EMAGE mouse gene expression spatial database

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EMAGE:11422

Tra2a transformer 2 alpha homolog (Drosophila) ( MGI:1933972)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006637. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006637_01	euxassay_006637_02	euxassay_006637_03	euxassay_006637_04

euxassay_006637_05	euxassay_006637_06	euxassay_006637_07	euxassay_006637_08	euxassay_006637_09

euxassay_006637_10	euxassay_006637_11	euxassay_006637_12	euxassay_006637_13	euxassay_006637_14

euxassay_006637_15	euxassay_006637_16	euxassay_006637_17	euxassay_006637_18	euxassay_006637_19

euxassay_006637_20	euxassay_006637_21	euxassay_006637_22	euxassay_006637_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11422_wholemount_strong.wlz

11422_wholemount_moderate.wlz

11422_wholemount_weak.wlz

11422_wholemount_possible.wlz

11422_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11422_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7105
Entity Detected:	Tra2a, transformer 2 alpha homolog (Drosophila) ( MGI:1933972)
Sequence:	sense strand is shown >T7105 CTGGTGGGAGTGGAACGCACTGGCCGTTGTAGCCGTCGCCTTCTTCCTCCCCACAGTCCGAACAGCGCGG TGACTAGGCCGTCGGCCCTCGAGCCGAGACATATCTCTTATTTAGTTCTCGGGAGCGCCTGGCCGACATG AGTGATGTAGAGGAGAACAACTTCGAGGGCAGAGAGTCTCGCTCTCAGTCAAAATCTCCAACGGGAACTC CTGCTCGTGTAAAATCAGAGAGCAGGTCAGGATCTCGTAGTCCATCACGGGTGTCCAAACATTCTGAATC CCATTCTCGATCAAGATCAAAGTCCAGATCAAGGTCAAGGAGGCATTCGCATAGACGCTACACTCGATCC AGGTCACACTCTCATAGGAGACGATCTCGGAGTAGGTCCTATACACCCGAGTATAGGCGGCGGAGGAGCC GGAGTCACTCTCCAATGTCTAATCGCAGAAGGCATACTGGAAGCAGGGCAAACCCAGATCCCAACACTTG CTTGGGAGTATTTGGCCTCAGTTTGTACACAACAGAGAGAGATCTTCGTGAAGTGTTTTCTCGATATGGA CCTCTGAGTGGTGTTAATGTAGTTTATGATCAGCGAACTGGGAGGTCACGTGGATTTGCTTTTGTGTATT TTGAGAGAATAGATGACTCTAAGGAGGCTATGGAGAGAGCAAATGGGATGGAGCTGGACGGTAGAAGAAT CCGTGTGGATTATTCTATCACCAAGAGAGCGCACACACCCACACCAGGCATTTACATGGGCAGACCAACT CACAGCGGTGGTGGAGGTGGCGGAGGAGGAGGAGGTGGCGGAGGAGGAGGTGGTGGCGGTGGTAGACGAC GAGATTCTTACTACGATAGAGGATATGATCGAGGCTATGACAGATACGAAGATTATGATTACCGAAGAAG GTCACCCTCTCCTTACTACAGTCGCTACAGGTCACGATCGAGATCCCGTTCCTATAGTCCAAGACGCTAT TGATACTGGAGTGATTGTGCCCGAGGACTTTGCTTTTTCTTCTTTTTGATTTTGGATTCCCAAGCTTCCA TGCTTCCTAGTCCTTTAAACCAAAACCAAACAACCTGGTTTATTTAATCTCTGCGTGACCAGTTGTGTTG CTGTTTCCACCCTTTGTTATACTCTAAAGGTGTAATTGTCTTTTCAGTAGTGAACATCCTGAGTGAAAGG GAGCCCGGGTTCTGCTTTGTGGTTCTTCTTTGCCTTTACAGAGGATTAACTTCTTATTTGAGGACAGAAA CCATCTTTTGAAACTTTTTCTGATGGATATTGTGGTAGACATTAGAAATCTGTGGTATGATAAACTGCTT TTTAATCTTCTTGGGGAAGGTAAATAACACTTTAAGTAGGTCAGTCATGCCAGCTTTGTCTGGAGTGGCA TGGAGCAGTTGAATAGTTGAGTGTAGAATGTTTGAAGCTCTAGGAGAAAATGTGGGACATTTCTTTTGAA GAATATAACTTTGAACCTAAGAATTAAGTCACCTTTTAGAAATTAAAAGCTTGTAATTCCTATAAGACAT GTTGTATTTGAATTTACATGTTAAACCTTATAAACTTAAAGTGTGATTTTTATGTTAAATTTACAGAAAC TTAAGTATTCCCTTAATCACTGAAGGACAACCTTTATTTATTAACTAGAACAATTGTATAACTTGCTGTT AGAAATTTTGGTATTGGGACATTGGTTACATGGGTTATTGTATAGGATCCTAAAATACATGTGGGGAAGA GTAATTTCAAATAAAAGTTAATTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4016620 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4016620 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11424 same embryo
	EMAGE:11426 same embryo
	EMAGE:11425 same embryo
	EMAGE:11427 same embryo
	EMAGE:11423 same embryo
	EurExpress:euxassay_006637 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source