EMAGE mouse gene expression spatial database

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EMAGE:11477

1110004E09Rik RIKEN cDNA 1110004E09 gene ( MGI:1915251)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006908. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006908_01	euxassay_006908_02	euxassay_006908_03	euxassay_006908_04

euxassay_006908_05	euxassay_006908_06	euxassay_006908_07	euxassay_006908_08	euxassay_006908_09

euxassay_006908_10	euxassay_006908_11	euxassay_006908_12	euxassay_006908_13	euxassay_006908_14

euxassay_006908_15	euxassay_006908_16	euxassay_006908_17	euxassay_006908_18	euxassay_006908_19

euxassay_006908_20	euxassay_006908_21	euxassay_006908_22	euxassay_006908_23	euxassay_006908_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11477_wholemount_strong.wlz

11477_wholemount_moderate.wlz

11477_wholemount_weak.wlz

11477_wholemount_possible.wlz

11477_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11477_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

strong

regional

strong expression: see section 14 15 16

cerebral cortex

strong

regional

strong expression: see section 13

choroid invagination

strong

regional

strong expression: see section 07 08 09 10 11 12 17 18 19 20 21

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7357
Entity Detected:	1110004E09Rik, RIKEN cDNA 1110004E09 gene ( MGI:1915251)
Sequence:	sense strand is shown >T7357 ATCTCAGGCTGGTGTGGGTCCGGCCGACCCGGCTCGGGCCTCGTGGTCCCTGCCCCGCAGCCTTGCCACC TCGGCGGCGCCTCTGGCGCAGCCATGGTTGTGCTGCACGTGAAGAGAGGCGACGAGAGCCAGTTCTTACT GCAGGCGCCCGGGAGCACGGAGCTGGAAGAGCTCACGGCGCAGGTGGCCCGCGTCTACAACGGGCGGCTG AAGGTGCACCGGCTCTGCTCAGAGATGGAGGAGCTGGCAGAACACGGTGTCTTTCTGCACCCTAATATGC AAGGGCTGACTGATGAGCAGATCGAAGAGCTGAAACTGAAGGACGAGTGGGGGGAGAAGTGTGTGCCCAG TGGAGGGTCTGTTTTTACGAAGGACGAGATCGGGCGGAGGAACGGGCAAGCTCCAAATGAGAAAATGAAG CAGGTTTTAAAGAAGACGGTAGAAGAAGCCAAGGCGATAGTGTCTAAGAAACAAGTAGAAGCTGGAGTCT TTGTTACTATGGAGATGGTGAAAGATGCCTTGGACCAGCTTCGAGGTGCGGTCATGATCGTTTATCCCAT GGGGCTGCCACCATACGATCCTATCCGGATGGAATTTGAAAATAAAGAAGATTTGTCAGGAACTCAGGCA GCCCTCGAAGTCATCCAGGAGTCGGAGGCACAGCTGTGGTGGGCCGCCAAGGAGCTGAGAAGAACAAAGA AGCTTTCTGACTATGTGGGGAAAAATGAGAAAACCAAAATCATCGTCAAGATCCAGCAGAGAGGGCAGGG TGCCCCAGCCCGGGAGCCTGTCATCAGCAGTGAAGAACACAAGCAGCTGATGCTGTTCTACCACAGAAGA CAGGAGGAGCTCAAGAAACTGGAAGAGAATGACGATGACTCCTGTCTGAACTCCCCGTGGGCTGACAACA CGGCTTTGAAAAGACATTTTCATGGCGTAAAAGACATAAAGTGGCGGCCCAGGTGAAGCCAACACTTGAC TGTCAGAACCTCAACCCACCAAATTCCTACCTTCCTGGTTTTCCTTTAGTTTGAATTTTTGAAATAAGTC ATTCACAGTTTTAGAACTTAATAAATACACATTCAAAAGAGAATGTTTTGGGCCAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191729 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191729 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11474 same embryo
	EMAGE:11478 same embryo
	EMAGE:11476 same embryo
	EMAGE:11475 same embryo
	EurExpress:euxassay_006908 same experiment
	MGI:4822588 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source