EMAGE mouse gene expression spatial database

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EMAGE:11463

Pnlip pancreatic lipase ( MGI:97722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006948. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006948_01	euxassay_006948_02	euxassay_006948_03	euxassay_006948_04

euxassay_006948_05	euxassay_006948_06	euxassay_006948_07	euxassay_006948_08	euxassay_006948_09

euxassay_006948_10	euxassay_006948_11	euxassay_006948_12	euxassay_006948_13	euxassay_006948_14

euxassay_006948_15	euxassay_006948_16	euxassay_006948_17	euxassay_006948_18	euxassay_006948_19

euxassay_006948_20	euxassay_006948_21	euxassay_006948_22	euxassay_006948_23	euxassay_006948_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11463_wholemount_strong.wlz

11463_wholemount_moderate.wlz

11463_wholemount_weak.wlz

11463_wholemount_possible.wlz

11463_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11463_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pons mantle layer

strong

regional

strong expression: see section 07 18 moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9940
Entity Detected:	Pnlip, pancreatic lipase ( MGI:97722)
Sequence:	sense strand is shown >T9940 GTCCGTAGAACCTGACGGATCCGCCACGATGCTAATGCTGTGGACATTTGCAGTGCTGCTGGGAGCAGTA GCTGGAAGGGAAGTTTGCTTCGACAAACTCGGCTGCTTCAGTGATGATGCCCCCTGGTCAGGAACTCTAG ACAGACCCCTGAAAGCACTGCCCTGGAGCCCAGCACAGATCAACACCCGCTTTCTCCTCTACACCAACGA GAATCCAGACAACTACCAGCTAATTACTTCAGATGCATCAAACATCAGAAACTCCAATTTCCGAACAAAC AGAAAAACCCGTATCATTATCCACGGCTTCATCGACAAGGGAGAAGAAAACTGGCTATCTGACATGTGCA AGAACATGTTCCGAGTGGAGAGTGTGAACTGCATCTGTGTGGACTGGAAAGGCGGTTCCCGAACGACATA TACCCAGGCTACCCAGAACGTCCGGGTCGTGGGGGCAGAAGTGGCATTGTTGGTTAACGTTCTGCAGTCT GATCTGGGATACTCACTTAACAATGTCCACCTGATTGGCCACAGCCTGGGTTCCCACATTGCTGGGGAGG CTGGAAAGAGGACCTTTGGAGCCATTGGAAGGATCACAGGGTTGGACCCCGCTGAGCCTTACTTCCAGGG CACCCCTGAAGAAGTCCGACTGGACCCCACCGATGCTCAGTTTGTGGACGCAATTCACACAGACGCCGGG CCCATTATCCCCAACTTAGGATTTGGAATGAGCCAGACTGTGGGTCACTTAGATTTCTTTCCAAACGGAG GAATCGAAATGCCAGGATGCCAGAAGAATATTCTTTCCCAAATTGTTGACATCGACGGGATCTGGGAAGG AACTCGAAACTTTGCTGCCTGTAACCACTTAAGAAGCTACAAGTTTTACACCGACAGCATCGTCAACCCA ACTGGCTTTGCTGGGTTCTCCTGTAGCTCTTACAGTCTTTTCACGGCAAATAAGTGCTTCCCATGTGGGA GTGGAGGATGCCCCCAGATGGGTCATTACGCTGACAGATATCCTGGGAAAACAAGCAGACTCTACCAGAC ATTTTACCTCAACACCGGTGATAAGAGCAACTTCGCACGTTGGCGGTACCAGGTGACTGTCACCCTGTCT GGACAGAAGGTCACTGGGCACATTCTAGTTTCTTTGTTTGGAAATGGAGGAAACTCTAAACAGTATGAAG TTTTCAAGGGCTCTCTGCAGCCAGGTACTTCTCACGTCAATGAATTCGACTCTGATGTGGATGTTGGAGA TTTGCAGAAGGTTAAATTTATTTGGTACAACAATGTGATCAACCCAACTCTACCCAAAGTGGGAGCATCA AGGATCACAGTGGAAAGAAATGATGGCAGAGTGTTCAACTTCTGTAGTCAAGAGACAGTGAGGGAAGACG TCCTGCTCACACTGTCTCCATGTTAGGAGGCTGCTGCTGTGTGACCACCAAGTCCCACTGTTGTAATAAA GTCTAGTATTAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30316303 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30316303 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827297 same experiment
	EurExpress:euxassay_006948 same experiment
	EMAGE:29741 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source