EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11476

Slc35a1 solute carrier family 35 (CMP-sialic acid transporter), member 1 ( MGI:1345622)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006887. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006887_01	euxassay_006887_02	euxassay_006887_03	euxassay_006887_04

euxassay_006887_05	euxassay_006887_06	euxassay_006887_07	euxassay_006887_08	euxassay_006887_09

euxassay_006887_10	euxassay_006887_11	euxassay_006887_12	euxassay_006887_13	euxassay_006887_14

euxassay_006887_15	euxassay_006887_16	euxassay_006887_17	euxassay_006887_18	euxassay_006887_19

euxassay_006887_20	euxassay_006887_21	euxassay_006887_22	euxassay_006887_23	euxassay_006887_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11476_wholemount_strong.wlz

11476_wholemount_moderate.wlz

11476_wholemount_weak.wlz

11476_wholemount_possible.wlz

11476_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11476_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7333
Entity Detected:	Slc35a1, solute carrier family 35 (CMP-sialic acid transporter), member 1 ( MGI:1345622)
Sequence:	sense strand is shown >T7333 CGCTTCCTCCTCGCGCGTGTGGTGCGGCGGGCTCTCTAGGCCGGCGCGTCTCTATGGCCGCAGGGGCGTC AGTTCCGCAGACTCTCTCGGCACCATGGCTCCGGCGAGAGAAAATGTCAGTTTATTCTTCAAGCTGTACT GCTTGGCGGTGATGACTCTGGTGGCTGCCGCTTACACCGTAGCTTTAAGATACACAAGGACAACAGCTGA AGAACTCTACTTCTCAACCACTGCCGTGTGTATCACAGAAGTGATAAAGTTACTGATAAGTGTTGGCCTG TTAGCTAAGGAAACTGGCAGTTTGGGTAGATTTAAAGCCTCATTAAGTGAAAATGTCTTGGGGAGCCCCA AGGAACTGGCGAAGTTGAGTGTGCCATCACTAGTGTATGCTGTGCAGAACAACATGGCCTTCCTGGCTCT CAGTAATCTGGATGCAGCAGTGTACCAGGTGACCTATCAACTGAAGATCCCCTGCACTGCTTTATGTACT GTTTTAATGTTAAATCGAACACTCAGCAAATTACAGTGGATTTCCGTCTTCATGCTGTGTGGTGGGGTCA CACTCGTACAGTGGAAACCAGCCCAAGCTTCAAAAGTCGTGGTAGCGCAGAATCCATTGTTAGGCTTTGG TGCTATAGCTATTGCTGTATTGTGCTCTGGATTTGCAGGAGTTTATTTTGAAAAAGTCTTAAAGAGTTCC GACACTTCCCTTTGGGTGAGAAACATTCAGATGTATCTGTCAGGGATCGTTGTGACGTTAGCTGGTACCT ACTTGTCAGATGGAGCTGAAATTCAAGAAAAAGGATTCTTCTATGGCTACACGTATTATGTCTGGTTTGT TATCTTCCTTGCTAGTGTGGGAGGCCTCTACACGTCAGTGGTGGTGAAGTATACAGACAACATCATGAAA GGCTTCTCTGCTGCCGCAGCCATTGTTCTTTCTACCATTGCTTCAGTCCTACTGTTTGGATTACAGATAA CACTTTCATTTGCACTGGGAGCTCTTCTTGTGTGTGTTTCCATATATCTCTATGGGTTACCCAGACAAGA TACTACATCCATTCAACAAGAAGCAACTTCAAAAGAGAGAATCATTGGTGTGTGATTTGAATCTCAAGAG ATTCCTATAAGGACTTAAACTGTTGATAATAAATTAGAGCCTTAAGTCAACCCCAGATGGTAGGTTAAAT AATGTCAACAAAATAATTGTATGACATAAGAATCAAGAAGAAAACTCTGAATGAAATGCTAAAACAGATT TAATTTGGGTGTGTTTGGTGTCAAGTTATATTATTTCAAAATGAAGGACTTTATATATATGAGAGAGAGA GAGAGAGAGAGAGAGAAAGAGAGAGAGAGATCTTGTACACAGAGCATGGAGGTGCCATGGTATTTTTGTG TGTGTGTGTGTGTGATGTACACAGAGCACGGAGGCAATGATGCAGGACCAGAGAGCATCAGGTGAAACTA TAATATTCAAGCAACGAGGTTTAAGACCGTGTCTGAGGGTTACAGTGCCAAAGCCATTTCTGTACACACT GTTCTCTTGTTCAGGTACCTGGAGAGGAAGGCTAGCCTTCTTCTCCAGTCCATGGATAGTACTTTGTCCC CATAGCAGTGAGGATCTAGCTTCTCTTCTCAGAGTGAGGAAGGAGTAAGACAGTTGAACACACCTCAGGG TGAATCTACTTCGTAGCTCAGACACTGACTTCTGGGTGAAGACTGAGAACTCTAGTGATGCATTTGTGCC ATTTACTGTCTGGTTCCTATTTCTTTGCTGTCAGCTGATATACTTTTCAGAAAATTTTATAAGCTGCTTT TATACTTTCTTTTTTATAAAGTATGGTTACCTGTTGGGCTCTCAATTTGTGACTTTCAGTGATTTTAAAA TATTTCTATAATGTTAATGGGGAAATCCAGCAATAAACTTATTTCTACCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4190458 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190458 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11474 same embryo
	EMAGE:11478 same embryo
	EMAGE:11477 same embryo
	EMAGE:11475 same embryo
	EurExpress:euxassay_006887 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source