EMAGE mouse gene expression spatial database

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EMAGE:11418

Xlr3a X-linked lymphocyte-regulated 3A ( MGI:109506)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006613. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006613_01	euxassay_006613_02	euxassay_006613_03	euxassay_006613_04

euxassay_006613_05	euxassay_006613_06	euxassay_006613_07	euxassay_006613_08	euxassay_006613_09

euxassay_006613_10	euxassay_006613_11	euxassay_006613_12	euxassay_006613_13	euxassay_006613_14

euxassay_006613_15	euxassay_006613_16	euxassay_006613_17	euxassay_006613_18	euxassay_006613_19

euxassay_006613_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11418_wholemount_strong.wlz

11418_wholemount_moderate.wlz

11418_wholemount_weak.wlz

11418_wholemount_possible.wlz

11418_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11418_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 13 14 15

pancreas

weak

regional

weak expression: see section 08 09 10

diencephalon

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

vibrissa

weak

regional

weak expression: see section 04 05 17 18 19

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

hindbrain

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

midbrain

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 04 weak expression: see section 05 06 13 14

trigeminal v ganglion

moderate

single cell

moderate expression: see section 02 03 04 16 17 weak expression: see section 05 06 13 14 15

vestibulocochlear viii ganglion

weak

single cell

weak expression: see section 06

spinal cord mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13

dorsal root ganglion

moderate

single cell

moderate expression: see section 06 08 09 10 11 13 14

neural retina

weak

regional

weak expression: see section 01 02 03 18 19 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 13 14 15

ovary

moderate

regional

moderate expression: see section 04 05 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6673
Entity Detected:	Xlr3a, X-linked lymphocyte-regulated 3A ( MGI:109506)
Sequence:	sense strand is shown >T6673 CGACCAAGTGGGATGAACCTCTGTGTGGAAGGAGGCTGCCTTGTGGAGACGAGGGGACTAGGACTCCTAT CTAACACAAGAAGATTCTAGCTATGTCAAGCAGGGAAAGGAAGGCCACTGACACTGCTGGCAGGCACTCA AGGATGGATCCAAATCTCTCCTCAGATGACAGCCAGAACCCAGCTAACAGAGAAGTCCTTGATGCTGGTA GGGAGGACATTATTTCCTCAGGAACAGAGAGGCAACAGGCCAGGAAGGAAAAACAGGACTTGGTCCAAGA ATTCGAAGAGCCACCTAACAAAGTTCTTCAGGAAAACAGAGAAAAATTCTCAAGGATCATGACCTCATCT TTCAGTGCCATGGAGGTCAAAATTAAGGATGTTCTGAAAACCCAGTGTGAACAGAGGCAGAAACTTTGTC AGGACTATTCTCTTCAGTTTACAAATTTGAGTAGGAAGTTAACTTCGGATGCATACAAACTCAAGAAACA GGCCGAAACACTCTCTAATATGTTTATGGAGCAACAGAAGTTTATTCATGAAACTCTCACTCTCCAGAAG AACAGAATGGAGGAATTTAAATCACTGTGTGAAAAATACTTGGAGAAACTGGAGGTACTGAGGGATTCTC GGGGAAATTCCATCGCTGAAGAGCTGAGACGTCTCATAGCCACCTTGGAAATCAAACTTCTGATGCTGCA TAACCAGCAAAACACTGCTGCTCCTCCACAGTCTCTCCTGGATGTGTTATTCTCATAAAACTTTGAAGCA CAAAAGCCGATATGAGGGGAGCAGTATAATCATCTGGGTGAAGCTGCTAGTGCTGATGATTGCAGCTTCG GTGCCTGGATCTTCTCCTGTCTTCCTCCTGTTCCTGAAGATGCCCCGTTCTGTTAAAATCCAAATAAACC CTGGGATTTGTGTGACAGCAGTGCAAATATGTCTGTAGGGAACTCCAGCCCTCGGGTAGAGATGGCAGGC TTTCCTTGGTAGCATCGCAATTACTGGTTAGCACACATTGGTGTTGATACAGTAGATTATCATACCTGAA CATCATCTCCCCAAATTGGAAGGCACACAAGTGGGCTATTGTTGTCGTGTCCAGATAAATAATTAGAGAA GATACAGGCTTTCTTTGACATTCTGCTCTGAAATGTGTTCAGTGATGGAGCCTATGAAACTGACCTTCAG CCTCCAAAGGCAGTGTGCTCTTAATAGTTAAAGTAGTAGAATTCATCAAACCTCCCGAGTAAGAAAGATG TGAGTAACGTGGCTTGAATCAGTGGAAATGCCATTTGCAGACAACATTTACAAGGTCTTTCAATTAAATG AATCTGTCACACTAAGAGGTGATTCTGAACATGAATACAGCTTAATTCGCCATCATTTATTAATCCCTCA CTTGTATGGATAGGAGTAACTTAGAATGTTTTGTTTGTGGAATTTATCGGCCATGTAGTATGGGATCATC AGGGATATTATATATGAGGTCTCGTGTACAGTTAAAGCTCGCGTCCTGTGATTTCCTGTATGGATACCCT GGGTAATCCTTACTTGGGAACGTTTGATTTAATAAACATGAAATAGTTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601144 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601144 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11415 same embryo
	EMAGE:11414 same embryo
	EMAGE:11417 same embryo
	EMAGE:11416 same embryo
	EurExpress:euxassay_006613 same experiment
	MGI:4829242 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source