EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11415

Pstpip1 proline-serine-threonine phosphatase-interacting protein 1 ( MGI:1321396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006607. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006607_01	euxassay_006607_02	euxassay_006607_03	euxassay_006607_04

euxassay_006607_05	euxassay_006607_06	euxassay_006607_07	euxassay_006607_08	euxassay_006607_09

euxassay_006607_10	euxassay_006607_11	euxassay_006607_12	euxassay_006607_13	euxassay_006607_14

euxassay_006607_15	euxassay_006607_16	euxassay_006607_17	euxassay_006607_18	euxassay_006607_19

euxassay_006607_20	euxassay_006607_21	euxassay_006607_22	euxassay_006607_23	euxassay_006607_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11415_wholemount_strong.wlz

11415_wholemount_moderate.wlz

11415_wholemount_weak.wlz

11415_wholemount_possible.wlz

11415_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11415_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 10 11 12 13 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8152
Entity Detected:	Pstpip1, proline-serine-threonine phosphatase-interacting protein 1 ( MGI:1321396)
Sequence:	sense strand is shown >T8152 CCAAACAAAACAGGTTGAGCCTTTTTCCTCCTCCGGCAGTTGCCTCTGGCTTGTGGCTGCCTTCTGAGCG TTTCAGACGGCGCCGGCTGGGAGTGGGAGGGAGGGCCTGGGCTAGCCGCGCTGGGACTGGGACGTGCTCC TGGCTCCTGGCCCATGTTCAGCCCTGCTTGAAGCAGGAGTGCTAGCATTTGACACAACAGACGCCCTTGG AGGATGATGGCCCAGCTGCAGTTCCGAGATGCCTTCTGGTGCAGGGACTTCACGGCCCACACAGGGTATG AGGTGCTACTGCAGAGGCTGCTGGACGGCAGGAAGATGTGCAAGGATGTGGAGGAGCTGCTCAGACAGAG GGCCCAGGCGGAGGAGAGGTACGGGAAGGAGCTGGTGCAGATTGCACGCAAGGCTGGTGGCCAGACAGAG ATGAATTCCCTGAGGACCTCCTTTGACTCCCTGAAGCAGCAAACAGAGAATGTGGGCAGTGCACACATCC AGCTGGCCCTGGCCCTGCGTGAGGAGCTGCGGAGCCTGGAGGAGTTCCGAGAGAGACAGAAAGAGCAGCG GAAGAAGTATGAGGCCATCATGGACCGTGTCCAGAAGAGCAAGTTGTCGCTCTACAAGAAGACCATGGAG TCCAAGAAGGCATATGACCAGAAGTGCAGGGATGCAGATGATGCTGAGCAGGCCTTCGAGCGTGTGAGTG CCAATGGCCACCAGAAGCAAGTAGAAAAGAGCCAGAACAAAGCCAAGCAGTGCAAGGAGTCAGCCACAGA GGCAGAAAGAGTGTACAGGCAAAATATCGAACAACTGGAGAGAGCGAGGACCGAGTGGGAGCAGGAGCAC CGGACTACCTGTGAGGCCTTCCAGTTGCAGGAGTTTGACCGGCTCACCATCCTCCGCAACGCCCTGTGGG TGCACTGTAACCAGCTCTCCATGCAGTGTGTCAAGGATGATGAGCTCTATGAGGAAGTGCGGCTGACCCT TGAGGGCTGTGATGTGGAAGGTGACATCAATGGCTTCATCCAGTCCAAGAGCACTGGCAGAGAGCCCCCA GCTCCGGTGCCTTATCAGAACTACTATGACAGGGAGGTGACCCCACTGATTGGCAGCCCTAGCGTCCAGC CCTCCTGTGGTGTGATAAAGAGGTTCTCTGGGCTGCTACATGGAAGTCCCAAGACCACACCTTCTGCTCC TGCTGCTTCCACAGAGACTCTGACTCCCACCCCTGAGCGGAATGAGTTGGTCTACGCATCCATCGAAGTG CAGGCGACCCAGGGAAACCTTAACTCATCAGCCCAGGACTACCGGGCACTCTACGACTACACCGCACAGA ATTCTGATGAGCTGGACATTTCCGCGGGAGACATCCTGGCGGTCATCCTGGAAGGGGAGGATGGCTGGTG GACTGTGGAGCGGAACGGACAACGTGGCTTTGTCCCTGGGTCGTACTTGGAGAAGCTCTGAGGAAAGGCT AGCAGTCTCCACATACCTCCGCCCTGACTGTGAGGTCAGGACTGTTTCTTTCCATCACCGCCCAGGCCTC ACGGGGCCAGAACCAAGCCCGGTGGTGCTGGGAATGGGCTGGGTGCTGGCTACTCTCAATAAATGTCTCC CAGAAGGAGTAAAGCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30040955 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30040955 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11414 same embryo
	EMAGE:11418 same embryo
	EMAGE:11417 same embryo
	EMAGE:11416 same embryo
	EurExpress:euxassay_006607 same experiment
	MGI:4827497 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source