EMAGE mouse gene expression spatial database

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EMAGE:11478

Ngf nerve growth factor ( MGI:97321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006872. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006872_01	euxassay_006872_02	euxassay_006872_03	euxassay_006872_04

euxassay_006872_05	euxassay_006872_06	euxassay_006872_07	euxassay_006872_08	euxassay_006872_09

euxassay_006872_10	euxassay_006872_11	euxassay_006872_12	euxassay_006872_13	euxassay_006872_14

euxassay_006872_15	euxassay_006872_16	euxassay_006872_17	euxassay_006872_18	euxassay_006872_19

euxassay_006872_20	euxassay_006872_21	euxassay_006872_22	euxassay_006872_23	euxassay_006872_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11478_wholemount_strong.wlz

11478_wholemount_moderate.wlz

11478_wholemount_weak.wlz

11478_wholemount_possible.wlz

11478_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11478_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot skin

moderate

regional

moderate expression: see section 03 04 05 06 07 08 24

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7337
Entity Detected:	Ngf, nerve growth factor ( MGI:97321)
Sequence:	sense strand is shown >T7337 GCGCATCGAGTGACTTTGGAGCTGGCCTTATATTTGGATCTCCCGGGCAGCTTTTTGGAAACTCCTAGTG AACATGCTGTGCCTCAAGCCAGTGAAATTAGGCTCCCTGGAGGTGGGACACGGGCAGCATGGTGGAGTTT TGGCCTGTGGTCGTGCAGTCCAGGGGGCTGGATGGCATGCTGGACCCAAGCTCACCTCAGTGTCTGGGCC CAATAAAGGTTTTGCCAAGGACGCAGCTTTCTATACTGGCCGCAGTGAGGTGCATGGCGTAATGTCCATG TTGTTCTACACTCTGATCACTGCGTTTTTGATCGGCGTACAGGCAGAACCGTACACAGATAGCAATGTCC CAGAAGGAGACTCTGTCCCTGAAGCCCACTGGACTAAACTTCAGCATTCCCTTGACACAGCCCTCCGCAG AGCCCGCAGTGCCCCTACTGCACCAATAGCTGCCCGAGTGACAGGGCAGACCCGCAACATCACTGTAGAC CCCAGACTGTTTAAGAAACGGAGACTCCACTCACCCCGTGTGCTGTTCAGCACCCAGCCTCCACCCACCT CTTCAGACACTCTGGATCTAGACTTCCAGGCCCATGGTACAATCCCTTTCAACAGGACTCACCGGAGCAA GCGCTCATCCACCCACCCAGTCTTCCACATGGGGGAGTTCTCAGTGTGTGACAGTGTCAGTGTGTGGGTT GGAGATAAGACCACAGCCACAGACATCAAGGGCAAGGAGGTGACAGTGCTGGCCGAGGTGAACATTAACA ACAGTGTATTCAGACAGTACTTTTTTGAGACCAAGTGCCGAGCCTCCAATCCTGTTGAGAGTGGGTGCCG GGGCATCGACTCCAAACACTGGAACTCATACTGCACCACGACTCACACCTTCGTCAAGGCGTTGACAACA GATGAGAAGCAGGCTGCCTGGAGGTTCATCCGGATAGACACAGCCTGAGTGTGTGTGCTCAGCAGGAAGG CTACAAGAAGAGGCTGACTTGCCTGCAGCCCCCTTCCCCACCTGCCCCCTCCACACTCTCCTGGGCCCCT CCCTACCTCAGCCTGTAAATTATTTTAAATTATAAGGACTGCATGATAATTTATCGTTTATACAATTTTA AAGACATTATTTATTAAATTTTCAAAGCATCCTGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4190781 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190781 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11474 same embryo
	EMAGE:11476 same embryo
	EMAGE:11477 same embryo
	EMAGE:11475 same embryo
	EurExpress:euxassay_006872 same experiment
	MGI:4826698 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source