EMAGE mouse gene expression spatial database

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EMAGE:11479

Mtpn myotrophin ( MGI:99445)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006903. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006903_01	euxassay_006903_02	euxassay_006903_03	euxassay_006903_04

euxassay_006903_05	euxassay_006903_06	euxassay_006903_07	euxassay_006903_08	euxassay_006903_09

euxassay_006903_10	euxassay_006903_11	euxassay_006903_12	euxassay_006903_13	euxassay_006903_14

euxassay_006903_15	euxassay_006903_16	euxassay_006903_17	euxassay_006903_18	euxassay_006903_19

euxassay_006903_20	euxassay_006903_21	euxassay_006903_22	euxassay_006903_23	euxassay_006903_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11479_wholemount_strong.wlz

11479_wholemount_moderate.wlz

11479_wholemount_weak.wlz

11479_wholemount_possible.wlz

11479_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11479_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9288
Entity Detected:	Mtpn, myotrophin ( MGI:99445)
Sequence:	sense strand is shown >T9288 GCTTCTCCTCAGCTAGGCGGAACCTGCTCTGCTGGGCCCAACGGCTGCAGAAGAACTTTCTCCCGCCCGA ACCGTAGCCGCGACCCGCAGTCTCGCCCGCTCAGCGGCCTGAGGCGCGTCGCTTTCTCGTGTCCCCTCGT CGGGCCTAATCGCCGCTCCTGCCCGGCCCGCCGGCCCACCCCGCTGCAGTCATGTGCGACAAGGAGTTCA TGTGGGCCCTGAAAAACGGAGACTTGGATGAGGTGAAGGACTATGTGGCTAAGGGAGAAGATGTCAACCG GACACTAGAGGGTGGAAGGAAGCCTCTTCATTATGCTGCAGATTGTGGACAGCTTGAAATCCTGGAATTT CTGCTGCTGAAAGGAGCAGATATTAATGCTCCAGATAAACATCATATTACCCCTCTTCTGTCTGCTGTCT ATGAGGGTCATGTTTCCTGCGTGAAATTGCTTCTGTCAAAGGGTGCTGATAAGACTGTGAAAGGCCCAGA TGGACTGACCGCCCTTGAAGCCACTGACAACCAGGCAATCAAAGCTCTTCTCCAGTGATAGATGGATGGA CGGACTGATAACTTGGGAAGGATGACTCTCCTGTGGCCTCACACTGCTGCCTGTCTGTCTGTCACTTTCT ATCTGCCAGCTTCTTCAGCTAAATACTTTAAGAGGGTGAAGGGAGAGAGAAATTCATAACAAATCAGACT GCCAGGGGAGGGGAATAAAACATGTTTTGGAGGAGCAGTGGGGAATGTGATGAGACTTTTACTGGGATTC CTGATCAAACCAGCCTGTTTTTCATTTCCAGTAAAATATACAGCTTATTCCCAAGGGAGAGCAAAAATAC AGTCTATTGGAATCATTGGACCTTCTCAGCTCCCTAGCTAATTTATTAATTAGTTTATTGAAGGTCTGAC TATACAAGGCCAACTCTACTTATTTGGTAGCCCTAGCTGTGAGCAGTAGTGGCTGCTGTTATGTCAACAT AGGGTGCTGAGATAAGCAATTAGCTATAGCCTTCTGCCTTAAGAATGCACTCTCCTGGGGGCTCCTGGCT AGTTAAGAGCGGTTAGTGACCAAAATCTCTCTAGAATGACGTTAATCTTTACATAAGAGCTTAGATAAGG TAAGGAGCAGCGCACTCCTGAATTGCTGGATGACTAAACTTTGGATTTTCTCTCCCCTTTCACATCAATT TCATGTCTCTTTAGATAAAACTGACTAGTTTTATTTATAAAATGTTAAATTCTAGGAGCCTAAAGGAGGA ATCATTCCAGTAGGCTTATTTCTTTTTTTCTCCTTTAGACTCAGACATTTATATAACATTGAAACACTTT GAGACTCCTTCTGGTAGTAAAGGAGATTTAAAAATAAAATCATAGCCATAAGCGTGTTAGTATCTTATAA AGAGAGAACAGTTGTCTTAGTACCTATGGAGTTTCTTCATTTGCTATTTGAGTGAGCCAGCCTTCGTTGT GTTTGGAGAATGAAGAATATTCTTTGGAATGCCTCTCAAAAACCCACCATGGATGCTGATTTTATAGTGC ACCAAAGGTATCACTGGGGTGAGATGTACTGTATTTGGACAGATGGGACCTCATCCCTTCAAAAACATTC TTGTTACCATGTTCTCCTGAGCTGGTAATAGACTCTTGCTGGGCCAGTACCACCCCACCTGCATGGTGTT TTTATCTTAACAATCATTCTTTGCATACATGTGACAACTTCAAAATGCATTCTGTGTTCTTATAATACAG TGCCCTAAAACGGTATTCCTAGGACCTGCAGATAAGAGAAACTTATCTCTAACTTTTTCTTTCATGAACA ATAAAATGAATTAAAACATACAACCTTAAATTTGATGGCCTACACTTTAAGTATTAAGATGTCTTTTGCT TTTAATTTGCCATCTATACAGGATTACAAAAACTATTAGAATCTTTCCATAGCCAATCATGCAATAATTG AATGTACCTCAAATTTTTAAAGGGTAGGTGATGGGATAGGGACTTATAATCACATTAGGGATAATAATAT TAAAAACCTAACTGATTTTTTTTAAGGCATTGTAGCATTATATAGCAGGGTTAAACTATTACTAAGAACA ATCAGCCTGGCTAATAACTATTAATCAGTTGATAATTGGTTGGGGTGTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTGTGTGTGTGTTCTCCCATGAACTCTTTTTTATCCTGTGGCTTTTTCACTTTCAACTAGCCTG CCCCTGTAGTTTTTCTATGTCCAAGAAAACAATCACAAAACAGTAGTTTAAATACTTTGATATATTTGGC TAGCTCTGCTGTTTTAATGCAGCCTACTAGAGTTGGATATCCTACACTTAATCTTAACCAAAAGGCCAAG CAGCTATAGAGTTGGACATTCTGCTAGGAAGTGGAAGGCACATGATCAGTCTATGAGTCAATATAATGCA ACAGGGTGTTTTGAGACCTTTGTTTTCACCTCTTGGCATACTATTTTAAAGGCTTGAATTTTACCCTTTA CACAATTTTCAATTTTTCTTTCAAAGTCTTCTGTTATAGTTGGCTATTGCAGAGTGTATATTGTCTTATC ATTCCAGAACTTGGGTCTGCTTTCTACAGTCATTATGGTACCAAAACCATATGATTCTTTGTACAGTTTA TCCCAGTGTTCCTTGTAATGCAGTAGAGGCTATTTTGCCTTCCCCCTTTGTTGGCCATTTTGTAAACCCA TAGCTATGAAGTGATTGCTTGAGAAAATAGCATATAAACATAGACTAGGATAGACTGACCAGGATGGTTT ACAGTTTCTTTAAAAAAATAATAAACAAAAATAAACAAAAAAAAAACTAGGTTATTTATAATGTATTTCT GAACCACTTGGCAGAGAAATTCATGACACTTAATGTTAATATTTTTGAATAAATGAAGCTAAAATGTCTG CTTTTGGTACAACACGCATTAATAAAAGGTTAAGTTTTGTTTTCCACTGAAGTACTATTTAACATCTCAG AAAAAAACCCTGCATGTTCTATAGTTTTATATTAAAATCCATCATTTCATATGCACTGTATCAAAAACAG GTTACTTGCCTGAACATGGTTAGTGTACTAACAGGTCTGCCCACCCCTACCCTCACCCCCAGCTTCATGC CAGCATATGTAGATTTGAGTTCTAACACAGCACTTGCATATCCCATGTGCACAAGCTCACCTTTCCAGAA CCAGCTGGGCGCAGAAGAAAGGCAGTAACTGCAGCATGTGAAAGCTAAAACAAGTAGCCTTCTAGTCATT TCTTTCTTTCCCCAGCCAGATAAAAGGAGCTTCAATATATGTGTACTTGATTTTTATTCACTTCAGCTGA GCTGCTGTTTCCTTCATGTAATATTGTATACTGGGTTGTGTATAGAAGAAGCTGGTAAGAGTGCCCTCCT TCATAAATAAGCAATTGCAGTGTTTTGCATGCAAATTTAAAAAAATTTAAATTGTCCTGATTCTATTTTG TAAATGGAGAAACAATCTTTCTAAGCAGTATGGAGGAAGACTAGTGCTTTGTGCACTTTTGGTATATTTG AGTTCATGTTTCCACAATGTCATTCCCTTGACACAGTTGGGTTTCTCATAAGTATCCTAGTTCATGTACA TCCGAATGCTAACTAATACTGTGTTTAAGTTTCGTGTTGCAAGAACAAATGGAATAAACTTGAATTGTGC TATAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839739 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839739 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11480 same embryo
	EMAGE:11482 same embryo
	EMAGE:11481 same embryo
	EurExpress:euxassay_006903 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source