >T8186 TCCAGCCCCAGAGGGGGGGTGACCCGATCTCCCGGCAGAGTAGGAAAGGCCAGCCTCGCCCTGAGTAAAC TCCCCCCACACACACGATGGGCGTGCAACCCCCCAACTTCTCCTGGGTGCTTCCGGGACGGCTGGCCGGG CTGGCGTTGCCCCGGCTGCCCGCGCACTACCAGTTCCTGCTGGACCAGGGTGTGCGGCACCTGGTGTCCC TGACGGAGCGCGGACCCCCTCACAGTGACAGCTGTCCCGGCCTCACGCTGCACCGAATGCGCATCCCTGA CTTTTGCCCGCCGTCCCCGGAACAGATCGACCAATTTGTGAAGATCGTGGACGAGGCCAATGCCCGGGGA GAGGCTGTTGGAGTGCACTGCGCCCTAGGCTTTGGCCGCACTGGCACCATGCTAGCCTGCTACTTGGTGA AGGAGCGGGCTTTGGCCGCAGGAGATGCCATTGCTGAGATCCGGCGCCTGCGACCAGGATCCATTGAGAC GTATGAACAGGAGAAGGCCGTCTTCCAGTTCTACCAGCGAACAAAATGAGGACTTCAACAGCCCGCCTTT CCCCCTCCCCGACTCCTGCGGCCAGGGAGGAAGGGGAGTGAACTAAAGTACTGCATCCTTCAGGTCCCTC TGACTCCTATTGGACAAAAGTAGTCCTTCCCCAAAGCCATAATGTGGCCGGCAGGATGGCCGAGACCCCA CAAAAATGAGGTAATAACTGATAAGAACTCATCACCGCTGCATAGCATGTACACAGCACTCCCAATACAT CTGGGTGGTTGAAAAGACAAAAAAAAAAAAAAAAAAAAAACCATT
Notes:	The probe template was PCR amplified from IMAGE:850475 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:850475 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11404 same embryo
	EMAGE:11402 same embryo
	EMAGE:11401 same embryo
	EMAGE:11403 same embryo
	EMAGE:11406 same embryo
	EurExpress:euxassay_006717 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source