EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11426

Riok3 RIO kinase 3 (yeast) ( MGI:1914128)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006646. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006646_01	euxassay_006646_02	euxassay_006646_03	euxassay_006646_04

euxassay_006646_05	euxassay_006646_06	euxassay_006646_07	euxassay_006646_08	euxassay_006646_09

euxassay_006646_10	euxassay_006646_11	euxassay_006646_12	euxassay_006646_13	euxassay_006646_14

euxassay_006646_15	euxassay_006646_16	euxassay_006646_17	euxassay_006646_18	euxassay_006646_19

euxassay_006646_20	euxassay_006646_21	euxassay_006646_22	euxassay_006646_23	euxassay_006646_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11426_wholemount_strong.wlz

11426_wholemount_moderate.wlz

11426_wholemount_weak.wlz

11426_wholemount_possible.wlz

11426_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11426_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 07 08 09 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19 20

trigeminal v nerve

moderate

regional

moderate expression: see section 11 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 18

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 16 17 18 19

not examined

regional

not examined expression: see section 01 02 03 04

neural retina

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 05 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7102
Entity Detected:	Riok3, RIO kinase 3 (yeast) ( MGI:1914128)
Sequence:	sense strand is shown >T7102 CGGCAGCGGGTTGCCCGTGCCCCGCCCGTGTCCTCGGCGGAGCCCTTTCTTCCTCTTGTCACCTCTCGAC TCCGTTCTCGTCTCTGCCTTCTTCCCGGAATGGATCTGGTCGGAGTGTCTTCGCCTGAGCCAGGGCCCGC AGCAGCTTGGGGACCCAGTAAGTGCCCATGGGCTACCCCTCAAAACACAGTATCCTGTTCACTGACCGAG GTGATGAGTGAGGAGCTGGCTAAAGAGCTGCAGTTGGAAGAAGAAGCGGCTGCTTTCCCTGAAGTTGTTG TTGCTGAAGGACCATTCATTTCTGGAGAAAACATTGACACTTCCAGTGACCTAATGCTGGCTCAGATGCT ACAGATGGAATTTGACAGAGAATATGATGCACAGCTTAGGCGTGAGGAAAAGAAGTTCAATGGTGATAGC AAAGTTTCCATTTCGTTTGAAAACTATCGCAAAGTTCATCCTTTTGAAGACAGTGACAGCTCTGAAGACG AGGTTGACTGGCAGGACACTCGAGACGATCCCTACAGACCAGCAAAGCCAATTCCTACTCCCAAAAAGGG TTTTATTGGAAAAGGAAAAGACATCACCACCAAGCATGATGAAGTAGTATGTGGAAGAAAGAACACAGCA AGAATGGAAAATTTTGCCCCTGGCTTTCAGGTAGGGGATGGAATTGGAATGGATTTGAAACTATCCAACC ACGTTTTTAATGCCTTAAAACAACACGCCTACTCAGAAGAGCGTCGGAGCGCCCGTCTCCACGAGAAGAA GGAACATTCTACCGCTGAAAAAGCAGTTGATCCTAAGACACGCTTACTTATGTATAAAATGGTCAACTCT GGAATGTTGGAGACAATCACTGGCTGTATTAGTACAGGAAAGGAATCTGTTGTCTTTCATGCATATGGAG GGAGCCTGGAGGATGAAAAGGAAGATGGTAAGGCTATACCAACAGAATGTGCCATCAAGGTATTTAAAAC AACCCTTAATGAGTTTAAGAATCGTGACAAGTACATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGT AAACTAAATCCACGTAAGATCATCCGCATGTGGGCAGAGAAAGAAATGCACAATCTTACAAGAATGCAGA AGGCTGGAATTCCTTGTCCAACAGTTGTACTGCTTAAGAAGCACATTTTAGTTATGTCTTTCATTGGCCA TGATCAAGTTCCAGCCCCTAAATTAAAAGAAGTAAAGCTTAGTAATGAAGAAATGAAAGATGCCTACTAT CAGACTCTTCATTTGATGCAGCAATTATATAACGAATGTACCCTTGTGCATGCTGACCTCAGCGAGTATA ACATGTTGTGGCATGCTGGGAAGGTCTGGTTGATTGACGTCAGTCAGTCGGTAGAGCCAACCCATCCTCA TGGCCTGGAGTTCTTATTCCGTGACTGTAGGAATGTTTCACAGTTCTTCCAGAAAGGAGGAGTAACGGAA GCCCTCAATGAACGGGAGCTGTTCAATGCTGTTTCTGGCCTGAACATCTCGGCAGACAATGAAGCTGATT TCTTAGCTGAGATAGAAGCTTTGGAGAAGATGAATGAAGATCACGTTCAGAAGAACGGGAGGAAAGCAGC CTCGTTTCTGAAAGATGATGGAAGCCCACCTGTGCTGTCTGCCGACTAGTACAGCGGTGACGGACGGCAG CTGCCATAGCCAGCGAGTGGAGGTGACCTGACATACCAAAGCAGAAGTGGACAGTGCTGCTTCTGGGCTT CTCACTTGTGACCCACGTGCCAGATGTGTGCAATTTTTAACTCGGCATTGAAAGAATAAAATGTCACTAC CTCTCATCTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4016197 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4016197 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11424 same embryo
	EMAGE:11422 same embryo
	EMAGE:11425 same embryo
	EMAGE:11427 same embryo
	EMAGE:11423 same embryo
	EurExpress:euxassay_006646 same experiment
	MGI:4827746 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source