EMAGE mouse gene expression spatial database

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EMAGE:11466

Stk25 serine/threonine kinase 25 (yeast) ( MGI:1891699)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006890. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006890_01	euxassay_006890_02	euxassay_006890_03	euxassay_006890_04

euxassay_006890_05	euxassay_006890_06	euxassay_006890_07	euxassay_006890_08	euxassay_006890_09

euxassay_006890_10	euxassay_006890_11	euxassay_006890_12	euxassay_006890_13	euxassay_006890_14

euxassay_006890_15	euxassay_006890_16	euxassay_006890_17	euxassay_006890_18	euxassay_006890_19

euxassay_006890_20	euxassay_006890_21	euxassay_006890_22	euxassay_006890_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11466_wholemount_strong.wlz

11466_wholemount_moderate.wlz

11466_wholemount_weak.wlz

11466_wholemount_possible.wlz

11466_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11466_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 05 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19 20

trigeminal v nerve

moderate

regional

moderate expression: see section 10 18

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 17

cervical ganglion

weak

regional

weak expression: see section 10 17

thoracic ganglion

weak

regional

weak expression: see section 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 05

testis

moderate

regional

moderate expression: see section 05 06 20 21 22 weak expression: see section 07 08 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8073
Entity Detected:	Stk25, serine/threonine kinase 25 (yeast) ( MGI:1891699)
Sequence:	sense strand is shown >T8073 AAGACGTAATCCCTCACGGGCTGGGAGGCCTGGGCGTTCCGGGAGACCGAATTGCGGTGTGAGCCGCCGT GGGGACTGCGAAGCGGGCCCGGAGTCCGAGATGGAGCCGCCCGGAGGTGCTTGAGGCGAGCGGGCGGAGC GGCTGCGACGGACTGGTCACGTCAGCGGGGACATGGCTCACCTCCGGGGCTTCGCGCACCAGCATTCTCG TGTGGACCCAGAGGAACTCTTCACCAAGCTTGACCGCATTGGCAAAGGCTCATTTGGGGAGGTGTACAAG GGGATCGACAACCACACCAAGGAAGTGGTGGCCATCAAGATCATTGACTTGGAAGAGGCCGAGGATGAAA TTGAGGACATCCAACAGGAGATCACTGTGCTCAGCCAATGTGACAGTCCTTATATCACCCGCTACTTCGG CTCCTACCTGAAGAGCACCAAGCTGTGGATTATCATGGAGTACCTGGGTGGTGGCTCTGCACTGGACTTG CTGAAACCTGGTCCCCTGGAAGAGACCTATATTGCCACCATCTTGCGGGAGATCCTGAAAGGCCTGGATT ATCTGCACTCAGAGCGCAAGATCCACCGAGATATCAAAGCTGCCAACGTGCTGCTCTCGGAGCAGGGTGA TGTGAAGCTGGCAGACTTCGGTGTGGCTGGGCAGCTCACAGACACACAAATCAAGAGGAACACATTTGTG GGTACACCCTTCTGGATGGCACCTGAGGTTATCAAGCAGTCGGCCTATGACTTCAAGGCTGACATCCGGT CCCTGGGCATCACAGCCATTGAGCTGGCCAAGGGGGAGCCGCCAAACTCTGATCTCCACCCTATGCGAGT CCTGTTCCTGATTCCCAAGAACAACCCACCCACATTGGAGGGCCACCACAGCAAACCCTTCAAGGAGTTT GTAGAGGCCTGCCTCAATAAGGACCCACGATTCCGACCCACTGCCAAGGAGCTCCTGAAGCACAAATTCA TCACACGATACACCAAGAAGACCTCCTTTCTCACTGAACTTATTGACCGATATAAGCGCTGGAAGTCTGA GGGGCATGGAGAGGAGTCCAGTTCTGAGGATTCTGACATTGATGGCGAGGCTGAGGATGGGGAGCAGGGT CCCATCTGGACGTTCCCTCCAACCATCCGGCCAAGTCCACATAGCAAGCTGCACAAGGGAACTGCTCTGC ACAGCTCACAGAAGCCTGCAGAGCCTATCAAGAGACAGCCAAGGTCCCAGTGCTTGTCCACACTAGTCCG TCCTGTCTTTGGAGAGCTCAAAGAGAAGCACAAGCAGAGCGGTGGGAGCGTGGGTGCCCTGGAGGAGCTA GAGAATGCCTTCAGCCTGGCTGAGGAATCCTGTCCTGGCATCTCAGACAAGCTGATGGTGCACCTGGTGG AGCGTGTGCAGAGGTTCTCACACAGCAGGAACCACCTGACATCTACCCGCTGAACAACCAACTGAATGCA CTACTCTTCACAGAGAGGGAGAAGAATGCTGTTTGTTTTGAGCTGTAAAAGGACTGACTTGGAAGCCCTC TTGTGTTGTGTTACAACTGTAAAGACTGACCAGCCTGTAGGCTTCACAAGCCAGGTTACCTTCTTTCTGC CCTACACAGCAGCCCCTTGACACCTGTGTTCACTCCTTGGTTGTCTGAAGCTATGCTAGGCACCTGGGAG CCCTTGCAGAACTGGGCAGCAACTTGGGAGGCTGGTATGACCTTCTATATAGACTCCTCAGTCCCTGCAG CAGGTAGACCAAGGAGCATAGTAACTATTGGGCAGCTTTGGCTCTTCGTCTACACCAGGGACATTGTCTT GAGGGCTCATAGGGGCCCAAGATGGTCATCATTTGCCTTGTGGTGTCAGTTCAGGTACTGTGTGTTTTCA TGAGTACTCGAATCGTCCAGAATATTTTGGGTTTTTTTTTTTTTTTTTAGGAAAATTGAACTTCCTGAGT ATTCTGACATTAAGTGTTAGTTTTAATAGAGCATTGAAAACCCTACCATTTCAATAAAGACCTGGATTGG AAATGTAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30057101 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30057101 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11467 same embryo
	EMAGE:11464 same embryo
	EMAGE:11465 same embryo
	EurExpress:euxassay_006890 same experiment
	MGI:4828500 same experiment
	EMAGE:31879 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source