EMAGE mouse gene expression spatial database

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EMAGE:11482

Enc1 ectodermal-neural cortex 1 ( MGI:109610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006919_01	euxassay_006919_02	euxassay_006919_03	euxassay_006919_04	euxassay_006919_05

euxassay_006919_06	euxassay_006919_07	euxassay_006919_08	euxassay_006919_09	euxassay_006919_10

euxassay_006919_11	euxassay_006919_12	euxassay_006919_13	euxassay_006919_14	euxassay_006919_15

euxassay_006919_16	euxassay_006919_17	euxassay_006919_18	euxassay_006919_19	euxassay_006919_20

euxassay_006919_21	euxassay_006919_22	euxassay_006919_23	euxassay_006919_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11482_wholemount_strong.wlz

11482_wholemount_moderate.wlz

11482_wholemount_weak.wlz

11482_wholemount_possible.wlz

11482_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11482_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9126
Entity Detected:	Enc1, ectodermal-neural cortex 1 ( MGI:109610)
Sequence:	sense strand is shown >T9126 CGCCAGGCGGCCGCCAGCGCTCACGCCGCAACCCCGTTCCCTCCCCCTGCGTGCAGACCGCCTTCCTCCG CGGCGCGGCCGCCGCAGGCTCACTCTCCCCTGGCTAGGCGCGCGCCGGCAGACGCTTGGCCCGGCCGGGG CGCCAGGTCCGTAGCAGCAGATCCGGAGACCGAGGTCAGCTGCCTGCCGAAACCAGCCCGGGAGCCACTA CCCCGGACGCTATCGCCTCCCTTCCCAGCGCTCCTTTCTCCCCGGCGATCGCCACCGCTTCCTGGAACAG AGACGCCCTTCGTCGGCTCCCTGCGGGTGACCGAGGCAAGAACCGCAGCCGGTTGTGTGCAGAGGCCAGC CGCCACTACTGAGTTGAAACAAAATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGTGG CTCCATCAACATCTACCTGTTTCATAAGTCCTCCTACGCGGACAGCGTTCTCACTCACCTGAACCTTCTG CGTCAGCAGCGGCTCTTCACAGATGTCCTTCTCCATGCGGGAAACAGGACCTTCCCTTGCCACCGGGCAG TGCTGGCTGCGTGCAGCCGCTACTTCGAAGCCATGTTCAGTGGTGGCCTGAAAGAGAGCCAGGACAGTGA GGTGAACTTCGACAATTCCATCCACCCAGAAGTCTTAGAGCTGCTTCTAGACTACGCATACTCCTCCCGG GTCATTATCAATGAAGAAAATGCTGAGTCGCTCCTGGAGGCTGGTGACATGCTGGAGTTCCAGGACATCA GAGATGCGTGTGCAGAATTTCTAGAGAAGAACCTGCATCCCACCAACTGCCTGGGTATGCTGCTGTTGTC TGATGCCCACCAGTGCACCAAGCTGTACGAACTCTCCTGGAGAATGTGTCTCAGCAACTTCCAAACCATT CGGAAGAACGAAGATTTCCTCCAGTTGCCCCAGGACATGGTTGTGCAGCTGCTGTCCAGTGAAGAACTGG AGACGGAAGACGAAAGGCTGGTGTATGAGTCTGCGATGAACTGGATCAGCTATGACCTGAAGAAGCGCTA CTGTTACCTCCCGGAACTGTTGCAGACAGTGAGGCTGGCCCTCCTTCCTGCCATCTATCTCATGGAGAAC GTGGCGATGGAAGAACTCATCACCAAGCAGAGAAAGAGTAAGGAGATCGTGGAAGAGGCCATCAGGTGCA AACTAAAAATCTTACAGAATGACGGCGTGGTCACCAGTCTCTGTGCTCGTCCTCGGAAAACTGGCCATGC CCTGTTCCTCCTGGGAGGGCAGACTTTCATGTGTGACAAACTGTACTTGGTAGACCAGAAGGCTAAAGAA ATCATTCCCAAGGCTGACATTCCCAGCCCGAGGAAAGAGTTCAGCGCATGTGCAATTGGCTGCAAAGTAT ATATTACTGGGGGGCGGGGATCAGAGAACGGAGTCTCAAAAGATGTCTGGGTTTACGATACCCTGCATGA GGAGTGGTCCAAGGCTGCCCCCATGCTGGTGGCCAGGTTTGGCCATGGATCTGCTGAACTGAAGCACTGC CTCTATGTAGTCGGTGGGCACACAGCTGCAACTGGCTGCCTCCCAGCCTCCCCCTCGGTCTCCCTAAAGC AAGTAGAACAGTATGACCCCACAACCAACAAATGGACCATGGTAGCCCCACTCCGCGAAGGTGTCAGCAA TGCTGCTGTAGTGAGTGCCAAACTTAAGCTGTTTGCTTTCGGGGGTACCAGTGTGAGCCACGACAAGCTG CCCAAGGTTCAGTGTTACGATCAATGCGAGAACAGATGGTCAGTGCCGGCCACCTGTCCCCAGCCCTGGC GTTACACAGCCGCAGCTGTGCTGGGAAACCAGATTTTTATCATGGGTGGAGATACAGAGTTCTCTGCCTG CTCCGCTTACAAATTCAATAGTGAGACTTACCAGTGGACCAAGGTGGGAGATGTGACAGCCAAGCGCATG AGCTGCCACGCCGTGGCGTCCGGGAACAAGCTTTACGTAGTTGGAGGGTACTTCGGCATTCAGCGCTGCA AGACTTTGGACTGTTACGACCCAACTTTAGATGTGTGGAACAGCATAACCACTGTTCCCTACTCTCTGAT CCCTACTGCATTCGTCAGCACCTGGAAACACCTGCCTTCCTAATGTAGAGCATCCTAAAGAAAGCACGCA TGAGCTCATTCTGATGCACGGCGAGATGAGATGTCATTTCTGCTTTGGAGAAGGCAAGTTTAATGAAGAG AAAGAAAAACAGGAGCAGTTGCTACTGAGACTCCTCGAATACCATCGGCTGCACCTTGCAAACACTCTCA AGTGGACATGAAGGAAGGGGTGGGGGGAGGGGCGGGTTTTTTTTTTTCAATATAAGTAGCACATGGTTTA AATATGAATGACTGAACTTGTGATCTAGTCCTTGTCTGGTAATTGTGGATTAATGTCAGCGTTAATCAGC CCCTCAAAGGGAGAGAAAAGCTGGGCTTTTCCCTTGCTGTACCTCATTCAGCTTTTGATTTCCATGGCCC CACCATTTATGTGCAAGATTTGCAATGGTTGTCAGCTTCCTCTGAAGACCGAGCTTGACGCCTCCATGCC AGCTGCCGTTGGAGACGCAAAGCCAAGCAAGGGTCAGGAGGGAAGCTGGCCCGGCTGACTGGAGAATGGG AACCCCAGGACTCTCCACTCATCTCGAAGGGTTGTGGTCCCCCCAGGAAAGTCTCGAGACAATGGGGACA TTGTTGGTAGCTGTTCGGTAGATGCTCTTTGCTATTTATAAGTGACTTTAAACCTTCTCTTGGCTGTTAA GAAATGTGTTCTAGATTTAGCTATTTATTGTTTGCGGCCTGCATGCTGAAACAGTGCTTACGTTGTCTCC ATGTGTACGGGGCCTGTGTGGATGGTCGTATGTTTTGCACATTTTGTAGTTGTTGGTGTGCTTCGCCGCA CACAAAAAAAGAAGACTTTGGGGCTGTAATTTGGAGGATAAGTAGAGGGTGGGTGGGGGGAGGGGTACAT TTTTGAAGTGTCGGGACAGAGACAGATTCCAAGACTGGAAACTTCAATTACATCCAGAGGCAGAGAAACC TTTCCTCGACCACTCTTTATTGAGGTACCCTGGTCACACTGATGGCAGGACACACAAGAGCAGGGTATTG GTCTGCCCTTCTTCCTCCTGTCCGTCTGATTTACCTAACTTTGGTCTCGGCTGCTGACTCTTGGAGAGGA CCAAATTACTTTGTAGTATTTCCCCCGTTTGTGTAATGTCCTGAAACCTTGGAGAGGTTCCAGAATACTT CTGTATATAGGGCACAGGTGAAGACATTGTCCAAAGCTTATTTATTTATCTATTTATTTACCCTGGGCGA GTAACCACACCAGTGGGGGGGAAAAAACTAAAATGTGTGGAGTGTAAACAAACTCACTGACCACGTGGCT CTTGGACCTTGATTTAGAAAGGCTCATACTCCCAGGAAAACACCCATTTTGATACAATGTAAACGTTCGT GTTCACCCTTGGATACATGTTGAAAGAGCTCTTTGGTACACGGAAGTGACATCTTTGGGGTCAGTGTTAC GAGGTGGCCTGTGGAGGTCTTCCGGTCCTGTTGGGATCATTTGTGAATACATTCTTTGCTCCTCTTAAGT GCTTGTTTCCTAACCGTGCAGTGGTAGGTATTAGTACACTGGGCACTTCCCCAGGGATCTGGGGAAGACC AGAGCTTGCAGCTCTGCCTGCTTTGATCCATATTTCTCACAGTGCTGTATTAAAAAATAGAACCTAAGGC AGATAGCCACCTTTACATTGTGAGTGTGTTTACCTTGTCTAATGACGGATAATTCCTTAACATTTCTCTC CACCTTAGTACTTTAGGCTAATTATACACGTCTGTCTGTGCCATGAGTAAGTGGACTGTAGTTGGACCAA AAAAACAAATGAGCCGTTGGACCATTTGAGCAGTCAGTTTCTGGTCCTTAGATGTATTCTAAGCAGTGTA AGTGTCTGATTGTACCCTGGTGGCATGATCAGTTGTCTTGTAGCTGTCCCCCGCTCCATAGTTTACAATG CAAATCTGTCTCAAGATCTTCACTTCGCTGCTGCTGAGAGCAGGAGAATTTTCTGCAGCTGTTTCAAAGT TGTGGCCTGGCCTTGAATCCTCTATTAATTACTGTGTGAGCCAGAGGGAACTGCCCTGCAAGGGTGGGCC CCCAGCCGGCAGGGGAACTTTCTAGACTCCCGCTCATTCAATTGATCTAGGCATTTGGGCTTGCTACTTG ACCATTCTCGCCCTGTGAAATGTCCCACACTTTGAAGCAAATACAATTCACAGCACAGTACACACAAAAA CCCTGGCATAAGACGGGGAGGTTCTTCTTATTTTGTGAGCCGGTTGCCCTGGAAACGGATAACAAAGGGC AGCCTTCCACTTCTGGTATAATGGGGGAGCCTCTTTTCTCAGGCTTGACACCTGTCTGAATAAGAGTGAT TAGAGCCGCATAATATCCCTCTCTTGGCTATTGAATAAGTGGTTCACATACCCAACCCTGTAGAAGTTAG AAGATGGTCGGTCATGTCTGTATGTTCTTGTTTGCATCTACTACATTTTTAAGGTTTTGTAAAACTGTTA TTTTTTTTTCACGATGTGAAACTGAAGGTCAATAAATTATTAGAGATTTTCAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:6824970 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6824970 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11480 same embryo
	EMAGE:11479 same embryo
	EMAGE:11481 same embryo
	EurExpress:euxassay_006919 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source