EMAGE mouse gene expression spatial database

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EMAGE:11436

Clec12a C-type lectin domain family 12, member a ( MGI:3040968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006597. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006597_01	euxassay_006597_02	euxassay_006597_03	euxassay_006597_04

euxassay_006597_05	euxassay_006597_06	euxassay_006597_07	euxassay_006597_08	euxassay_006597_09

euxassay_006597_10	euxassay_006597_11	euxassay_006597_12	euxassay_006597_13	euxassay_006597_14

euxassay_006597_15	euxassay_006597_16	euxassay_006597_17	euxassay_006597_18	euxassay_006597_19

euxassay_006597_20	euxassay_006597_21	euxassay_006597_22	euxassay_006597_23	euxassay_006597_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11436_wholemount_strong.wlz

11436_wholemount_moderate.wlz

11436_wholemount_weak.wlz

11436_wholemount_possible.wlz

11436_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11436_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 11 13 14 15 weak expression: see section 12

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18 19

liver left lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

liver right lobe

strong

spotted

strong expression: see section 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8155
Entity Detected:	Clec12a, C-type lectin domain family 12, member a ( MGI:3040968)
Sequence:	sense strand is shown >T8155 GTCTGGCATAGTGGTTTTTCAAAAGGAAATTTGAGCTTGTGAGGTTAGCCACAGTTTACAGAGGGTTGAA AGTCTAGGTAGGCTTACTTAACTGTCAGCCCTCTCACCTTTTTCGAAGGACATTTTCTGGAGCCTTCTTT GAATATTCATCAATGTCTGAAGAAATTGTTTATGCAAATCTCAAAATCCAGGACCCTGATAAAAAAGAAG AAACCCAGAAGTCTGACAAATGTGGGGGAAAAGTATCCGCCGATGCTTCCCATTCACAGCAAAAAACAGT CTTGATTCTGATTCTTCTATGCCTTCTGCTGTTCATTGGAATGGGGGTCTTAGGAGGCATCTTTTATACA ACTTTGGCAACAGAAATGATAAAATCGAATCAATTGCAAAGGGCCAAGGAAGAACTTCAGGAAAATGTTT TCCTACAGCTGAAGCACAATCTCAACAGCTCCAAGAAAATCAAGAACCTTTCTGCCATGCTGCAAAGCAC AGCCACCCAGCTGTGCCGAGAGCTGTATAGCAAAGAACCAGAGCACAAATGTAAACCATGTCCAAAGGGT TCAGAATGGTACAAGGACAGCTGTTATTCTCAACTCAATCAGTATGGAACATGGCAAGAGAGTGTCATGG CCTGCTCTGCTCGGAATGCCAGCCTCCTGAAGGTTAAGAACAAGGATGTGCTGGAATTTATAAAGTACAA GAAGCTACGCTATTTTTGGCTTGCATTGTTGCCCAGAAAAGATCGCACACAATATCCACTAAGTGAGAAG ATGTTCCTCTCTGAAGAGTCTGAAAGAAGCACAGATGACATAGATAAGAAGTACTGCGGATATATAGACA GGGTCAATGTTTATTATACATACTGCACTGATGAGAACAATATCATATGTGAAGAGACAGCCAGCAAGGT GCAGTTGGAAAGTGTGTTGAATGGCCTCCCAGAGGATAGCAGGTAGCTACTCTGAGTTGCTTGTCCCTAG CTGCTCTCCTGCTGTGATGGTTTGAATGAGAATGGCCCCCAAGGGCTCATTTGAATACTTGGTCCTCTGC TGGTGGAACTGTTTCAGAGACACCAGAAGGTGTGACCTTGTTAGAGGAGGTTGTGTCATTGAGAGTGGGC TTTGCGTTTTCAAAAGCCCATGCCATTCCCAGTTAGTTCTCTATCTTGTGCTTGTGGATTGAGCTGTGAG CCCACAGCTACTGCTTAGGACCTTGTCTGCCTGCATGCTGCTATCCTCCTCACCACAATGGTCATGGACT CTAACCCTCCCAAACCAGGAGCCAAATTAAATGCTTCTTTTTATAAGTAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30044572 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30044572 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11439 same embryo
	EMAGE:11437 same embryo
	EMAGE:11435 same embryo
	EMAGE:11438 same embryo
	EurExpress:euxassay_006597 same experiment
	MGI:4823918 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source