EMAGE mouse gene expression spatial database

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EMAGE:11465

Abhd14b abhydrolase domain containing 14b ( MGI:1923741)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006897. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006897_01	euxassay_006897_02	euxassay_006897_03	euxassay_006897_04

euxassay_006897_05	euxassay_006897_06	euxassay_006897_07	euxassay_006897_08	euxassay_006897_09

euxassay_006897_10	euxassay_006897_11	euxassay_006897_12	euxassay_006897_13	euxassay_006897_14

euxassay_006897_15	euxassay_006897_16	euxassay_006897_17	euxassay_006897_18	euxassay_006897_19

euxassay_006897_20	euxassay_006897_21	euxassay_006897_22	euxassay_006897_23	euxassay_006897_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11465_wholemount_strong.wlz

11465_wholemount_moderate.wlz

11465_wholemount_weak.wlz

11465_wholemount_possible.wlz

11465_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11465_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24 weak expression: see section 13 14 15

anterior naris

moderate

regional

moderate expression: see section 15 18

external naris

moderate

regional

moderate expression: see section 14 weak expression: see section 19

posterior naris

moderate

regional

moderate expression: see section 14 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 17 18

kidney calyx

moderate

regional

moderate expression: see section 08 21 weak expression: see section 09 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7355
Entity Detected:	Abhd14b, abhydrolase domain containing 14b ( MGI:1923741)
Sequence:	sense strand is shown >T7355 CGTCAATAGCACACGCCATTCTCCTGCGGAAGACCACGCACATTTCCAGGCACATCACCACTGGGCATGG CCGGTGTGGACCAGCATGAGGGCACCATCCAGGTGCAGGGCCAGAACCTCTTCTTCAGAGAGACCCGGCC TGGGAGTGGGCAGCCTGTCCGCTTCTCTGTGCTGCTGCTGCATGGTATCCGCTTCTCCTCGGAGACCTGG CAGAACCTGGGTACTCTGCAAAGGCTGGCTGAGGCTGGCTACAGGGCTGTGGCCATTGATCTGCCAGGTC TTGGGCGATCCAAGGAAGCAGCAGCCCCTGCCCCTATTGGGGAACCGGCCCCTGGCAGCTTCCTAGCTGC TGTGGTGGATACCTTGGAGCTGGGACCCCCAGTTGTGATCAGTCCATCATTAAGTGGCATGTATTCACTG CCCTTCCTCGTGGCCCCCGGGTCCCAACTCCGGGGCTTTGTACCAGTGGCTCCTATCTGCACAGACAAAA TCAATGCTGTGGACTACGCCAGTGTGAAGACTCCAGCTCTCATTGTATATGGAGACCAGGACCCCATGGG CTCCTCCAGCTTTCAACACCTGAAGCAGCTGCCCAACCACAGAGTGCTGGTCATGGAAGGGGCAGGGCAT CCCTGTTACTTGGACAAGCCTGATGAGTGGCATAAGGGGCTGCTGGATTTCCTGCAAGGGCTAGCCTGAG ACTCAGTCCTGATGATAGGAGGTTACAGACTGCCTGCTCTAGGATCTCTTGCTGTCTTCTCCTGGTCTCT GGCTTCTGCCTCACCTGCAACAGGTCTGTCTATACATCTGTCTGGGTTGCTGTCATTTCTGTTTTTCTCT TGCAGTGACAGATCAGATCAAGGCGGTAAATCAAGTGGAAAAAGCGTCGGGTTCAAGGGGAGAACTCTGA TTGTGGGTAATCCATAAGCTTCCTCTACAGGCTCCCCGCTTGGTTCTCACTTCTGCTTTACTTCACTTTC TGATCCATCTCTCTTTTCAGCTGCTCCACAGGACAGCCATTCCTTCTGCCGCCCACCCACCCCCCCTTAG ACCACAGACAGCATATGGGTATTGCTTGTTTAGATCCATCCCACACCCAGACAGAACCTTTCCCTTGGGA GCAGCACAGGGGTCCCACATTCTTGTGTAGTTTGCACAGGTACATGTGCAGTTTCCTGAAGGATGGAGGG AATCTTTGTAAACAGAGATCCACTTGCCAGGATAAAAATGTGGCCACCGCTAGATTTCTCTTACCTACCC GTTGCAACTGGTTCCAGACCAGAATGGCCTCCTCAGAAGGGGAGGTGCTCTTCAAATCTCCCTTAGGGGC TGCATACCTCCCCAGGTAATCAAGCATGGATATTCTCAGCCAAGCCCATTTCCTGCCCTGAGGTTTGGGA GTGGGATAAGGAGAGGAGACCGGGAGGTCCCTGAAATAAAGTGATACAATTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191819 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191819 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11467 same embryo
	EMAGE:11464 same embryo
	EMAGE:11466 same embryo
	EurExpress:euxassay_006897 same experiment
	MGI:4822877 same experiment
	EMAGE:31879 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source