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EMAGE:11427

Med28 mediator of RNA polymerase II transcription, subunit 28 homolog (yeast) ( MGI:1914249)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427
"Pseudo-wholemount" of euxassay_006719. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006719_01 euxassay_006719_02 euxassay_006719_03 euxassay_006719_04
EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427
euxassay_006719_05 euxassay_006719_06 euxassay_006719_07 euxassay_006719_08 euxassay_006719_09
EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427
euxassay_006719_10 euxassay_006719_11 euxassay_006719_12 euxassay_006719_13 euxassay_006719_14
EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427
euxassay_006719_15 euxassay_006719_16 euxassay_006719_17 euxassay_006719_18 euxassay_006719_19
EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427 EMAGE:11427
euxassay_006719_20 euxassay_006719_21 euxassay_006719_22 euxassay_006719_23 euxassay_006719_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11427Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11427_wholemount_strong.wlz
11427_wholemount_moderate.wlz
11427_wholemount_weak.wlz
11427_wholemount_possible.wlz
11427_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11427_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7110
Entity Detected:Med28, mediator of RNA polymerase II transcription, subunit 28 homolog (yeast) ( MGI:1914249)
Sequence:sense strand is shown

>T7110
CGGACGCGTCCGCCCACGCGTCCGGCCCGCCGTTGCAGACATGGCGGCGTCCCTGGGAGGGATGTTCACT
GGGCAGCCTCCAGGTCCTCCGCCGCCGCCGCCCGGGCTCCCAGGCCAGGCGTCGCTTCTGCAAGCAGCCC
CTGGGGCCCCGAGACCATCTAACAGCACTTTGGTGGACGAGTTGGAGTCATCCTTCGAGGCTTGCTTTGC
TTCCCTTGTGAGTCAGGATTATGTCAATGGGACTGATCAGGAAGAAATTCGAACTGGTGTTGATCAGTGT
ATCCAGAAGTTTTTGGACATTGCAAGACAGACAGAATGTTTTTTCCTACAAAAAAGGTTGCAGTTATCTG
TCCAGAAACCAGATCAAGTTATCAAAGAGGATGTCTCGGAACTAAGGAGTGAACTGCAGCGGAAGGATGC
GCTGGTCCAGAAGCACTTGACAAAGCTGAGGCATTGGCAGCAGGTGCTGGAGGACATCAATGTGCAGCAC
AAGAAGCCGGCTGACATGCCTCAGGGATCCTTGGCCTTCCTCGAGCAGGCGTCTGCCAACATTCCTGCAC
CTCTGAAGCAGACCTAAGCACTCCTGCCTGTCTTGCAGCCTTGCAGTCTTGCCAGACACGTTTCCTTACA
GACCTGGTGTGTTGGAAGGACTCTGTTGGATAATGAGTTTGTATCAGTTTCTGTCCCTATTTATAAGGTA
GGGTTTCATTATGAAGCCGGGAAGGGCCTTGAATCTATGATCCCCTGCCTCAGCTCCTCTAGTGCTCTAA
ACTCCTGAATACTTTATAAAAAACCTCTGTCTTTGATAAAGTAAAGTTTGGCTTTGTTTGTTAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4017146 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017146 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11424 same embryo
 EMAGE:11422 same embryo
 EMAGE:11426 same embryo
 EMAGE:11425 same embryo
 EMAGE:11423 same embryo
 EurExpress:euxassay_006719 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS