EMAGE mouse gene expression spatial database

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EMAGE:11434

Gata1 GATA binding protein 1 ( MGI:95661)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006598. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006598_01	euxassay_006598_02	euxassay_006598_03	euxassay_006598_04

euxassay_006598_05	euxassay_006598_06	euxassay_006598_07	euxassay_006598_08	euxassay_006598_09

euxassay_006598_10	euxassay_006598_11	euxassay_006598_12	euxassay_006598_13	euxassay_006598_14

euxassay_006598_15	euxassay_006598_16	euxassay_006598_17	euxassay_006598_18	euxassay_006598_19

euxassay_006598_20	euxassay_006598_21	euxassay_006598_22	euxassay_006598_23	euxassay_006598_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11434_wholemount_strong.wlz

11434_wholemount_moderate.wlz

11434_wholemount_weak.wlz

11434_wholemount_possible.wlz

11434_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11434_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

spotted

moderate expression: see section 12 13 14 15 16 17

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8084
Entity Detected:	Gata1, GATA binding protein 1 ( MGI:95661)
Sequence:	sense strand is shown >T8084 AAGAGGTAATCCGGGAGACTCAGAGGCCAAGGCCAGTGAGGACTCCCTTGGGATCACCCTGAACTCGTCA TACCACTAAGGTGGCTGAATCCTCTGCATCAACAAGCCCAGGTTCAACCCCAGTGTTCCCATGGATTTTC CTGGTCTAGGGGCCCTGGGGACCTCAGAACCCTTGCCCCAGTTTGTGGATTCTGCCCTGGTGTCCTCACC ATCAGGTTCCACAGGTTTCTTTTCCTCTGGGCCAGAGGGTTTGGATGCAGCATCTTCTTCCACTTCCCCA AATGCAGCCACGGCCGCAGCATCAGCACTGGCCTACTACAGAGAAGCTGAGGCCTACAGACACTCCCCAG TCTTTCAGGTGTATCCACTGCTCAACAGTATGGAGGGAATTCCTGGGGGCTCACCTTATGCTAGCTGGGC CTATGGCAAGACGGCACTCTACCCTGCCTCAACTGTGTGCCCCAGCCATGAGGATGCCCCTTCCCAGGCC CTGGAGGACCAGGAAGGGAAGAGCAGCAACACGTTCTTGGACACCTTGAAGACGGAGCGGCTGAGTCCAG ACCTCCTGACGCTGGGGACTGCACTGCCTGCATCGCTCCCTGTCACCGGCAGTGCTTACGGGGGAGCTGA CTTTCCCAGTCCTTTCTTCTCTCCCACTGGGAGCCCTCTCAGCTCAGCAGCCTACTCTTCCCCCAAGTTT CATGGAAGCCTGCCATTGGCCCCTTGTGAGGCCAGAGAGTGTGTGAACTGTGGAGCAACGGCTACTCCAC TGTGGCGGAGGGACAGGACAGGTCACTACCTGTGCAATGCCTGTGGCTTGTATCACAAGATGAATGGTCA GAACCGGCCTCTCATCCGGCCCAAGAAGCGAATGATTGTCAGCAAACGGGCAGGCACCCAATGCACTAAC TGTCAAACGACCACTACAACACTCTGGCGGAGGAATGCCAGCGGAGATCCGGTATGCAATGCCTGCGGCC TCTATTTCAAGCTCCATCAGGTGAACCGCCCACTGACCATGAGGAAAGATGGAATCCAGACGAGGAACCG CAAGGCATCTGGCAAAGGGAAAAAGAAGCGGGGGTCGAATCTGGCTGGAGCGGGAGCGGCTGAAGGACCA GCTGGTGGCTTCATGGTGGTAGCTGGTAGCAGCAGTAGTGGGAATTGTGGGGAGGTGGCCTCAGGCTTGG CACTGGGCACTGCAGGTACTGCCCACCTCTATCAGGGCCTAGGACCTGTGGTACTGTCAGGGCCTGTCAG CCATCTTATGCCTTTTCCTGGACCTCTGCTGGGATCGCCTACAACCTCCTTCCCCACCGGTCCTGCACCT ACCACCAGCAGCACCAGCGTGATCGCCCCACTCAGTTCTTGAAGGTACACAGAATAGCCTTGACCTTGTG GCAGAGGAGACCCCCCCTTTTTTTGGAGACAGGATCTTCTGTAGCTATGTAGCTATGAACTATGTAGATA AGGGTGACCCCACATTTCCCATTCTCCTGCCTCTACCTCCCGAGTACTGAGATTCAGGCATGTATTGCTA TGCCTGACATTTTCCTTCCTTTCAGTTTTGTTTTGTGGGGTTTGTTGTTGTTGTTTTGAGACAGGGTTTC ACTATGCAGCCTAGGCTGGCTTTGAATTCCTTATGTAGTCCAGACTAAGCTCCAGCTTGAGATCCTTGTG CCTCAGTTCCTGAGTGCTGGGATTACAGGCATGTGCTACCGTGCCCGTTTCTCCCTCCTCTTAGAGCCAC TGGCCTTTACAGCCCAACCATCTCCTTGCCCTGCACCCTTAAAGATTTTGTAAAATAAAACCACCTGGAG ACATGCGCTGGTATTTGGTTAAATAAATGTCAGTGTCAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:30039400 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30039400 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11431 same embryo
	EMAGE:11432 same embryo
	EMAGE:11433 same embryo
	EMAGE:11430 same embryo
	EMAGE:11429 same embryo
	EurExpress:euxassay_006598 same experiment
	MGI:4825002 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source